Making method of body fluid cell lump paraffin section

Abstract

This invention relates to body liquid cell olefin slice making process, which comprises pre-processing, decenter deposition and fixing dehydrate wax impregnation, burying, slicing and HE dying and sealing. The invention works together with daily work with clear background of HE and immune organization dying without dark background of agar burying and processing the molecule burying and improves the accuracy of the body liquid cell diagnose.

Description

technical field [0001] The invention is one of the detection methods for clinical medical detection of tumor cells and cell molecular markers. Background technique [0002] Under the action of carcinogenic factors, the nature of human cells will gradually change qualitatively and become cancer cells. Under the influence of long-term carcinogenic factors, the tumor cells formed by the cells will develop into malignant tumors when the number of tumor cells increases to a certain number. , in order to diagnose the disease, prescribe the right medicine, and strive for the best treatment, time and effect, one of the methods currently used by the medical department for patients is to find cancer cells in body fluids, and generally use a centrifuge to make a centrifugal sedimentation smear, or use agar It is simple and quick to use a smear centrifuge to embed cell block paraffin sections, but the equipment is more expensive, and cannot be used for immunohistochemical staining, cell...

AI Technical Summary

Problems solved by technology

[0002] Under the action of carcinogenic factors, the nature of human cells will gradually change qualitatively and become cancer cells. Under the influence of long-term carcinogenic factors, the tumor cells formed by the cells will develop into malignant tumors when the number of tumor cells increases to a certain number. , in order to diagnose the disease, prescribe the right medicine, and strive for the best treatment, time and effect, one of the methods currently used by the medical department for patients is to find cancer cells in body fluids, and generally use a centrifuge to make a centrifugal sedimentation smear, or use agar It is simple and quick to use a smear centrifuge to embed cell block paraffin sections, but the equipment is more expensive, and cannot be used for immunohistochemical staining, cell molecular marker detection, and more accurate detection of tumor patients. Difficult smears, it is difficult to classify tumor types, and sometimes it is difficult to distinguish between degenerated tumor cells and proliferative and degenerated mesothelial cells. There are many procedures for paraffin-embedded sectioning with agar cell blocks, and it is troublesome to make agar in advance. Therefore need to improve