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Method for improving mitotic phase of chrysanthemum root tip by artificial regulation

A technology of mitosis and chrysanthemum, which is applied in the research field of chrysanthemum cytology and molecular cytogenetics, can solve the problems of inability to obtain chromosome observation results, time-consuming, labor-intensive and other problems, and achieves outstanding practicability, strong operability, and simple and easy method. row effect

Active Publication Date: 2019-06-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

When using FISH technology for cytological research and analysis, there are high requirements for the chromosome division period and the integrity of the chromosome structure, and it is necessary to obtain a suitable chromosome division period and a chromosome with an intact chromosome structure. If the metaphase division phase is less and the structure is incomplete Chromosomal slides cannot obtain ideal chromosome observation results, which is time-consuming and labor-intensive

Method used

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  • Method for improving mitotic phase of chrysanthemum root tip by artificial regulation
  • Method for improving mitotic phase of chrysanthemum root tip by artificial regulation

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Embodiment Construction

[0022] 1. Determination of Dark Treatment Time

[0023] Place the tissue-cultured seedlings to be identified with 4-5 root systems with a length of about 2-3cm under dark conditions, treat them in the dark for 0h, 24h, and 48h respectively, and then take materials from their root tips according to the above method for mitotic chromosome preparation. Observe the distribution of mitotic phases in the preparation. The results showed that the mitotic mitotic phases were better and the metaphase mitotic phases were more in the dark treatment for 48 hours. Therefore, it is determined that the more appropriate dark treatment time is 48h.

[0024] 2. Determination of light processing time

[0025] Place the dark-treated tissue cultured seedlings under the sun on a sunny day, or at a distance of 30cm from a light source with a light intensity of 20,000Lux 15W, and treat them for 35min, 45min, and 55min respectively, and then use the above-mentioned method to obtain materials for mito...

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Abstract

The invention discloses a method for improving the mitotic phase of a chrysanthemum root tip by artificial regulation. With the artificial regulation method, chrysanthemum tissue-cultured seedlings are processed under the experiment-verified conditions of the proper dark processing and illumination (strong) time, so that the clean chromosome film is obtained and the metacinesis numbers of the mitosis metaphase of the cell can be increased effectively (shown in a figure I and a figure II). The utilized raw materials are available; the method is simple and is easy to implement; the cost is low;and the operability and practicability are high. On the basis of dark processing and illumination processing, the mitosis metaphase phase number is increased; and a clean root-tip mitotic metaphase chromosome image is obtained and the obtained image can be applied to identification of the chrysanthemum mitosis chromosome. Besides, the cytological study for karyotype analysis and FISH analysis andthe like can be realized. The method for acquiring the clear root-tip mitotic metaphase chromosome image of the chrysanthemum can be promoted to the cytological study of other chrysanthemum plants.

Description

technical field [0001] The invention belongs to the research field of chrysanthemum cytology and molecular cytogenetics, and relates to a method for improving the mitotic phase of chrysanthemum root tip through artificial regulation. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium) is a plant of the genus Chrysanthemum in the family Asteraceae. It is a famous flower and one of the four major cut flowers in the world. It has high ornamental and economic value. At present, the cytological research on Chrysanthemum plants mainly focuses on chromosome ploidy, chromosome banding, karyotype analysis, meiosis behavior and chromosome in situ hybridization, among which karyotype analysis and fluorescence in situ hybridization (Fluorescence in situ hybridization) situ hybridization (FISH) is an important means of plant classification and genetic research. The karyotype analysis of chrysanthemum is mainly carried out by the traditional pressing method for chromo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
Inventor 王海滨何俊陈发棣卢漫林思思房伟民陈素梅赵爽
Owner NANJING AGRICULTURAL UNIVERSITY
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