Chitosan artificial nerve graft containing neurotrophic factor and preparation method thereof
A technology of neurotrophic factors and artificial nerves, which is applied in the field of medical devices, can solve the problems of limited tensile strength of nerve conduits, large product apertures, and cumbersome steps, and achieve the effects of long-term storage and transportation, guaranteed activity, and a good growth environment
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Embodiment 1
[0026] Chitosan was dissolved with lactic acid with a concentration of 0.5% to obtain a solution with a concentration of 3%. Then the solution was injected into the mold, and after pre-forming by freezing at -60°C, the material was withdrawn from the mold and put into 5% sodium hydroxide solution to neutralize the acid in the material. After cleaning, it was placed in a 15 μg / ml nerve growth factor (NGF) solution and fixed for 30 minutes using the abundant pores on the surface of the material. The chitosan artificial nerve graft containing neurotrophic factor is then obtained by freeze-drying, with a porosity of 89% and an average pore diameter of 87-98 μm.
Embodiment 2
[0028]Chitosan was dissolved with 8% acetic acid to obtain a 5% chitosan solution. Then the solution was injected into the mold, and after pre-forming by freezing at -80°C, the material was withdrawn from the mold and put into a 10% sodium carbonate solution. After the acid in the neutralized material was fixed and molded, the excess alkali and the resulting salt were removed with distilled water. After cleaning, the chitosan artificial nerve graft is obtained by freeze-drying. Before use, the chitosan artificial nerve grafts were placed in a 20 μg / ml solution of basic fibroblast growth factor (bFGF) and fixed for 1 hour using the abundant pores on the surface of the material. The chitosan artificial nerve graft containing neurotrophic factor is then obtained by freeze-drying, with a porosity of 77% and an average pore diameter of 55-67 μm.
Embodiment 3
[0030] Dissolve chitosan with phosphoric acid with a concentration of 2% to obtain a solution with a concentration of 6% chitosan, and then inject the solution into the mold, after pre-forming by freezing at -90 °C, the material is withdrawn from the mold and placed in 5% sodium hydroxide In the solution, after the acid in the neutralized material is fixed and formed, the excess alkali and the generated salt are washed with distilled water, and then freeze-dried to obtain the chitosan artificial nerve graft. Before use, the chitosan artificial nerve grafts were placed in a 20 μg / ml brain-derived neurotrophic factor (BDNF) solution and fixed for 2 hours using the abundant pores on the surface of the material. Then, the chitosan artificial nerve graft containing neurotrophic factor is obtained by freeze-drying, with a porosity of 53% and an average pore diameter of 11-23 μm.
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