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Preparation method and application of an immunosensor based on titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles

An immunosensor and aminated graphene technology, applied in instruments, scientific instruments, measuring devices, etc., can solve the problems of difficult early diagnosis, atypical early symptoms, missed diagnosis and misdiagnosis, etc., to improve sensitivity, increase specific surface area, detect quick effect

Inactive Publication Date: 2020-01-21
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the atypical early symptoms of ovarian cancer clinical symptoms, it is very easy to cause missed diagnosis and misdiagnosis, which is a big problem in early diagnosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1. A kind of preparation method based on the immunosensor of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, the operation steps are as follows:

[0038] (1) The glassy carbon electrode with a diameter of 4.0 mm was polished into a mirror surface with 1.0 µm, 0.3 µm and 0.05 µm alumina polishing powder in sequence, and cleaned ultrasonically in absolute ethanol;

[0039] (2) Take 6.0 μL, 1.0 mg / mL of titanium dioxide-doped graphene-loaded sea cucumber-like gold-palladium core-shell nanoparticles dispersion and apply it to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0040] (3) Continue to drop 6.0 µL, 10.0 µg / mL human epididymis protein 4 antibody onto the surface of the electrode, and let it dry in a refrigerator at 4 °C;

[0041] (4) Continue to add 3.0 µL, 1.0 wt% bovine serum albumin solution dropwise to the electrode surface to seal t...

Embodiment 2

[0043] Embodiment 2. A kind of preparation method based on the immunosensor of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, the operation steps are as follows:

[0044] (1) The glassy carbon electrode with a diameter of 4.0 mm was polished into a mirror surface with 1.0 µm, 0.3 µm and 0.05 µm alumina polishing powder in sequence, and cleaned ultrasonically in absolute ethanol;

[0045] (2) Take 6.0 μL, 2.0 mg / mL of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles dispersion and apply it to the surface of the electrode, dry it at room temperature, rinse the surface of the electrode with ultrapure water, and dry it;

[0046] (3) Continue to drop 6.0 µL, 10.0 µg / mL human epididymis protein 4 antibody onto the surface of the electrode, and let it dry in a refrigerator at 4 °C;

[0047] (4) Continue to add 3.0 µL, 1.0 wt% bovine serum albumin solution dropwise to the electrode s...

Embodiment 3

[0049] Embodiment 3. A kind of preparation method based on the immunosensor of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, the operation steps are as follows:

[0050] (1) The glassy carbon electrode with a diameter of 4.0 mm was polished into a mirror surface with 1.0 µm, 0.3 µm and 0.05 µm alumina polishing powder in sequence, and cleaned ultrasonically in absolute ethanol;

[0051] (2) Take 6.0 μL, 3.0 mg / mL of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles dispersion and apply it to the surface of the electrode, dry it at room temperature, rinse the surface of the electrode with ultrapure water, and dry it;

[0052] (3) Continue to drop 6.0 µL, 10.0 µg / mL human epididymis protein 4 antibody onto the surface of the electrode, and let it dry in a refrigerator at 4 °C;

[0053] (4) Continue to add 3.0 µL, 1.0 wt% bovine serum albumin solution dropwise to the electrode s...

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Abstract

The invention belongs to the technical fields of new functional materials, immune analysis and biosensing, and provides a preparation method and application of a titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticle immune sensor. Titanium dioxide-doped graphene-loaded sea cucumber-like gold-palladium core-shell nanoparticles were used as a signal amplification platform to construct a label-free electrochemical immunosensor, which realized the quantitative detection of ovarian cancer markers, with strong specificity, high sensitivity, and detection Low limit and other advantages. The detection of ovarian diseases has important scientific significance and application value.

Description

technical field [0001] The invention belongs to the technical fields of novel nanomaterials, immune analysis and biosensing, and provides a method for preparing an immunosensor based on titania-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, and an electrochemical sensor constructed by the method. Application of immunosensor in detection of human epididymis protein 4 antigen. Background technique [0002] Ovarian cancer is a common malignant tumor in gynecology, and its mortality rate ranks first in gynecological malignant tumors. Therefore, timely screening and detection in the early stage of ovarian cancer is an important way to reduce mortality. However, due to the atypical early symptoms of ovarian cancer clinical symptoms, it is very easy to cause missed diagnosis and misdiagnosis, which is a big problem in early diagnosis. Therefore, it is very important to develop rapid, simple and sensitive methods for detecting ovarian cancer mar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N33/68G01N33/574
CPCG01N27/308G01N27/3275G01N33/57449G01N33/57484G01N33/689
Inventor 刘青颜芹刘会谭召灵董慧董云会
Owner SHANDONG UNIV OF TECH
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