Diluent for storing the sperm of pig under normal temperature and preparation and dilution method thereof

Abstract

The invention relates to a diluent for storing the sperm of a pig under normal temperature, which is characterized by comprises the following components by weight ratio: 9-11 of glucose, 0.2-0.3 of ethylene diamine tetraacetic acid, 0.5-0.6 of trisodium citrate, 0.08-0.12 of 1570U / mg penicillin and 0.2-0.25 of 720U / mg streptomycin. The diluent is completely dissolved in distilled water with distilled water according to the dilution ratio that diluent of 1g is diluted with distilled water of 20ml, and dilution liquid with the pH of being 6.5-6.8 and the osmotic pressure of being 290-310mOsmo / L is obtained; the diluent of the sperm of the pig, produced by the formulation, can effectively prolong the holding time, has convenient use and low cost, is suitable for being used by an artificial insemination station of the pig and a large-scale pig farm, has good application effect in the long-term production, has wider application range than the imported sperm, and can lead the conception rate of a sow in the oestrous period when being applied to a foreign pig to enable conception rate of a sow to be up to 86.8 percent with the litter size of 11.2 heads.

Description

technical field [0001] The invention relates to a diluent for storing pig semen at room temperature, which is used for artificial insemination of pigs and belongs to the technical field of animal reproduction. Background technique [0002] During natural mating, a boar can only breed one sow at a time, and the boar semen collected at one time is diluted for artificial insemination. Usually, 20-30 sows can be mated, and the pregnancy rate of sows will not decrease. Scientific research has proved that each time an estrous sow is inseminated, the input semen contains 2-3 billion motile sperm, which can ensure the pregnancy rate of the sow. According to the latest scientific research, boar semen quality inspection, sperm moving straight ahead accounted for between 60% and 95% of the total sperm count, and there was no significant difference in the pregnancy rate of sows receiving artificial insemination. According to the commonly used method for evaluating sperm motility in th...

AI Technical Summary

Problems solved by technology

With the rapid development of large-scale and moderate-scale pig farms, modern artificial insemination technology for pigs has begun to be widely promoted. However, due to the lack of high-quality semen diluents in the domestic market, and the semen diluents imported from abroad are expensive, it is difficult to popularize them. , and the currently commonly used diluents all contain yolk, and the yolk is easy to carry pathogenic bacteria, which can easily lead to low pregnancy rate or litter rate

Limit the popularization of artificial insemination technology

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