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Electrochemical biosensor for detecting bisphenol-A, and preparation method and application thereof

A biosensor, electrochemical technology, applied in the direction of material electrochemical variables, etc., can solve the problems of limiting the development of high-sensitivity detection technology, undisclosed bisphenol A electrochemical biosensor, large van der Waals force, etc., to achieve good production reproducibility , Improve electronic conductivity, enhance the effect of sensitivity

Inactive Publication Date: 2015-04-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a large van der Waals force (5.9kJ mol-1) between graphene sheets, which is prone to accumulation and aggregation, which limits the development of high-sensitivity detection technology to a certain extent.
At present, there are no relevant research reports on the preparation of electrochemical biosensors for detecting the concentration of bisphenol A by using graphene at home and abroad.

Method used

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  • Electrochemical biosensor for detecting bisphenol-A, and preparation method and application thereof
  • Electrochemical biosensor for detecting bisphenol-A, and preparation method and application thereof
  • Electrochemical biosensor for detecting bisphenol-A, and preparation method and application thereof

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specific Embodiment 1

[0022] An electrochemical biosensor for detecting the concentration of bisphenol A, the electrochemical sensor is a three-electrode system, wherein the counter electrode is a platinum electrode, the reference electrode is a saturated calomel electrode, and the working electrode is a graphene / gold - Tyrosinase-chitosan modified glassy carbon electrode (GR / Au-Tyr-CS / GCE).

specific Embodiment 2

[0023] A preparation method for an electrochemical biosensor for detecting bisphenol A, the specific steps are as follows:

[0024] (1) Preparation of graphene oxide

[0025] Mix potassium permanganate and flake graphite evenly at a mass ratio of 6:1 to obtain a mixed powder, mix concentrated sulfuric acid and phosphoric acid at a volume ratio of 9:1 to obtain a mixed solution, and mix the mixed powder and the mixed solution at a mass-volume ratio of 21g: 400ml Mix, then stir at 45-55°C for 10-14h, then cool to room temperature, add 3 / 400 mixed solution volume with a mass fraction of 30% H 2 o 2 Solution, after fully reacting to room temperature, use non-woven fabric suction filtration, the filtrate obtained after suction filtration is centrifuged at 4000rpm for 4h, the floating layer is poured off, and the solid matter obtained by centrifugation is sequentially used with equal amounts of deionized water, the mass fraction is 30% HCl solution, washed with ethanol, after e...

specific Embodiment 3

[0033] A method for the detection of bisphenol A with an electrochemical enzyme sensor based on graphene / gold complex-tyrosinase-chitosan (GR / Au-Tyr-CS)

[0034] The graphene / gold-tyrosinase-chitosan (GR / Au-Tyr-CS) mixture prepared in the above specific example 2 was modified onto the surface of the bare glassy carbon electrode, and the enzyme load was fixed on the surface of the glassy carbon electrode by adsorption. On the electrode surface, a platinum electrode is used as a counter electrode, and a saturated calomel electrode is used as a reference electrode to form an electrochemical enzyme sensor of a three-electrode system, and the enzyme sensor is composed. When the target analyte bisphenol A exists in the detection environment, an irreversible oxidation-reduction reaction occurs between the target and the tyrosinase immobilized on the electrode surface. As the concentration of the target increases, the response current also increases. The phenomenon detects the bisphen...

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Abstract

The invention discloses an electrochemical biosensor for detecting bisphenol-A, and a preparation method and an application thereof. The electrochemical biosensor comprises a counter electrode, a reference electrode and a working electrode. The electrochemical biosensor is characterized in that the working electrode is a glassy carbon electrode surface-modified by a graphene / gold complex, tyrosinase and chitosan. The preparation method comprises a step of preparing the graphene oxide; a step of preparing the graphene / gold complex; and a step of polishing and cleaning a bare glassy carbon electrode. A three electrode system electrochemical enzyme sensor is formed in the way that the graphene / gold complex, the tyrosinase and the chitosan are modified on the glassy carbon electrode to be used as the working electrode, a platinum electrode is used as the counter electrode and a saturated calomel electrode is used as the reference electrode. The concentration of bisphenol-A in a to-be-detected sample is determined by immersing the electrochemical biosensor in the to-be-detected sample and according to a quantitative relationship between corresponding current value and the concentration of bisphenol-A. The electrochemical biosensor has high sensitivity, strong selectivity, high accuracy and fast detection speed. The preparation method is simple and practical, and convenient for operations in actual detection.

Description

technical field [0001] The invention relates to a detection method of bisphenol A, in particular to an electrochemical biosensor based on a graphene / gold composite nanomaterial for detection of bisphenol A and a preparation method thereof. Background technique [0002] Bisphenol A, namely 4, 4-dihydroxydiphenylpropane (BPA for short), is an environmental endocrine disrupting chemical (endorsing disrupting chemicals, EDCs) with estrogen activity, which causes serious pollution and harm to the ecological environment. Bisphenol A accumulates in the human body through the food chain, causing various diseases in humans and animals, can lead to the distortion of human and animal germ cells and the reduction of reproductive rate, and can cause the feminization of gastropod male individuals. At present, the detection methods of bisphenol A mainly focus on gas chromatography, gas chromatography-mass spectrometry, liquid chromatography, liquid chromatography-mass spectrometry, etc. Al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/38
Inventor 潘道东顾愿愿曾小群曹锦轩李桦朱浩嘉李建龙
Owner NINGBO UNIV
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