Detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase

Abstract

The invention discloses a detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase. The detecting card comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a fluorescent pad, a nitrocellulose membrane, a detecting line, a quality control line, water absorption filter paper, sample feeding ports, sample adding tubes, a support, a stop piece, a sample adding zone, a reaction zone, a sample flowing pad, a bulge, a reaction film and an added sample filtering zone, wherein the sample pad, the nitrocellulose membrane, the fluorescent pad, the water absorption filter paper, the added sample filtering zone, the sample flowing pad, the bulge, the sample adding zone and the reaction zone are arranged on the PVC bottom plate; one end of the sample pad is overlapped on the fluorescent pad, the other end of the fluorescent pad is overlapped on the nitrocellulose membrane, the water absorption filter paper is overlapped at the other end of the nitrocellulose membrane, the detecting line and the quality control line are formed on the nitrocellulose membrane, the added sample filtering zone and the sample flowing pad are contacted, and the sample adding tubes are arranged above the sample pad, the added sample filtering zone and the sample adding zone. The detecting card has the advantages of simplicity, convenience and rapidness in operation and the like.

Description

technical field [0001] The invention relates to a detection card for bone-derived alkaline phosphatase, hemoglobin and alanine aminotransferase, which is applied in the field of medical in vitro diagnostic reagents. Background technique [0002] Bone-derived alkaline phosphatase (BALP) is a subtype of alkaline phosphatase isoenzyme, which is synthesized by osteoblasts and mainly concentrated in ossification sites. Bone-derived alkaline phosphatase is synthesized by osteoblasts Other tissues and organs do not synthesize BALP, which is unique to osteogenic tissue. BALP activity is directly proportional to the number of active osteoblasts, and is a specific marker reflecting the activity and number of osteoblasts. Therefore, its activity directly, specifically, and sensitively reflects the dynamic information of bone formation and resorption. It is a tissue-specific biochemical marker that covers the entire course of the disease and is not affected by other conditions. In...

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Problems solved by technology

When the ion difference between the inside and outside of liver cells changes, the cell membrane swells, the cell pores increase or the cells die, alanine aminotransferase can be released from the cells into the blood, resulting in a significant increase in serum or plasma alanine aminotransferase, so alanine aminotransferase is recognized by the World Health Organization It is recommended as the most sensitive detection index for liver function damage. At present, the clinical detection of alanine aminotransferase is mainly the method of using liquid reagents of biochemical analyzers. This method is time-consuming, complicated to operate, and requires relatively large instruments.

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