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Substrate regional band resolution chemical luminescent multi-component immunity analytical method and detection system thereof

A detection system, chemiluminescence technology, applied in the direction of chemiluminescence/bioluminescence, analysis by causing chemical reaction of materials, analysis of materials, etc., can solve problems such as expensive instruments and supporting kits, and financially insufficient institutions cannot afford it , to achieve the effect of saving coating antibody, less manual operation, and simple equipment

Inactive Publication Date: 2007-02-07
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of the instrument and supporting kits is very expensive, and it is difficult for institutions with insufficient financial resources to afford it.
Moreover, the instrument is also in a single-component analysis mode, and multiple parallel determinations are still required for complex samples

Method used

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  • Substrate regional band resolution chemical luminescent multi-component immunity analytical method and detection system thereof
  • Substrate regional band resolution chemical luminescent multi-component immunity analytical method and detection system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 is combined with attached figure 1 Further description of the flow-through two-component chemiluminescence immunoassay detection system:

[0041] The flow-through substrate zone resolution multi-component chemiluminescence immunoassay detection system includes a solution delivery system, an immunoreactor, a chemiluminescence detector and a computer. The solution transmission system consists of a multi-channel peristaltic pump 6, a PTFE connecting pipe 7 with an inner diameter of 0.8mm and a multi-position selector valve 8. The five connecting pipes 7 are respectively connected to the multi-position selector valve through the multi-channel peristaltic pump 6. The five valve positions of valve 8 are ports 1, 2, 4, and 5. Inlet 1 transfers sample S, inlet 5 transfers regeneration buffer RB, inlet 4 transfers washing buffer WB, and inlets 3 and 2 transfer two chemiluminescent substrates respectively S 1 and S 2 One end of each connecting pipe 7 is connected ...

Embodiment 2

[0042] Embodiment 2 chemiluminescence and component immunoassay method:

[0043] Table 1 Specific analysis process

Embodiment 3

[0045] Taking two important tumor markers: carcinoembryonic antigen (CEA) and cancer antigen 125 (CA 125) as examples, the application of the flow-type two-component chemiluminescence immunoassay system is illustrated.

[0046] The immunoreactor is an aldehyde-activated UltraBind membrane coated with mouse monoclonal anti-CEA and mouse monoclonal anti-CA 125. The remaining active sites are blocked with bovine serum albumin and fixed in a 50 microliter flow cell. The tracer antibody to CEA was mouse monoclonal anti-CEA labeled with alkaline phosphatase, and the tracer antibody to CA 125 was mouse monoclonal anti-CA 125 labeled with horseradish peroxidase.

[0047] As shown in Table 1, the valve is switched to position 1, and the mixture of 25 microliters of sample, 12.5 microliters of CEA tracer antibody and 12.5 microliters of CA 125 tracer antibody is passed into the flow cell where the immunoreactor is fixed, Incubate statically for 20 minutes. Then switch the valve positio...

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Abstract

The multi-constituent immunity analysis and detection system comprises: a solution transmission system with a multi-channel peristaltic pump (6) and a connection pipe (7) and a multi-position selection valve (8), an immunity reactor, a chemiluminescence detector, and a computer, wherein the analysis method comprises: fixing the packed antibody in the reactor, adding sample and enzyme-mark antibody; marking with HRP and alkaline phosphates to form two types of sandwich composites; clearing, crossing in turns the HRP chemiluminescence substrate area, cleaning buffer liquid area and the alkaline phosphates chemiluminescence substrate area. This invention has fast speed and high sensitivity for wide application.

Description

1. Technical field [0001] The invention is a substrate zone resolution chemiluminescence technique, which relates to a simultaneous immunoassay method for multiple components in the same sample, especially a multi-component immunoassay method combined with chemiluminescence and flow analysis techniques; Detection system for two-component chemiluminescent immunoassays. 2. Background technology [0002] As a highly selective and sensitive analytical method, immunoassay has been widely used in clinical diagnosis, environmental monitoring, food safety and other fields. In the field of practical application, it is often necessary to determine the content of multiple components in complex samples. For example, in tumor diagnosis, the joint analysis of multiple tumor markers provides a strong basis for diagnosis. [0003] At present, in order to realize the combined analysis of multiple components in complex samples, the mode of multiple parallel single-component analysis is often...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/76G01N35/08
Inventor 鞠熀先付志锋
Owner NANJING UNIV
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