32 results about "Polarized light microscopy" patented technology

Apparatus for generating polychromatic polarized light with the polarization ellipse orientation determined by the wavelength. The proposed polychromatic polarization state generator can be used in various configurations of polarized light microscope (called “polychromatic polscope”) for imaging birefringent samples. The polychromatic polscope produces a spectral-modulated visual scene, in which birefringent structures are evident because their appearance is different from the background. New polarized light microscope can subtract the background and produce video-enhanced color image of birefringent structures. The obtained picture can be also mathematically processed in order to obtain a map of quantitative distribution of specimen retardation and orientation of the principal axes.

Disclosed is a method for measuring a fiber content of polyethyleneterephthalate (PET) and polytrimethyleneterephthalate (PTT) fibers, particularly wherein the method includes: a qualitative analysis step of identifying a presence of polyethyleneterephthalate (PET) and polytrimethyleneterephthalate (PTT) fibers in a mixed-spun fiber product; and a quantitative analysis step of measuring the fiber content by a microscopic count through difference in color between PET and PTT fibers by birefringence in a cross polarization state of a polarized light microscope (PLM). Through the present invention, it is possible to simply and accurately calculate the fiber content of PET and PTT fibers in a mixed-spun fiber material in which various kinds of fibers are mixed.

The invention provides a staining method for observing collagen fibers with a polarized light microscope. The method is to stain with saturated picric acid-Sirius red staining solution at 70°C-90°C, then soak the tissue sections with a weak acid solution with a concentration of 1-10% w / v, and finally soak the tissue sections as usual. Washing, drying, and fixing; the heated picric acid has a stronger penetration effect on the tissue, making the background of the lining more vivid, and the heated Sirius red dye solution is also more firmly adsorbed on the collagen fibers, and the coloring is clear; the weak acid solution can be washed off The crystallization of picric acid precipitated in the tissue section is not easy to wash off the background of the yellow lining, so as to ensure that the collagen fibers are well colored, the background of the yellow lining is obvious, and the collagen fibers are clear; the dyeing method of the present invention has good repeatability, and the collagen fibers are colored Obviously, the fiber structure is clear. This method is conducive to the positioning and quantitative analysis of different types of collagen fibers, and is convenient for judging the degree of tissue fibrosis.