48 results about "Cytogenetics" patented technology

Genes and gene expression profiles useful for predicting outcome, risk classification, cytogenetics and / or etiology in pediatric acute lymphoblastic leukemia (ALL). OPAL1 is a novel gene associated with outcome and, along with other newly identified genes, represent a novel therapeutic targets.

The invention relates to the field of cytogenetics and the application of genetic diagnostic techniques in pathology and hematology. Specifically, the invention relates to nucleic acid probes that can be used in hybridization techniques for the detection of chromosomal aberrations and other gene rearrangements such as immunoglobulin and T-cell receptor gene rearrangements. The probes provided by the invention are a distinct and balanced set of probes of comparable size, each preferably being from 1 to 100 kb, or smaller, and flanking a potential breakpoint in a chromosome.

The invention discloses a biological sample processing system which comprises a system base plate, a glass slide placing platform, a sample cell, a biological sample dropping device, a sample processing cavity body, a control system and a human-computer interface, wherein the biological sample dropping device comprises a space motion mechanism, a pipetting device and a glass slide taking and putting device, and the space motion mechanism is controlled by the control system, so that a preset moving track is output by a moving part of the space motion mechanism, a glass slide is automatically arranged at the beginning of an experiment, a biological sample is automatically sucked and transferred onto the surface of the glass slide by the pipetting device, the glass slide is automatically removed after the completion of the experiment, and the temperature of the biological sample cell can be adjusted. According to the biological sample processing system disclosed by the invention, the automatic dropping operation of chromosomes can be performed in a high-throughput mode; the biological sample processing system has the advantages of small size, low production cost, convenience in installation and maintenance and low energy consumption and is especially suitable for analyzing cytogenetics; the dropping process automation of the chromosomes is realized, the analysis efficiency is improved, and the biological sample processing quality is guaranteed.

Methods and compositions utilizing an miRNA signature for the diagnosis, prognosis and / or treatment of leukemia associated diseases, particularly acute myeloid leukemia, are disclosed.

Methods and compositions for the diagnosis, prognosis and / or treatment of leukemia associated diseases are disclosed.

The invention provides a PDX model establishing method for granulocyte tumors, and relates to the technical field of animal models. According to the PDX model establishing method, PDX models are established for AML of different FAB types, pathogenesis is clear, stable passage is achieved, cytogenetics changes are consistent with a patient, the models are ideal platforms for researching the pathogenesis of the AML and screening targeted drugs, the requirements of various hematology experiment bases and pharmaceutical companies are met, and huge economic benefits and social benefits can be generated. The three PDX models of M2a, M4 and M5 for the AML are established by utilizing the method disclosed by the invention, pathogenesis is clear, stable passage is achieved, homogeneity is good, themodels can be used for large-scale drug screening experiments, and good application prospects are achieved.

The present invention relates to non-transgenic tetraploid wheat plants having soft textured endosperm, methods for constructing said non-transgenic plants using cytogenetics and classical breeding techniques, endosperm therefrom and uses thereof.

The invention discloses a small-molecule fluorescent probe and an application thereof in chromosome analysis and cytogenetics, and the small-molecule fluorescent probe can be used as a universal toolfor chromosome image segmentation and is used for identifying and distinguishing overlapped, aggregated or contacted chromosomes. The small-molecule fluorescent probe is used as a reference marker forpositioning centromere positions, is used for classifying homologous chromosomes, distinguishing different forms of chromosomes and estimating chromosome arm lengths, is used in cooperation with karyotype analysis for chromosome deletion and polyploid, is used in cooperation with fluorescence in situ hybridization (FISH), and is used for positioning designated genes and judging chromosome abnormalities. The invention provides a rapid and reliable method for auxiliary chromosome analysis, and has great potential in conventional clinical practice.

The invention relates to the field of cytogenetics and the application of genetic diagnostic techniques in pathology and hematology. Specifically, the invention relates to nucleic acid probes that can be used in hybridization techniques for the detection of chromosomal aberrations and other gene rearrangements such as immunoglobulin and T-cell receptor gene rearrangements. The probes provided by the invention are a distinct and balanced set of probes of comparable size, each preferably being from 1 to 100 kb, or smaller, and flanking a potential breakpoint in a chromosome.

The invention belongs to the field of cytogenetics and cell biology, and relates to a method for preparing a chromosome sample. Aiming at the problem that it is often difficult for operators to obtain enough metaphase chromosome specimens in the production of chromosome specimens, the present invention discloses a method for preparing wheat chromosome specimens, which includes seed soaking, germination, low temperature treatment, rewarming growth, pre- Processing, fixation and other steps. The method of the invention is simple to operate, and even a beginner can obtain enough metaphase cleavage phase by using the method.

The invention provides a culture medium for flounder paralichthys lymphocyte proliferation. The culture medium is formed by adding concanavalin A, lipopolysaccharide, phytohemagglutinin, America pokeweed lectins, flounder paralichthys serum, fetal calf serum, mixed deoxygenation mononucleotide, L-glutamine and sodium bicarbonate into a basic culture medium. The culture medium contains the flounder paralichthys serum and various types of mitogen for promoting proliferation of lymphocyte, through effective matching of all ingredients, efficient and rapid proliferation of the lymphocyte can be achieved, the activity index of the lymphocyte is high, the average proliferation activity is as high as 85.33%, and meanwhile the culture medium has good stability and can completely meet the requirement of culturing lymphocyte of the fish molecular immunology and the cytogenetics.

The invention provides a method for identifying the centromere chromosome in the middle part of the scallop. By combining the genome sequence information of the scallop, the SNP linkage map information and the fosmid monoclonal decoding information, three chromosomes distributed on the SNP linkage map of the scallop are selected. Screen the fosmid library with each marker, use the clones containing SNP sites to prepare fluorescently labeled probes, and then use these probes for fluorescence in situ hybridization and co-hybridization, according to the three specific clones containing SNP markers The distribution of the fluorescent signal on the chromosomes of Scallop scallops realized the specific recognition of three pairs of mesocentromere chromosomes in scallops. The three markers provided by the present invention realize the identification of three pairs of middle centromere chromosomes, and are of great significance to the discrimination and identification of the chromosomes of the scalloped scallop, the construction of the cytogenetics map, and the evolution analysis of chromosomes from different species .

The invention discloses a new application of cholesterol transporter gene and / or its encoded protein in the preparation of acute myeloid leukemia therapeutic drugs. The invention screens out the molecular markers related to the diagnosis and prognosis of acute myeloid leukemia (AML), and screens out the cholesterol transporter gene (GRAMD1C) which is closely related to the prognosis of AML patients and is differentially expressed in AML; GRAMD1C is correlated with FAB typing and cytogenetic risk stratification in AML patients. The higher the cytogenetic risk, the higher the expression of GRAMD1C; the high expression of GRAMD1C indicates poor prognosis in AML patients; GRAMD1C may participate in Wnt signaling pathway, regulate Transcription factor activity, etc. are involved in the disease progression of AML. GRAMD1C can be used in diagnostic and prognostic research formulations for AML.

Staining nucleotides within chromosomes is an important technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. Such techniques are useful, for example, in identifying genetic diseases through the photographic representation of the entire chromosome complement. Prior to the steps of selective dissolving and staining of nucleotides within the chromosomes an aging step is performed to increase the selective solubility between different nucleotides. Within the prior art this aging step is a time consuming process taking several days. Accordingly, it would be beneficial to provide a staining pretreatment method which can be performed in a short period of time.