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Preparation detection method of biofilm

A detection method, biofilm technology, applied in biochemical equipment and methods, microbial determination/inspection, sustainable biological treatment, etc., can solve problems such as no clear method

Inactive Publication Date: 2011-02-16
曾晓希
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The biofilm formed on the surface of biological ceramsite plays an important role in water treatment, but the current detection of biofilm can only be judged based on experience, and there is no clear method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Cleaning and sterilization of ceramsite: rinse ceramsite with water to remove dust and impurities, then place it in ultrasonic cleaning for 10 minutes, and sterilize at 121°C for 20 minutes.

[0018] (2) Cultivate microorganisms: add 200mL of liquid culture medium to a 500mL Erlenmeyer flask, inoculate the strain P.aeruginosaE1 cultivated to the logarithmic phase into the culture medium, and vibrate in a constant temperature shaker at 30°C with a rotation speed of 180rpm. After about 16 hours, the strain enters the mid-logarithmic growth phase, and the flask is taken out.

[0019] (3) Ceramsite film-hanging: add 100g of ceramsite after cleaning and sterilization into the conical flask treated in (2) on the ultra-clean bench, and then put the conical flask back into the shaker. , let the bacteria grow on the ceramsite.

[0020] (4) Biofilm detection: After the ceramsite was cultured in the culture medium for 6 hours, 6 medium-sized ceramsites were taken from the Erl...

Embodiment 2

[0022] (1) Cleaning and sterilization of ceramsite: rinse ceramsite with water to remove dust and impurities, then place it in ultrasonic cleaning for 10 minutes, and sterilize at 121°C for 20 minutes.

[0023] (2) Cultivate microorganisms: Add 150-200mL of liquid culture medium to a 500mL Erlenmeyer flask, inoculate the sulfate-reducing bacteria K4 cultivated to the logarithmic phase into the culture medium, and place them in a constant temperature shaker at 32°C and a speed of 180rpm Shake the culture, and the strain enters the middle logarithmic growth phase in about 18 hours, and the Erlenmeyer shaker flask is taken out.

[0024] (3) Ceramsite film-hanging: add 100g of ceramsite after cleaning and sterilization to the triangular difference treated in (2) on the ultra-clean bench, and then put the triangular flask back into the shaker, the culture condition is 32°C, the rotation speed is 100rmp , let the bacteria grow on the ceramsite.

[0025] (4) Biofilm detection: After...

Embodiment 3

[0027] (1) Cleaning and sterilization of ceramsite: wash ceramsite with water three times to remove dust and impurities, then place it in ultrasonic cleaning for 10 minutes, and sterilize at 121°C for 20 minutes.

[0028] (2) Cultivate microorganisms: add 150-200mL of liquid culture medium to a 500mL Erlenmeyer flask, inoculate the strain P.aeruginosa D2 cultivated to the logarithmic phase into the culture medium, and inoculate it in a constant temperature shaker at 30°C and a speed of 180rpm Shake the culture, and the strain enters the middle logarithmic growth phase in about 16 hours, and the Erlenmeyer shaker flask is taken out.

[0029] (3) Ceramsite film: add 100g of ceramsite after cleaning and sterilization into the triangular flask treated in (2) on the ultra-clean bench, then put the triangular flask back into the shaker, the culture condition is 30°C, the rotation speed is 100rmp , let the bacteria grow on the ceramsite.

[0030] (4) Biofilm detection: After the cer...

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Abstract

The invention relates to a preparation detection method of a biofilm. A stable microorganism biofilm can be formed on a carrier through the steps of ultrasonic carrier cleaning, microbiological culture, carrier biofilm formation, biofilm detection and the like. The specific surface area of the carrier can be increased by ultrasonically cleaning the carrier, so that the apposition growth of more microorganisms can be carried out on the biofilm, and the biomass of the microorganisms is increased. Biofilm formation is started from the metaphase of the logarithmic growth phase of the microorganisms, so that the time of biofilm formation can be shortened, and the efficiency of biofilm formation can be effectively increased. The growth condition of thalli on the biofilm can be accurately monitored in time by using the blood corpuscle counting mode. The preparation detection method of the biofilm is quick in screening and can be widely applied to the fields of environment water treatment, biomedicine materials and the like.

Description

technical field [0001] The invention relates to a preparation and detection method of a biofilm, in particular to a preparation of a ceramsite biofilm and a method for detecting the growth of biofilm microorganisms on the ceramsite. Background technique [0002] Biological pretreatment is a breakthrough and innovation to the traditional water treatment process, generally using biofilm method, mainly including biological contact oxidation, biological filter, biological turntable, biological fluidized bed, etc. In current industrial applications, bioceramsite is one of the main carriers of biofilm growth. Biological ceramsite is a granular material made of clay as the main raw material, mixed with a certain pore-forming agent and binder, and processed through a series of processes such as batching, agglomeration, high-temperature firing, and screening. It has the characteristics of high strength, friction resistance, stable physical and chemical properties, and no release of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/10C12Q1/06
CPCY02W10/10
Inventor 曾晓希汤建新刘学端蒋佩李文
Owner 曾晓希
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