Magnetic separation enzyme-linked immune detecting method for thyroid gland peroxidase antibody

An enzyme-linked immunoassay and peroxidase technology, which is applied in biological testing, measuring devices, and analytical materials, can solve problems such as the lack of clear research on basic biological properties, and achieve the effect of improving sensitivity and accuracy

Inactive Publication Date: 2005-03-16
北京倍爱康生物技术有限公司
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Problems solved by technology

[0002] As we all know, the serum of patients with thyroid autoimmune diseases usually contains thyroid microsomal antigen. Since the discovery of this antigen more than 20 years ago, its basic biological properties have not been clearly studied.

Method used

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Embodiment Construction

[0027] Materials and Instruments

[0028] 1. Standard 0, 10, 40, 100, 250, 500IU / ml (refer to WHO 1 ST IRP 66 / 387 standard) each 1ml.

[0029] 2. Magnetic separation reagent (10mg / ml) 20ml.

[0030] 3. Secondary antibody enzyme-labeled reagent (1μg / ml) 20ml.

[0031] 4. Washing liquid 100ml;

[0032] 5. 100ml of substrate solution.

[0033] 6. Stop solution 200ml.

[0034] 7. Sample diluent 100ml.

[0035] 8. 100 serum samples from patients with thyroid autoimmune disease and 100 serum samples from normal people.

[0036] 9. Magnetic separation enzyme-linked immunoassay instrument type I (produced by Beijing Beiaikang Biotechnology Co., Ltd.).

[0037] 10. Water bath (for 37°C warm bath).

[0038] Reagent preparation:

[0039] The preparation of the above-mentioned solutions 1-6 is carried out with reference to the preparation process described in the method of the present invention.

[0040] The dilution solution is prepared as follows: add 0.2% sodium azide to cal...

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Abstract

This invention provides a human hypothyroid peroxid enzyme antibody magnetic separation enzyme immune measurement method, whose steps are the following: to add sample to react with immune; to wash and to join two anti-enzyme indicate reagent and to wash again; to add monophosphatase fenolftaleina substrate solvent; to terminate color forming and to read absorbing value. The advantages in this invention are the following: it uses reformed human hypothyroid peroxid enzyme antigen fold immune magnetism micro ball as fixed phase and uses alkalescence phosphate enzyme as mark enzyme. Through the quantitative measurement of peroxid enzyme antigen of the hypothyroid patient and normal serum and other clinic symptom, it can judge whether the patient has got the self-immune hypothyroid illness.

Description

[0001] The invention relates to the field of immunoassay and analysis, in particular to an enzyme-linked immunoassay method for magnetic separation of human thyroid peroxidase antibody. Background technique [0002] As we all know, the serum of patients with thyroid autoimmune diseases usually contains thyroid microsomal antigen. Since the discovery of this antigen more than 20 years ago, its basic biological properties have not been clearly studied. With the development of molecular biology techniques, many studies have confirmed that the main component of thyroid microsomal antigen is thyroid peroxidase (NAOKATA YOKOYAMA et al., Journal of clinical Endocrinology and Metabolism). Thyroid peroxidase is a membrane-integrated protein located on the apical membrane of follicular cells. The enzyme uses heme as a prosthetic group with a molecular weight of about 101KD. The traditional detection of thyroid autoimmune diseases is usually based on thyroid microsomal antibodies, radioi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N33/551
Inventor 刘振世周昊陈海生张玉庆杨祥良
Owner 北京倍爱康生物技术有限公司
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