Method for cell imaging by adopting polarized light microscope to observe nano particles
A polarizing microscope and nanoparticle technology, applied in the field of medical detection, to achieve the effect of strong background reduction
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[0026] 1. Reagents
[0027] Sodium citrate, chloroauric acid (99.99%, HAuCl 4 3H2O), cetyltrimethylammonium bromide (99%, CTAB) were all purchased from Shanghai Sinopharm Company; sodium borohydride (98%, NaBH 4 ), ascorbic acid (99.7%, AA), silver nitrate (99.8%, AgNO 3 ), mercaptoundecyltrimethylammonium bromide (99%, MUTAB) were purchased from Sigma-Aldrich Company. The above reagents were prepared with ultrapure water when used.
[0028] Aqua regia (volume ratio HCl:HNO 3 =3:1) Perform soaking and cleaning.
[0029] 2. Synthesis of antibody-modified gold nanorods
[0030] 1) Synthesis of gold nanoparticle seeds: In a serum bottle, inject 48 μL of 0.1M ice-cold sodium borohydride containing 0.1M CTAB and 0.00025M HAuCl 4 In the 4mL solution, the solution needs to stand in the solution at 28°C for more than 2 hours before use.
[0031] 2) In 20mL 0.1M CTAB solution, add 426μL 24.28mM HAuCl in sequence 4 ,0.5ml 4mM AgNO 3 and 105μL 0.1M AA; when the solution turns f...
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