79 results about "Streptomyces coelicolor" patented technology

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

The use of enzymes that catalyze the production of starter and extender units for polyketides in E. coli and Streptomyces is described; these enzymes include malonyl CoA decarboxylase (MatA), malonyl CoA synthetase (MatB), and a malonate transporter (MatC) as well as proprionyl CoA carboxylase (pcc). The matBC gene from Streptomyces coelicolor, the matABC genes from Rhizobium trifoli, and the pccB and accA2 from Streptomyces coelicolor are useful in specific embodiments of the claimed invention. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. coli in the presence of a phosphopantetheinyl transferase, such as sfp from Bacillus subtilis. This achievement permits a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.

Constructs for expressing herbicide tolerance genes, related plants, and related trait combinations, said constructs comprise a gene referred to herein as DSM-2 identified in Streptomyces coelicolor (A3). The DSM-2 protein is distantly related to PAT and BAR DSM-2 can be used as a transgenic trait to impart tolerance in plant cells and plants to the herbicidal molecules glufosinate, phosphinothricin, bialaphos, and / or the like. The subject invention also relates to combination of the subject herbicide tolerant crop (HTC) traits along with other HTC traits and / or insect resistance (IR) traits.

The invention provides construction and application of a multiple gene co-expression system containing angolosamine glycosylsynthetase and glycosyltransferase. The invention is characterized by taking a streptomyces genome bank plasmid as a template; amplifying six angolosamine synthetase genes and a glycosyltransferase gene through PCR and connecting the genes in sequence and placing the genes in the lower reaches of a streptomyces promoter PactIII-actI to form a transcription unit; transferring the transcription unit to a streptomyces plasmid carrier pSET152, thus constructing a streptomyces expression plasmid pAYT55 co-expressed by multiple genes; leading the pAYT55 into a host cell streptomyces coelicolor CH999, mixedly culturing obtained engineering bacteria and streptomyces B135 of accumulated polyketide kalafungin, thus realizing bioconversion of kalafungin into a novel antibiotic with angolosamine; or directly leading pAYT55 into the streptomyces B135 and carrying out single culture to realize glycosylation of kalafungin. Therefore, by adopting the system, rare angolosamine can be synthesized in the cell and angolosamine modification can be carried out on polyketide by adopting the low substrate recognition specificity of antibiotic glycosyltransferases.

The subject invention relates in part to constructs for expressing herbicide tolerance genes, related plants, and related trait combinations. Such constructs and plants comprise a gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor (A3). The DSM-2 protein is distantly related to PAT and BAR. DSM-2 can be used as a transgenic trait to impart tolerance in plant cells and plants to the herbicidal molecules glufosinate, phosphinothricin, bialaphos, and / or the like. The subject invention also relates to combination of the subject herbicide tolerant crop (HTC) traits along with other traits, including other HTC traits and / or insect resistance (IR).

ADP-ribosylating toxins from Neisseria meningitidis, Streptomyces coelicolor, Mycoplasma pneumoniae, Salmonella typhimurium, Salmonella paratyphi, and Streptococcus pyogenes are disclosed, together with mutant toxins and uses therefor. There is only a low level of sequence identity between these toxins and toxins such as cholera toxin and E. coli heat labile toxin.

The invention relates to a nsdBmgh gene of streptomyces roseoflavus (Men-myco-93-63) as well as a preparation method and application thereof. The preparation method of the nsdBmgh gene of streptomyces roseoflavus comprises the following steps: automatically designing a primer Ns8F and Ns8R through the sequence of a negative control gene nsdB in the metabolic pathway of sky blue streptomycete A3(2) antibiotic, amplifying genomic DNA of the streptomyces roseoflavus (Men-myco-93-63) to obtain fragments of 1121bp, and then extending the two ends of a target sequence by utilizing a nonspecific primer anchoring PCR(NPA-PCR) method so as to obtain the nsdBmgh gene of the streptomyces roseoflavus (Men-myco-93-63). The functions of the nsdBmgh gene are verified through the gene disruption, and the bacteriostatic activity of the gene disruption mutant strain is obviously enhanced as compared with that of metabolin of original strains. The preparation and the application of the nsdBmgh gene provide an important basis for further researching and verifying the synthetic and metabolic pathway and the adjusting and controlling mechanism of the streptomyces roseoflavus, and lay the foundation for the application of high-yield genetic engineering strains of antibiotics for preventing plant diseases.