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Mixed bacterial agent capable of degrading antibiotics in soil and preparation method thereof

A technology of mixing bacterial agents and antibiotics, applied in the field of microorganisms, can solve problems such as low degradation rate, limited degradation range, and agricultural antibiotic pollution.

Inactive Publication Date: 2017-05-10
HUNAN ZHONGKE AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Second, feed antibiotic pollution
[0005] Third, agricultural antibiotic pollution
[0008] Most of the antibiotic-degrading microorganisms that have been studied at home and abroad are specific lysates, and only play the role of degrading a certain type of antibiotics. The degradation range is limited and the degradation rate is low.

Method used

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  • Mixed bacterial agent capable of degrading antibiotics in soil and preparation method thereof

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Embodiment Construction

[0065] Below in conjunction with embodiment, the present invention will be further described. The following description is by way of example, but the protection scope of the present invention is not limited thereto.

[0066] The mixed bacterial agent of antibiotic in the degradation soil of the present embodiment, its preparation process is as follows:

[0067] (1) Suspension preparation: take antibiotic-contaminated soil, add it to sterilized water at a weight ratio of 1:9, stir to form a suspension, and set aside;

[0068] (2) Colony culture:

[0069] ①. Culture medium preparation: Take 0.1g NaCl, KH 2 PO 4 0.05g, K 2 HPO 4 0.15g, NH 3 NO 3 0.1g, MgSO 4 ·7H 2 O 0.01g, agar 1.5g, mix evenly, add distilled water to 100ml, adjust the pH value to 6.5, then cool to 50°C after autoclaving, then add oxytetracycline to make the final mass concentration 100mg / L, namely Deoxytetracycline basal salt solid medium, spare;

[0070] ②. Cultivation: Dilute the suspension 100 t...

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Abstract

The invention discloses a mixed bacterial agent capable of degrading antibiotics in soil, belonging to the field of microbial technology. The mixed bacterial agent is prepared by mixing Bacillus subtilis J5P2 and Pseudomonas J2 according to a volume ratio of (0.1-3): 1. A preparation method for the mixed bacterial agent comprises the following steps: I, preparation of a suspension; II, colony culture: (1) preparation of a medium and (2) culture; III, separation of strains; IV, subculture and domestication: (1) domestication, (2) preparation of a LB medium, and (3) preservation; V, preparation of inoculum liquid; and VI, preparation of the mixed bacterial agent. The mixed bacterial agent provided by the invention has the characteristics of capacity of effectively degrading a plurality of residual antibiotics in soil and reducing environmental pollution, etc., and can be used for the remediation of land contaminated by antibiotics such as tetracycline, penicillin, sulfadiazines and quinolones.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a mixed microbial agent for degrading antibiotics in soil and a preparation method thereof. Background technique [0002] Antibiotics (tetracyclines, penicillins, sulfadiazines, quinolones, etc.) are chemical substances that can inhibit or affect other biological functions at low concentrations. Its discovery and application have played a huge role in human health care and animal and plant pest control. However, with its large-scale production and application, the pollution problem has become more and more serious. Its abuse can cause antibiotics to generally exist in the environment (especially in the soil environment), causing serious pollution of the soil environment and endangering human health. There are three main sources of antibiotic pollution in my country: [0003] One is the contamination of medical antibiotics. The pollution of medical antibiotics is conside...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/125C12R1/38
CPCC12N1/20B09C1/10
Inventor 文贻荣黄生博
Owner HUNAN ZHONGKE AGRI
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