Allergenic specific IgE antibody immunoassay detection kit and preparation method thereof
A detection kit and immunoassay technology, applied in the direction of chemical reaction of materials for analysis, analysis of materials, measurement devices, etc., can solve the problems of difficulty in providing sensitivity, low accuracy, long time consumption, etc. Small background interference, high accuracy, easy to use effects
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Embodiment 1
[0036] Example 1: Dust mite allergen-specific IgE antibody immunoassay detection kit
[0037] D. farinae allergen-specific IgE antibody immunoassay kit, including: (1) superparamagnetic microbeads coupled with D. farinae allergen protein, both of which are 100 μg D. farinae allergen protein / mg superparamagnetic microbead (2) superparamagnetic microbeads coupled with mouse anti-human IgE antibody, the two are coupled and mixed at a ratio of 10 μg goat anti-human IgE antibody / mg superparamagnetic microbeads; (3) human IgE antibody standard, derived from human serum, purified by affinity chromatography with a concentration of 100IU / mL (refer to the international reference standard for human IgE published by the World Health Organization); (4) rabbit anti-human IgE antibody labeled with alkaline phosphatase; And (5) alkaline phosphatase chemiluminescence substrate 3-(2′-helixadamantane)-4-methoxy-4-(3″-o-oxyacylphenyl)-1,2-dioxane Butane (AMPPD).
[0038] Wherein, the interior o...
Embodiment 2
[0039] Example 2: Mango allergen-specific IgE antibody immunoassay detection kit
[0040] Mango allergen-specific IgE antibody immunoassay detection kit, including: (1) magnetic microbeads coupled with mango allergen protein, the two are coupled and mixed at a ratio of 300 μg mango allergen protein / mg magnetic microbead; ( 2) Magnetic beads coupled with rabbit anti-human IgE antibody, the two are coupled and mixed at a ratio of 40 μg rabbit anti-human IgE antibody / mg magnetic beads; (3) Human IgE antibody standard, derived from human serum, tested The concentration after purification by affinity chromatography is 100IU / mL (refer to the international reference standard for human IgE published by the World Health Organization); (4) Goat anti-human IgE antibody labeled with alkaline phosphatase; (5) Alkaline phosphatase chemiluminescent substrate 3-(2′-spiroadamantane)-4-methoxy-4-(3″-o-oxyacylphenyl)-1,2-dioxetane (AMPPD); and (6) black plastic Opaque microplates.
[0041] Suc...
Embodiment 3
[0042] Example 3: Honeybee Allergen-Specific IgE Antibody Immunoassay Detection Kit
[0043] Honeybee allergen-specific IgE antibody immunoassay detection kit, including: (1) superparamagnetic microbeads coupled with bee allergen protein, the two are coupled at a ratio of 200 μg bee allergen protein / mg superparamagnetic microbeads (2) Superparamagnetic microbeads coupled with goat anti-human IgE antibody, the two are coupled and mixed at a ratio of 30 μg rabbit anti-human IgE antibody / mg superparamagnetic microbeads; (3) Human IgE antibody standard The product is derived from human serum, and the concentration after purification by affinity chromatography is 100IU / mL (refer to the international reference standard for human IgE published by the World Health Organization); (4) mouse anti-human IgE antibody labeled with alkaline phosphatase; (5) Alkaline phosphatase chemiluminescence substrate 3-(2′-helicadamantane)-4-methoxy-4-(3″-o-oxyacylphenyl)-1,2-dioxetane (AMPPD ); (6) ce...
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