Method for identifying cotton D genome and D sub-genome complete chromosome

Abstract

The invention belongs to the field of molecular cytogenetics and particularly relates to a method for identifying D genome and D sub-genome complete chromosome. The method comprises the following step: by taking a sequence as shown in SEQ ID NO.1 as a probe to perform fluorescence in-situ hybridization on the DNA (deoxyribonucleic acid) of a cotton genome. The sequence is taken as the probe, and chromosomes in the mitosis metaphase of the upland cotton, the asiatic cotton and the Raymond's cotton are taken as target DNA to perform fluorescence in-situ hybridization, and the results indicate that obvious hybrid signals are shown on the D subgenome chromosome of the upland cotton and the D genome chromosome of the Raymond's cotton, the signals are distributed on all chromosomes, and no obvious signals are shown in the A subgenome chromosome of the upland cotton and the A genome chromosome of the asiatic cotton. The sequence adopted by the method disclosed by the invention can be used for quickly identifying the cotton D genome and D sub-genome complete chromosome by virtue of a FISH (fluorescence in situ hybridization) method.

Description

technical field [0001] The invention belongs to the field of molecular cytogenetics, in particular to a method for identifying a complete set of chromosomes of cotton D genome and D sub-genome. Background technique [0002] Cotton is divided into 52 cotton species, including A, B, C, D, E, F, G and K8 chromosome groups. Today, the main cultivated species of cotton in the world are tetraploid cotton species upland cotton (AD) and sea island cotton (AD), which account for about 95% and 5% of the cultivated area respectively. At present, the genome sequencing of Group A cotton species Cotton chinensis and Group D cotton species Raymond has been completed (Wang et al., 2013; Li et al., 2014). The comparative study of cotton A and D genomes will be of great help to the evolution research of tetraploid cotton and cotton breeding. Fluorescence in situ hybridization technology was born in the 1960s, and its appearance opened a new era of cell and molecular biology. Subsequently, ...

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Problems solved by technology

However, in actual experiments, different types of reagents, different concentrations and differences in treatment time often lead to different degrees of shortening of chromosomes. In addition, the size of cotton chromosomes is small, the difference between chromosomes is not large, and the centromere is not easy. Factors such as discrimination make it difficult to obtain ideal and reliable results by relying on chromosome morphological characteristics to identify chromosomes

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