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Macropore high-capacity agarose gel media preparing method

An agarose gel, high-capacity technology, used in chemical instruments and methods, measuring devices, material separation, etc., can solve the problems of difficult pore size control, medium performance and use effects, and complex preparation processes, achieving simple equipment and raw materials. Inexpensive, biocompatible effect

Inactive Publication Date: 2004-02-04
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the use of liquid porogen, the pore size of the flow holes in the above-mentioned macroporous medium is difficult to control (as reported in U.S. Patent No. 5,723, the pore size of the flow holes of the agarose gel medium reported in 601 is distributed at 0.5 to 1000 μm), and the preparation process It is more complicated; in addition, the residual organic porogen in the chromatography medium will also have a certain impact on the performance and use of the medium

Method used

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  • Macropore high-capacity agarose gel media preparing method
  • Macropore high-capacity agarose gel media preparing method
  • Macropore high-capacity agarose gel media preparing method

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Experimental program
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Embodiment 1

[0020]At 90°C, 4.5 g of the processed calcium carbonate particles with a particle size of 2.67 μm and a distribution of 0.86 to 4.58 m were uniformly dispersed in 50 mL of 60 g / L agarose solution by rapid stirring. Then quickly pour the suspension into 300mL organic phase containing 30g / L Span80, and control the stirring speed at 1200rpm at 90°C; after reacting for 30min, quickly cool down to 20°C, and continue to The solidification reaction was carried out for 40 minutes; water twice its volume was added to the above reaction system, and an agarose gel medium wrapped with calcium carbonate particles was obtained by natural sedimentation. The collected microspheres were washed repeatedly with acetone and distilled water successively to remove the residual organic phase. Take 50mL of washed microspheres and mix them with an equal volume of 1mol / L sodium hydroxide solution, then add 500mg of sodium borohydride to make the concentration of sodium borohydride in the suspension 5g / ...

Embodiment 2

[0022] At 85° C., 6 g of processed calcium carbonate particles with a particle size of 2.67 μm and a distribution of 0.86 to 4.58 μm were uniformly dispersed in 50 mL of 60 g / L agarose solution by rapid stirring. Then quickly pour the suspension into 300mL organic phase containing 30g / L Span80, and react for 15min at a wrapping temperature of 85°C and a stirring speed of 1300rpm; rapidly cool down to 15°C, and solidify while maintaining a constant stirring speed React for 30 minutes; add twice the volume of water to the above system, and obtain an agarose gel medium wrapped with calcium carbonate particles through natural sedimentation. The collected microspheres were washed repeatedly with acetone and distilled water until the organic phase was completely removed. Take 50mL of washed microspheres and mix them with an equal volume of 1mol / L sodium hydroxide solution, then add 400mg of sodium borohydride to make the concentration of sodium borohydride in the suspension 4g / L. T...

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Abstract

A process for preparing high-capacity microreticular agarose gel medium includes such steps as dispersing the granular calcium carbonate as hole-forming agent in the aqueous solution of agarose to obtain suspension, adding it to salad oil, adding emulsifier Span80, emulsifying while stirring, quick cooling for sphericizing, adding epoxy chloropropane, cross linking, reducing with sodium borohydride, and using diluted hydrochloric acid to remove granular calcium carbonate.

Description

technical field [0001] The invention relates to a preparation method of a high-capacity macroporous agarose gel medium, which belongs to the preparation technology of the agarose gel medium. Background technique [0002] In the downstream processing of biology, chromatographic separation has an irreplaceable position by other separation methods. The development of chromatography technology is premised on the development of separation media and the improvement of process theory, among which the development of new chromatography media has always been one of the research hotspots in the field of chromatography. An ideal chromatography medium should not only have a high adsorption capacity, but also meet the requirements of fast, high resolution and low non-specific adsorption. However, most of the current chromatographic media have pore sizes on the order of nanometers. The mass transfer coefficient of protein in the above media is two to three ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/08B01J20/24
Inventor 孙彦周鑫史清洪董晓燕白姝
Owner TIANJIN UNIV
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