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Tissue paraffin removing liquid

A technology for dewaxing liquid and tissue, which is applied in the field of pathology and can solve problems such as inability to dewax online

Inactive Publication Date: 2012-06-20
FUZHOU MAIXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because xylene will corrode the parts of the instrument, most automated stainers cannot be dewaxed online or choose other xylene substitutes for dewaxing

Method used

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  • Tissue paraffin removing liquid
  • Tissue paraffin removing liquid
  • Tissue paraffin removing liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0019] The research objects were formaldehyde-fixed-paraffin-embedded uterine leiomyoma, breast cancer, and tonsil tissues (pathology department of XX Hospital). The dewaxing steps are as follows: drop the dewaxing liquid on the slices to completely cover the tissue, treat at room temperature for 3-10 minutes, drain the dewaxing liquid on the surface of the slices, add new dewaxing liquid dropwise and repeat the above steps twice, tap water After washing, soak the slices in 100%, 100%, 95%, and 85% alcohol successively for 0.5-3 minutes, and finally rinse with water to complete.

[0020] At the same time, set up a xylene dewaxing control group. The dewaxing steps are as follows: put the tissue paraffin slices in the first vat of xylene, soak at room temperature for 3-10 minutes, take out the slices and put them in the second vat of xylene to repeat under the same conditions In the previous step, take out the slices and put them in the third vat of xylene to repeat the previous...

Embodiment 2

[0046] The research objects were formaldehyde-fixed-paraffin-embedded esophageal squamous cell carcinoma, lymphoma, breast cancer, gastric cancer, breast invasive ductal carcinoma, schwannoma, liver cancer, and tonsil tissues (pathology department of XX Hospital). The target antigens detected are: ki-67, CD43, C-erbB-2, p53, PR, S-100, CK8, ER, CD45RO, CD20.

[0047] The dewaxing steps are as follows: drop the dewaxing liquid on the slices to completely cover the tissue, treat at room temperature for 3-10 minutes, drain the dewaxing liquid on the surface of the slices, add new dewaxing liquid dropwise and repeat the above steps twice, tap water After washing, rinse with 75% alcohol for 3 times, each time for 0.5-3 minutes, and finally rinse with water to complete.

[0048] At the same time, set up a xylene dewaxing control group. The dewaxing steps are as follows: put the tissue paraffin slices in the first vat of xylene, soak at room temperature for 3-10 minutes, take out the...

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Abstract

The invention provides a tissue paraffin removing liquid, which includes vegetable oil. The paraffin removing liquid not only can be used for manually removing paraffin from tissue paraffin section in a routine laboratory, and but also can be used for on-line paraffin removing of the tissue paraffin section on an automatic dyeing instrument. The tissue paraffin removing liquid can completely replace xylene during the paraffin removing process, so as to achieve excellent dyeing effect and have the paraffin removing same effect as the xylene. The natural vegetable oil as the main component of the paraffin removing liquid is harmless to human and environment.

Description

technical field [0001] The invention belongs to the field of pathology, in particular, the invention relates to a dewaxing liquid for paraffin-embedded tissues or cells in the process of immunohistochemistry, immunocytochemistry and in situ hybridization. technical background [0002] At present, the production of most pathological tissue sections still adopts the traditional paraffin section production technology. In the process of pathological film preparation, xylene is generally used as a transparent and dewaxing agent in the three links of tissue transparency, section dewaxing before staining, and post-staining transparency. Although xylene has good dewaxing and clearing effects, it is a toxic substance with a strong pungent smell and can be absorbed into the body through the respiratory tract and skin. Xylene is irritating to the eyes and upper respiratory tract, and has an anesthetic effect on the central nervous system at high concentrations. Short-term inhalation ...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 熊玉林林齐心王小亚
Owner FUZHOU MAIXIN BIOTECH CO LTD
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