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Microbe microbial inoculum, preparation method thereof and method for producing biological humic acid

A technology for microbial inoculants and bacilli, applied in the field of microbial inoculants and their preparation and production of biological humic acid, can solve the problems of air and natural environment pollution, secondary environmental pollution, disturbance, etc. The effect of high activity and small molecular weight

Active Publication Date: 2010-06-02
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the direct combustion of sugarcane sugar residue is the most simple and fast method to deal with sugarcane residue, the direct combustion of sugarcane residue not only wastes sugarcane residue resources, but also produces a large amount of CO 2 and other greenhouse gases, causing serious pollution to the air and natural environment
A large amount of waste water will be generated during the papermaking process of sugar cane sugar residue, which will cause secondary pollution to the environment
[0006] Since the content of lignin in sugarcane sugar residue is as high as 20%, direct use as feed can cause damage and disorder to the digestive system of animals. Currently, the domestic use of sugarcane residue to feed cattle, sheep and other animals is limited, and the dosage No more than 20% of the diet, thus limiting the development and utilization of sugar cane residues as feed

Method used

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  • Microbe microbial inoculum, preparation method thereof and method for producing biological humic acid
  • Microbe microbial inoculum, preparation method thereof and method for producing biological humic acid
  • Microbe microbial inoculum, preparation method thereof and method for producing biological humic acid

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preparation example Construction

[0019] The present invention also provides a preparation method of microbial inoculum, wherein, the method comprises inoculating Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Streptomyces and Aspergillus fumigatus in culture medium, so that each obtained The total number of live bacteria in gram of microbial agent is 0.5-1.5×10 9 One, wherein, the number of viable bacteria of the Bacillus subtilis is 10-30% of the total number of viable bacteria, the number of viable bacteria of the Bacillus amyloliquefaciens is 10-30% of the total number of viable bacteria, and the number of viable bacteria of the Bacillus licheniformis The total number of viable bacteria is 10-30% of the total number of viable bacteria, the number of viable bacteria of the Streptomyces is 5-15% of the total number of viable bacteria, and the number of viable bacteria of the described Aspergillus fumigatus is 5% of the total number of viable bacteria -15%.

[0020] In the present inv...

Embodiment 1

[0035] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.

[0036] (1) Inoculate 3 parts by weight of Bacillus licheniformis strain (ACCC11080, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient gravy medium, ferment and cultivate at 37 ° C, sample during fermentation and pass Microscopic direct counting method was used to observe until the number of live bacteria of Bacillus licheniformis was 0.5×10 9 culture medium per gram, wherein, in the gravy culture medium, the content of peptone is 0.5% by weight, the content of NaCl is 0.5% by weight, the content of beef extract is 0.3% by weight, the balance is sterile water, and the pH is 7.0.

[0037] (2) Inoculate 4 parts by weight of Bacillus subtilis strain (ACCC11089, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient gravy medium, ferment and...

Embodiment 2

[0043] This example is used to illustrate the microbial inoculum provided by the present invention and its preparation method.

[0044] (1) Inoculate 100 parts by weight of the nutrient gravy medium with 5 parts by weight of the bacterial strain of Bacillus licheniformis (ACCC11080, purchased from the China Agricultural Microorganism Culture Collection Center), ferment and cultivate at 37 ° C, sample during the fermentation and pass Observation by microscope direct counting method until the number of live bacteria of Bacillus licheniformis was 1.5×10 9 culture medium per gram, wherein, in the gravy culture medium, the content of peptone is 0.5% by weight, the content of NaCl is 0.5% by weight, the content of beef extract is 0.3% by weight, the balance is sterile water, and the pH is 7.0.

[0045] (2) Inoculate 3 parts by weight of Bacillus subtilis strain (ACCC11089, purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of nutrient ...

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Abstract

The invention provides a microbe microbial inoculum and a preparation method thereof as well as a method for producing biological humic acid with sugarcane sugaring residue by the microbe microbial inoculum. The microbe microbial inoculum prepared by the invention comprises a culture medium and thallus, wherein the thallus comprises Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Streptomyces coelicolor and Aspergillus fumigatus, and each gram of compounded microbe microbial inoculum contains 0.5-1.5*109 total viable bacteria. The microbe microbial inoculum of the invention can quickly ferment sugarcane sugaring residues so as to obtain biological humic acid. Compared with mineral humic acid, the biological humic acid has the advantages of small molecular weight, large amount of active group, favourable water solubility, high bioactivity and the like and is easy to adsorb by the tissues of plants and animals, so that the biological humic acid can serve as the repairing preparation and fertilizer of water body and soil. Thus, the sugarcane sugaring residue can be effectively processed and utilized so as to really change waste material into things of value.

Description

technical field [0001] The invention relates to a microbial bacterial agent, a preparation method thereof, and a method for producing biological humic acid through sugar cane residues by using the microbial bacterial agent. Background technique [0002] Sugarcane is an important sugar crop and the main source of sugar in my country. [0003] In the process of sugar production from sugar cane, most of the sugar is extracted, while most of the remaining non-sugar organic matter exists in the by-products, forming residues. The residue contains the fibrous material of the sugarcane stem after crushing and extracting sugarcane juice from sugarcane, which is about 25% of the weight of sugarcane. my country produces nearly 10 million tons of sugarcane sugar residue every year. [0004] At present, sugarcane sugar residue is mainly used as fuel for power generation and steam supply in sugar factory boilers, and a small part is used for papermaking and animal feed production. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/14C12P1/00C12R1/125C12R1/07C12R1/10C12R1/465C12R1/68
Inventor 朱昌雄叶婧刘丽辉郭萍俞晓芸李瑞波田云龙
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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