Microbe microbial inoculum, preparation method thereof and method for producing biological humic acid
A technology for microbial inoculants and bacilli, applied in the field of microbial inoculants and their preparation and production of biological humic acid, can solve the problems of air and natural environment pollution, secondary environmental pollution, disturbance, etc. The effect of high activity and small molecular weight
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[0019] The present invention also provides a preparation method of microbial inoculum, wherein, the method comprises inoculating Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Streptomyces and Aspergillus fumigatus in culture medium, so that each obtained The total number of live bacteria in gram of microbial agent is 0.5-1.5×10 9 One, wherein, the number of viable bacteria of the Bacillus subtilis is 10-30% of the total number of viable bacteria, the number of viable bacteria of the Bacillus amyloliquefaciens is 10-30% of the total number of viable bacteria, and the number of viable bacteria of the Bacillus licheniformis The total number of viable bacteria is 10-30% of the total number of viable bacteria, the number of viable bacteria of the Streptomyces is 5-15% of the total number of viable bacteria, and the number of viable bacteria of the described Aspergillus fumigatus is 5% of the total number of viable bacteria -15%.
[0020] In the present inv...
Embodiment 1
[0035] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.
[0036] (1) Inoculate 3 parts by weight of Bacillus licheniformis strain (ACCC11080, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient gravy medium, ferment and cultivate at 37 ° C, sample during fermentation and pass Microscopic direct counting method was used to observe until the number of live bacteria of Bacillus licheniformis was 0.5×10 9 culture medium per gram, wherein, in the gravy culture medium, the content of peptone is 0.5% by weight, the content of NaCl is 0.5% by weight, the content of beef extract is 0.3% by weight, the balance is sterile water, and the pH is 7.0.
[0037] (2) Inoculate 4 parts by weight of Bacillus subtilis strain (ACCC11089, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient gravy medium, ferment and...
Embodiment 2
[0043] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.
[0044] (1) Inoculate 100 parts by weight of the nutrient gravy medium with 5 parts by weight of the bacterial strain of Bacillus licheniformis (ACCC11080, purchased from the China Agricultural Microorganism Culture Collection Center), ferment and cultivate at 37 ° C, sample during the fermentation and pass Observation by microscope direct counting method until the number of live bacteria of Bacillus licheniformis was 1.5×10 9 culture medium per gram, wherein, in the gravy culture medium, the content of peptone is 0.5% by weight, the content of NaCl is 0.5% by weight, the content of beef extract is 0.3% by weight, the balance is sterile water, and the pH is 7.0.
[0045] (2) Inoculate 3 parts by weight of Bacillus subtilis strain (ACCC11089, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient ...
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