43results about How to "To achieve the purpose of quantitative detection" patented technology

The invention discloses an N-terminal brain natriuretic peptide precursor detection kit and a detection method thereof. The kit comprises: reagents I: a phosphate buffer solution, a preservative, Tween 20 and polyethylene glycol; reagents II: a phosphate buffer solution, disodium hydrogen phosphate, sodium dihydrogen phosphate of 2 to 5 g / L, sodium chloride, a preservative and sensitized latex particles of a goat anti-human N-terminal-front B type natriuretic peptide antibody; and a calibration product: a phosphate buffer solution, bovine serum albumin, sodium chloride, a preservative and N-terminal-front B type natriuretic peptide antigen. According to the method, the test sensitivity is improved by applying a latex immunoturbidimetric method, the sensitized latex particles suitable for detecting an N-terminal front B type natriuretic peptide precursor are found, and the detection sensitivity is improved; large-batch samples can be detected quantitatively and simultaneously; and the kit comprises the calibration products and the quantitative detection aim is fulfilled.

The invention relates to a medical immunodetection method and particularly relates to a method for using quantum-dot labeled immunochromatography test paper to detect treponema pallidum by an immunological method. The quantum-dot labeled immunochromatography test paper is characterized in that a plastic board is stuck with a glass cellulose membrane A, a glass cellulose membrane B of a quantum-dot labeled treponema pallidum IgG monoclonal antibody, a nitrocellulose membrane and water absorbing paper from bottom to top in sequence, wherein one end of the nitrocellulose membrane is provided with treponema pallidum polyclonal antibody and rabbit antimouse second antibody so as to form a detecting band T and a quality control band C; the quantum-dot labeled treponema pallidum IgG monoclonal antibody is positioned at one end of the glass cellulose membrane and corresponds to the detecting band T and the quality control band C, and the quantum-dot labeled treponema pallidum IgG monoclonal antibody is positioned at one end of a sampling point. The method has the advantages that high specificity of immunoreactions and the fluorescence characteristic of quantum dots are combined, so that the detection sensitivity is about 1000 times higher than that of the current commonly-used colloidal gold detection method.

The invention belongs to the technical fields of novel functional materials, immunoassay and biosensing and provides a preparation method and application of an immunosensor based on titanium dioxide-doped graphene-loaded trepan-shaped gold palladium core-shell nanoparticles. A marker-free electrochemical immunosensor is constructed by taking the titanium dioxide-doped graphene-loaded trepan-shapedgold palladium core-shell nanoparticles as a signal amplifying platform, so that the quantitative determination of ovarian cancer markers is realized, and the immunosensor has the advantages of strong specificity, high sensitivity, low detection limit and the like. The specificity has important scientific significances and application values in the detection of ovary diseases.

The invention relates to a medimmune inspection method, and particularly relates to quantum dot-labeled immunochromatographic test paper, and a method for detecting poliovirus by adopting an immunological method. According to the quantum dot-labeled immunochromatographic test paper, a glass cellulose membrane A, a quantum dot-labeled poliovirus IgG monoclonal antibody glass cellulose membrane B, a cellulose nitrate membrane and absorbent paper are sequentially are bonded on a plastic board from bottom to top, wherein a poliovirus polyclonal antibody and a rabbit-anti-mouse secondary antibody are on one end of the cellulose nitrate membrane so as to form an inspection strip T and a quality control strip C; a quantum dot-labeled poliovirus IgG monoclonal antibody is located at one end of the glass cellulose membrane B and corresponds to the inspection strip T and the quality control strip C, and the quantum dot-labeled poliovirus IgG monoclonal antibody is located at one end of a sample feeding point. The inspection sensitivity of the method is higher than of the currently used method by about 1000 times.

The invention discloses a method for detecting the bending strength of a battery. The method comprises the following steps: firstly, supporting a battery to be detected by two supporting points with the span of L; then applying a load P to a middle position between the two supporting points along the direction which is vertical to the surface of the battery to be detected; recording the size P1 of the load P when the battery to be detected has the deformation with the route a or recording the size P2 of the load P when the battery is damaged; and finally, substituting obtained data into a formula to calculate the value of the bending strength to realize the aim of quantitatively detecting the bending strength of the battery. With the adoption of the method disclosed by the invention, a specific numerical value for evaluating the bending strength of the battery can be obtained; a detection result is objective and the different detection results have the comparability. The invention further provides a device for detecting the bending strength of the battery.

The invention discloses a quantitative detector for muscae volitantes. The quantitative detector is a metal square box device forming an all-closed visual space. Milk white PC plastic is arranged on the inner wall of the square box device, and a lower jaw carrier is mounted at the lower end of the square box device and is connected to a table top through a left stand column and a right stand column. A left eye endoscopy lens and a right eye endoscopy lens are mounted on the outer side of the front end of the square box device. A movable shielding piece is mounted on each of the left eye endoscopy lens and the right eye endoscopy lens. Four inner visual space supplement light sources are mounted at four inner corners of the front end of the square box device. A liquid crystal display screen is mounted at the rear end of the square box device and is connected with a computer of a doctor. The liquid crystal display screen and a screen of the computer of the doctor can sequentially display eight muscae volitantes quantitative detection template pages synchronously. The quantitative detector is simple in structure, convenient to operate and capable of detecting muscae volitantes of patients quantitatively; the muscae volitantes of the patients can be discovered by the doctor in an outpatient department timely, and the objectives of early discovery and early treatment are achieved; the doctor in the outpatient department can acquire accurate and clear quantitative cognition of muscae volitantes symptoms of the patients, and make a quantitative detection report for the muscae volitantes.

The invention discloses an electrochemical biosensor for detecting bisphenol-A, and a preparation method and an application thereof. The electrochemical biosensor comprises a counter electrode, a reference electrode and a working electrode. The electrochemical biosensor is characterized in that the working electrode is a glassy carbon electrode surface-modified by a graphene / gold complex, tyrosinase and chitosan. The preparation method comprises a step of preparing the graphene oxide; a step of preparing the graphene / gold complex; and a step of polishing and cleaning a bare glassy carbon electrode. A three electrode system electrochemical enzyme sensor is formed in the way that the graphene / gold complex, the tyrosinase and the chitosan are modified on the glassy carbon electrode to be used as the working electrode, a platinum electrode is used as the counter electrode and a saturated calomel electrode is used as the reference electrode. The concentration of bisphenol-A in a to-be-detected sample is determined by immersing the electrochemical biosensor in the to-be-detected sample and according to a quantitative relationship between corresponding current value and the concentration of bisphenol-A. The electrochemical biosensor has high sensitivity, strong selectivity, high accuracy and fast detection speed. The preparation method is simple and practical, and convenient for operations in actual detection.

The invention relates to a medical immunodetection method and in particular relates to a method for detecting a Japanese encephalitis virus (JEV) by an immunological method by using a quantum dot labelled immunochromatographic test strip. The quantum dot labelled immunochromatographic test strip is characterized in that a glass cellulose membrane A, a glass cellulose membrane B of a quantum dot labelled JEV IgG (immunoglobulin G) monoclonal antibody, a nitrocellulose membrane and absorbent paper are stuck to a plastic board from bottom to top in sequence, wherein one end of the nitrocellulose membrane has a JEV polyclonal antibody and a rabbit anti-mouse second antibody, thereby forming a detection zone T and a quality control zone C; the quantum dot labelled JEV IgG monoclonal antibody is arranged at the other end of the glass cellulose membrane B, corresponds to the detection zone T and the quality control zone C and is arranged at one end of a sampling point. The detection sensitivity of the method is about 1000 times higher than that of the detection method frequently used at present.

The invention provides a method for detecting vibrio parahemolyticus by an AU-PDMS composite membrane on the basis of aptamer identification. A specific recognition vibrio parahemolyticus aptamer is fixed on the surface of nanogold, a Raman signal module is modified as a signal probe, the specific recognition vibrio parahemolyticus is fixed on an APT-AU-PDMS composite membrane formed by a charge effect to serve as a detection substrate, and a 'sandwich' structure detection sensor is established. The lowest detection limit of the method for the vibrio parahemolyticus can be 12cfu / mL, in addition, the method can be applied to the detection of the vibrio parahemolyticus in shrimp samples, and a result is accurate and reliable.

The invention discloses a N-terminal brain natriuretic peptide precursor detection kit and a detection method thereof; the kit includes: reagent one: phosphate buffer, preservative, Tween 20, polyethylene glycol 6000; reagent two: Phosphate buffer, disodium hydrogen phosphate, 2-5g / L sodium dihydrogen phosphate, sodium chloride, preservatives, sensitized latex particles of goat anti-human N-terminal-pre-B type natriuretic peptide antibody; calibrator: phosphoric acid Saline buffer solution, bovine serum albumin, sodium chloride, preservatives, N-terminal-pro-B-type natriuretic peptide antigen; method The latex immunoturbidimetric method was used to improve the sensitivity of the test, and to find a suitable detection method for the N-terminal pro-BNP Sensitized latex particles to improve detection sensitivity; through different automatic biochemical analyzers with different reagent-sample ratios, a large number of samples can be quantitatively detected at the same time; the kit contains calibrators to achieve the purpose of quantitative detection.

The invention relates to a medical immunodetection method and particularly relates to a method for using quantum-dot labeled immunochromatography test paper to detect mumps virus by an immunological method. The quantum-dot labeled immunochromatography test paper is characterized in that a plastic board is stuck with a glass cellulose membrane A, a glass cellulose membrane B of a quantum-dot labeled mumps virus IgG monoclonal antibody, a nitrocellulose membrane and water absorbing paper from bottom to top in sequence, wherein one end of the nitrocellulose membrane is provided with mumps virus polyclonal antibody and rabbit antimouse second antibody so as to form a detecting band T and a quality control band C; the quantum-dot labeled mumps virus IgG monoclonal antibody is positioned at one end of the glass cellulose membrane and corresponds to the detecting band T and the quality control band C, and the quantum-dot labeled mumps virus IgG monoclonal antibody is positioned at one end of a sampling point. The method has the advantage that the detection sensitivity is about 1000 times higher than that of the commonly-used method.

The invention belongs to the technical field of safety protection, and discloses a gas auxiliary detection device. The gas auxiliary detection device comprises a support and a tank body; the tank bodyis fixedly arranged on the support, and is provided with an internal cavity and a gas inlet rod; the gas inlet rod is provided with an annular chamber; the annular chamber is provided with a gas inlet; a high pressure gas tank is fixedly arranged on the support; the two side walls of the internal cavity are provided with blocking plates and air bags; a first chain wheel is fixedly arranged on a rotating shaft; the bottom of each blocking plate is provided with an infrared lamp; the bottom of the internal cavity is provided with an opening and a detection box; the detection box is provided with a first glass plate; a photoresistor is arranged in the detection box; the photoresistor is connected with an indicating lamp through electric connection; the support is provided with internally meshed ratchet wheel structure; the internally meshed ratchet wheel structure comprises an outer ratchet wheel, a driving ratchet wheel, a non-return ratchet wheel, and a driving wheel; a second chain wheel is fixedly arranged in the driving wheel; the outer rim of the outer ratchet wheel is fixedly provided with a rotating disc; the rotating disc is provided with a second glass plate; the second glass plate is provided with a transparent adhesive tape; and the adhesive surface of the transparent adhesive tape is arranged to be toward the internal cavity. The gas auxiliary detection device is simple in structure, and is capable of realizing air detection without filtering particles.

The invention relates to a medical immunodetection method, and particularly relates to a method for detecting hepatitis c virus by quantum-dot-marked immunochromatographic test paper through immunology. According to the quantum-dot-marked immunochromatographic test paper, a glass cellulose membrane A, a glass cellulose membrane B of a quantum-dot-marked hepatitis c virus monoclonal antibody, a nitrocellulose membrane and absorbent paper are sequentially adhered to a plastic plate from top to bottom, wherein one end of the nitrocellulose membrane is provided with a hepatitis c virus polyclonal antibody and a rabbit antimouse second antibody to form a detection band T and a detection band C; the quantum-dot-marked hepatitis c virus monoclonal antibody is located at one end of the glass cellulose membrane B and corresponds to the detection band T and the detection band C, and the quantum-dot-marked hepatitis c virus monoclonal antibody is located at the end of a sampling point. The sensitiveness of the method is about 1000 times higher than that of a colloidal gold method.

The invention relates to a medimmune inspection method, and particularly relates to quantum dot-labeled immunochromatographic test paper, and a method for detecting rubella virus by adopting an immunological method. According to the quantum dot-labeled immunochromatographic test paper, a glass cellulose membrane A, a quantum dot-labeled rubella virus IgG monoclonal antibody glass cellulose membrane B, a cellulose nitrate membrane and absorbent paper are sequentially bonded on a plastic board from bottom to top, wherein a rubella virus polyclonal antibody and a rabbit-anti-mouse secondary antibody are arranged on one end of the cellulose nitrate membrane so as to form an inspection strip T and a quality control strip C; a quantum dot-labeled rubella virus IgG monoclonal antibody is located at the other end of the glass cellulose membrane B and corresponds to the inspection strip T and the quality control strip C, and the quantum dot-labeled rubella virus IgG monoclonal antibody is located at one end of a sample feeding point. The inspection sensitivity of the method is higher than of the currently used method by about 1000 times.

The invention belongs to the technical fields of new functional materials, immune analysis and biosensing, and provides a preparation method and application of a titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticle immune sensor. Titanium dioxide-doped graphene-loaded sea cucumber-like gold-palladium core-shell nanoparticles were used as a signal amplification platform to construct a label-free electrochemical immunosensor, which realized the quantitative detection of ovarian cancer markers, with strong specificity, high sensitivity, and detection Low limit and other advantages. The detection of ovarian diseases has important scientific significance and application value.

The invention discloses an LAMP (loop-mediated isothermal amplification) method for detecting the florfenicol resistance of bacteria through a series of research work of preparing materials, designing and synthesizing LAMP primers, extracting bacterial plasmid DNA (deoxyribonucleic acid), optimizing an LAMP reaction system and the like, and a corresponding LAMP primer group including an outer primer pair F3 and B3 and an inner primer pair FIP and BIP is designed according to sequences of florfenicol-resistant floR genes of the bacteria. For an unknown sample, the florfenicol resistance of a bacterial sample can be judged by detecting the value of the time when the turbidity value of floR gene amplification is 0.1 only. Specific and sensitive detection results prove that the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by the method is high in sensitivity which is 100 times that of the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by a conventional PCR method, the reaction can be monitored in real time, the copy number of the floR genes can be quantitatively detected, the detection results can be quickly and accurately obtained, and the convenience is brought to quick detection of the florfenicol resistance of the bacteria.