Streptomyces coelicolor trehalose synthase enzyme gene and application thereof

Abstract

The invention relates to a streptomyces coelicolor trehalose synthase enzyme gene. The gene is cloned from streptomyces coelicolor (Streptomyces coelicolor, AS 4.1061). A trehalose synthase enzyme expressed by the gene can be used for converting maltose into trehalose under the normal biochemical reaction conditions and has the characteristics of high reaction speed, high conversion rate of a substrate and few byproducts. The content of trehalose in a product generated in the process of converting the maltose at a temperature of 25 DEG C can achieve over 75 percent and only little glucose is generated. The streptomyces coelicolor trehalose synthase enzyme gene is beneficial for improving the conversion rate of the substrate and reducing the production cost. The gene can be applied to production of the trehalose. The trehalose can be applied to the fields of food industry, beauty cosmetics, medical biological products and the like.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and modern enzyme engineering, and specifically relates to a trehalose synthase gene of Streptomyces coelicolor and its application. Background technique [0002] Trehalose (Trehalose) is a non-reducing disaccharide formed by connecting two molecules of glucose with α, α-1, 1-glycosidic bonds. It was first extracted from ergot bacteria in rye by Wiggers in 1832, and then Recent studies have found that trehalose widely exists in microorganisms, shrimp, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. Exogenous trehalose also has a good non-specific protective effect on organisms, biological macromolecules, and biofilms. It can protect cells from damage caused by environmental changes, such as dehydration and hypertonic changes. Effective protection of biofilm, protein and DNA, etc., so it is known as "sugar of life". The content of trehalose in nature is ve...

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Problems solved by technology

[0004] At present, many literatures and patents have reported the cloning and isolation of genes related to trehalose synthesis, and the use of related enzymes of these trehalose synthase genes to prepare trehalose, but the synthesis pathways of these enzymes related to trehalose synthesis are different, or bacteria The different sources of species lead to very different DNA sequences and amino acid sequences, as well as different enzymatic properties and enzyme activities, and most of them have not yet been applied on an industrial scale.

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