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Breeding method of polyclonal antibody cold-resistant two-line sterile line

A sterile line, cold-resistant technology, applied in the fields of botanical equipment and methods, applications, plant genetic improvement, etc.

Active Publication Date: 2013-08-07
福建亚丰种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, rice pests and diseases have occurred seriously in my country, with rapid development of resistance and great pressure on prevention and control. There have been large-scale production reductions due to pests and diseases. Although spraying pesticides for prevention and control can achieve certain results, excessive spraying of pesticides not only causes serious grain damage. Pollution and environmental pollution have also increased the burden on farmers and raised food prices
Scientific research has proved that the most efficient, economical and environmentally friendly way to prevent and control rice diseases and insect pests is to breed rice varieties that are resistant to diseases and insect pests.
Especially in the sterile lines of two-line hybrid rice that are popularized and applied in production at present, there are very few varieties with high resistance or multi-resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Select rice stem borer-resistant variety IR19735-30-3-3 (A) (Zhou Zuming. Identification of rice varieties resistant to rice stem borer. Hunan Agricultural Science, 1986(03):19-22), rice blast resistant variety Dacca6 (B) (Zheng Yanmei. Genetic diversity analysis of blast-resistant rice variety Dacca6 and some hybrid rice parents. Journal of Tropical Crops, 2011,32(10):1849-1852), dwarf-resistant variety Ce 64 (C) (Huang Jingxia .Research and Application of Multi-resistance Breeding in Rice in Hunan Province. Hunan Agricultural Science, 1995(06):10-13), Tainan No. 6 (D), a cold-tolerant and resistant source variety (D) (Huang Jingxia. Research on Multi-resistance Breeding in Rice in Hunan Province and application. Hunan Agricultural Science, 1995(06): 10-13), crossed A / B and C / D, got hybrid F 1 Substituted by F 1-AB and F 1-CB express;

[0024] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD ...

Embodiment 2

[0034] 1) Select rice borer-resistant variety IR19735-30-3-3 (A), rice blast-resistant variety Dacca6 (B), dwarf-resistant variety Che 64 (C), cold-resistant source variety Tainan 6 (D) , make cross A / B and C / D, get cross F 1 Substituted by F 1-AB and F 1-CB express;

[0035] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;

[0036] 3) Plant F 1-ABCD , F 2-ABCD , planting F 2-ABCD , the population is more than 2000 plants, and the fields are tested for rice borer, rice blast, dwarf disease and cold tolerance resistance tests respectively, and the plants that are all resistant to 4 kinds of diseases, insects and cold damage are screened and marked, and the mature plants are harvested separately ( f 3-ABCD );

[0037] 4) The strains are planted with F that is fully resistant to 4 kinds of diseases, insects, and cold damage. 3-ABCD , 40 plants were planted for each line, and the resistance ...

Embodiment 3

[0045] 1) Select rice borer-resistant variety IR19735-30-3-3 (A), rice blast-resistant variety Dacca6 (B), dwarf-resistant variety Che 64 (C), cold-resistant source variety Tainan 6 (D) , make cross A / B and C / D, get cross F 1 Substituted by F 1-AB and F 1-CB express;

[0046] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;

[0047] 3) Plant F 1-ABCD , F 2-ABCD , planting F 2-ABCD , the population is more than 2000 plants, and the fields are tested for rice borer, rice blast, dwarf disease and cold tolerance resistance tests respectively, and the plants that are all resistant to 4 kinds of diseases, insects and cold damage are screened and marked, and the mature plants are harvested separately ( f 3-ABCD );

[0048] 4) The strains are planted with F that is fully resistant to 4 kinds of diseases, insects, and cold damage. 3-ABCD , 40 plants were planted for each line, and the resistance ...

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Abstract

The invention discloses a breeding method of a polyclonal antibody cold-resistant two-line sterile line, belonging to the technical field of breeding for disease resistance. According to the breeding method, by using a two-line sterile line as a recurrent parent, and using a tryporyza incertulas-resistant variety, a rice blast-resistant variety, a stunt-resistant variety and a cold-resistant variety as antigen parents, through a hybrid polymerization process, antigen genes of four rice diseases, insect and cold damages are polymerized in the same rice material, and then subjected to multi-generation backcross and screening together with the recurrent parent of the two-line sterile line, so that the insect and cold-resistant two-line sterile line, with economical characters, sterility period translative temperature and sterility highly consistent with those of the recurrent parent of the two-line sterile line, is bred. The two-line sterile line is used for preparing two-line hybrid rice, and thus the level of resisting insects and low-temperature coldness of the hybrid rice can be improved.

Description

technical field [0001] The invention specifically relates to a method for breeding a multi-resistant and cold-tolerant two-line sterile line, and belongs to the technical field of rice resistance breeding. Background technique [0002] As China's largest food crop, rice has made great contributions to solving China's food security and improving people's health. In recent years, rice pests and diseases have occurred seriously in my country, with rapid development of resistance and great pressure on prevention and control. There have been large-scale production reductions due to pests and diseases. Although spraying pesticides for prevention and control can achieve certain results, excessive spraying of pesticides not only causes serious grain damage. Pollution and environmental pollution have also increased the burden on farmers and raised food prices. Scientific research has proved that the most efficient, economical and environmentally friendly way to prevent and control ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 章清杞陈志伟
Owner 福建亚丰种业有限公司
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