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Method for improving production of Streptomyces antibiotics by using nanomaterial as additive

A technology of nanomaterials and additives, which is applied in the field of increasing the production of antibiotics, can solve the problems of cumbersome production methods of antibiotics, and achieve the effect of simple and easy method and strong operability

Active Publication Date: 2019-06-14
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the cumbersome problem of using existing methods such as molecular biology and mutagenesis to increase the production of antibiotics, and to provide a method for improving the production of antibiotics in Streptomyces by using nanomaterials as additives

Method used

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  • Method for improving production of Streptomyces antibiotics by using nanomaterial as additive
  • Method for improving production of Streptomyces antibiotics by using nanomaterial as additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method of using nano-alumina as an additive to improve the antibiotic production ability of Streptomyces coelicolor M145 includes the following steps:

[0021] 1) Streptomyces coelicolor M145 (Bentley SD et al., 2002, Complete genome sequence of themodel actinomycete Streptomyces coelicolor A3(2). Nature, 417:141-147) was spread on the solid medium of soybean meal (MS), and After culturing for 7 days at 30°C, the spores were collected and filtered using a cotton swab, suspended in a 20% (V / V) glycerol solution, and stored at -80°C.

[0022] The composition and preparation method of the MS medium are as follows: Weigh 20g of soybean meal powder, add 800mL of tap water and boil for half an hour, use six layers of gauze to filter out the filter residue, add 20g of mannitol, 20g of agar powder, and make up to 1L of tap water. Adjust the pH to 7.2-7.4 and sterilize at 121°C for 20 minutes.

[0023] 2) Nano alumina (CAS No.: 1344-28-1) was purchased from Shanghai Maclean Bioche...

Embodiment 2

[0038] The method of using nano-copper as an additive to improve the ability of Streptomyces coelicolor M145 to produce antibiotics includes the following steps:

[0039] The nano material is changed to nano copper, and the rest is the same as in Example 1. Nano copper (CAS number: 7440-50-8) was purchased from Shanghai Macleans Biochemical Technology Co., Ltd.

[0040] In the embodiment of the present invention, the concentrations of nano-copper were set to 0, 5, 10, 20, 50 and 100 mg / L, and cultured for one week. The measured concentrations of actinopurin and undecyl prodigulin are shown in Table 3. As shown in Table 4:

[0041] Table 3: The amount of actinomycin produced by Streptomyces coelicolor M145 after treatment with 0-100mg / L of nano-copper (mg / L)

[0042]

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5mg / L

10mg / L

20mg / L

50mg / L

100mg / L

72h

0.14±0.02

0.16±0.02

0.24±0.03

0.11±0.02

0.08±0.02

0.06±0.01

96h

0.34±0.08

0.57±0.09

0.87±0.09

0.25±0.03

0.22±0.03

0.11±0.02

120h

0.92±0.11

1.57±0.08

1.7...

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Abstract

The invention relates to a method for firstly using a nanomaterial as an additive to increase the production of Streptomyces antibiotics. Compared with a traditional method of increasing the production of antibiotics, the method is simple and easy to operate, and has high operability. The method adds Streptomyces spores to a well-prepared YBP liquid medium containing different concentrations of nanoparticles, and ferments the materials for about one week, and during the period, the condition of the strain is regularly determined to produce two colored pigments, actinorhodin and undecylamine. For the first time, the method is applied to the antibiotic fermentation of Streptomyces as the additive, and the method is used to culture Streptomyces coelicolor, and the method found that the addition of 1000 mg / L of nano-alumina increases the yield of actinorhodin by 4.6 times and the yield of undecylprodigiosin by 3.7 times. The method found that the addition of 10 mg / L of nano copper increases the yield of actinorhodin by 2.1 times and the yield of undecylprodigiosin by 1.8 times.

Description

Technical field [0001] The invention belongs to the technical field of improving the yield of antibiotics, and specifically relates to the addition of nanomaterials that can improve the ability of Streptomyces coelicolor M145 to produce two antibiotics. Background technique [0002] Among the naturally produced antibiotics, antibiotics produced by actinomycetes account for about 2 / 3 of them. Among them, as a typical representative of actinomycetes, antibiotics produced by streptomyces account for the vast majority, accounting for about 80% ( LpezGarce et al., 2014, Kieser et al., 2000), and more than 100 kinds have been used in clinical practice. At present, these antibiotics have been widely used in anti-bacterial, anti-fungal, anti-tumor, and prevention and treatment of pests and diseases. For example, Jinggangmycin, Zhongshengmycin, Kasugamycin, etc. used for the prevention and control of plant diseases and insect pests, Abamectin used for killing parasites and mites, FK506 a...

Claims

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Application Information

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IPC IPC(8): C12P17/16C12R1/465
Inventor 唐景春刘小妹
Owner NANKAI UNIV
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