Mixed bacterial agent capable of degrading antibiotics in soil and preparation method thereof
A technology of mixing bacterial agents and antibiotics, applied in the field of microorganisms, can solve problems such as low degradation rate, limited degradation range, and agricultural antibiotic pollution.
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[0065] Below in conjunction with embodiment, the present invention will be further described. The following description is by way of example, but the protection scope of the present invention is not limited thereto.
[0066] The mixed bacterial agent of antibiotic in the degradation soil of the present embodiment, its preparation process is as follows:
[0067] (1) Suspension preparation: take antibiotic-contaminated soil, add it to sterilized water at a weight ratio of 1:9, stir to form a suspension, and set aside;
[0068] (2) Colony culture:
[0069] ①. Culture medium preparation: Take 0.1g NaCl, KH 2 PO 4 0.05g, K 2 HPO 4 0.15g, NH 3 NO 3 0.1g, MgSO 4 ·7H 2 O 0.01g, agar 1.5g, mix evenly, add distilled water to 100ml, adjust the pH value to 6.5, then cool to 50°C after autoclaving, then add oxytetracycline to make the final mass concentration 100mg / L, namely Deoxytetracycline basal salt solid medium, spare;
[0070] ②. Cultivation: Dilute the suspension 100 t...
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