42 results about "Cell mechanics" patented technology

A kind of cell pulls to stretch to add to carry device, involve a medical science experiment instrument in of the cell mechanics add to carry device. The invention mainly includes a machine, motive system and spreads to move system and pulls to stretch sport part. Motive system mainly from controller, actuator, tread to constitute into the electrical engineering; Spread to move the system main grenadine and pull pole composing; Pull to stretch sport part mainly from glide a support, fix support, direction pole, pioneer pole piece and behind lead a pole piece composing. Because the invention has and pull and stretch process to the cell steady, the cell is subjected to dint even, the ability accurate control pulls and stretches range and frequency, pulling and stretching distance can the convenience regulates within the scope of 0~60 mm, can make under same condition of the experiment have good consistency etc. characteristics, so the invention can used to a medical science experiment the cell mechanics of the instrument and add and carry device extensively, is the ideal which carries on mechanics incitement and responds to a research to the living creature organization and the cellin the biomechanics and add and carry device.

A sinusoidal tension cell loading device relates to a cell mechanical loading device in medical experiment instruments. The invention mainly includes a frame, a gear reduction speed regulating motor, a digital display speed governor, a crank slider mechanism and a stretching movement mechanism. The slider crank mechanism is mainly composed of an adjusting screw rod, a crank arm slider, a crank and a pull rod; the stretching motion mechanism is mainly composed of a sliding bracket, a fixed bracket, a guide rod, a front guide rod block and a rear guide rod block. Since the present invention has the characteristics of simple structure, easy operation, convenient maintenance, low cost, stable operation, high cost performance, uniform stress on cells, adjustable stretching amplitude and frequency, etc., the stretching distance can be easily adjusted within the range of 0-25mm, It can meet the mechanical stimulus response research of tensile loading of various adherent growth cells, so the present invention can be widely used as a cell mechanical loading device in medical experimental instruments, and is especially suitable for wide application in research institutions lacking funds and professional operators .

One embodiment of the present invention provides a system that measures single cell mechanics using a scanning probe microscope. During operation, the system positions a modified probe of the scanning probe microscope above a cell which is located on a surface, wherein the modified probe is configured with a geometry for compressing the cell. The system then comprises the cell against the surface using the modified probe, thereby causing the cell to deform. Next, the system extracts mechanical properties of the cell from cell deformation behavior and cell response to the compression force.

The invention provides a dynamic non-contact cell mechanical stimulation loading device. A plurality of pipelines are formed in a wall of a culture cavity to be communicated with a circulation water bath; the culture cavity is fixed in a box body, and the box body is filled with heat insulation materials; the output of an air compressor and a CO2 pot is input into the culture cavity after being subjected to matching control through a gas flowmeter; a plurality of humidifying vessels are placed in the culture cavity, and a humidity measuring instrument is used for detecting the humidity inside the culture cavity; two same rotary plates rotate around a perpendicular rotating shaft under the drive of a motor, each rotary plate is provided with a permanent magnet, an object stage is installed between the two rotary plates, an experiment object is placed on the object stage, and the object stage is fixed instead rotating. Various dynamic non-contact cell mechanical stimulation loading ways can be achieved, space non-contact extrusion type, periodic gradient type and multi-dimensional uniform type mechanical stimulation effects are applied to cells, and an in-vitro cell culture environment is built for the cells.

The present invention proposes a genetic engineering algorithm based on a matrix model of cell mechanics, which specifically includes the following steps: 1) setting the theory of cell mechanics, 2) selecting a theoretical system derived from classical mechanics to establish complex calculations for cells; 3) combining the Hamiltonian principle, Its characteristic is described by Lagrangian function, 4) according to 3) derivation obtains cell mechanics equation; 5) according to 4), cell mechanics equation can be written as equivalent matrix form: H|X>=|X '>; the present invention provides a theoretical basis for genetic engineering design, and conducts computer prediction and evaluation of genetic engineering products in the design stage; the present invention can mathematicize biological theories, thereby applying the results of human research to robot design In the rapid development of artificial intelligence, since the human genome is the best operating system, the present invention can apply the design method of the human genome operating system to the computer operating system design.

Embodiments of the present disclosure provide a photonic crystal microscope and a method for measuring cell mechanics. The photonic crystal microscope includes a photonic crystal substrate, a stage, a detection light source and an imaging component. The photonic crystal substrate is above the stage, and the detection light source and imaging components are sequentially located on the side of the stage away from the photonic crystal substrate. The photonic crystal substrate is used for Cultivate the cells to be tested, and when the cells to be tested grow on the photonic crystal substrate, the photonic crystal substrate can be deformed; wherein, the detection light source is used to send detection light to the photonic crystal substrate; the photonic crystal substrate reflects the detection light to the imaging component The imaging component receives the reflected light from the photonic crystal substrate for imaging, so as to obtain the force information between the cells to be measured and the photonic crystal substrate by using the imaging graphics. On the premise of ensuring the accuracy of subcellular measurement, the throughput is greatly improved, the complexity of the algorithm is simplified, the time resolution is improved, and the cost of the device and the complexity of the experiment are reduced.

The invention provides a mechanics analysis device for piezomodulated and sensed single cell, comprising a piezoelectric chip, a detecting tank, and a controlling and detecting system. The mechanics analysis device is characterized in that the piezoelectric chip is encapsulated in the detecting tank; the mechanic dynamic analysis on the piezomodulated and sensed single cell is carried out by controlling an detecting system; the piezoelectric chip consists of a piezoelectric sheet and at least one group of electrodes arranged on the surface of the piezoelectric sheet; one group of electrode pairs and the piezoelectric sheet form a piezoelectric resonator; the electrode pair comprises at least one micro-electrode with the area matched with that of the single cell and surface decorated chemically; the single cell is naturally fixed on the chemically decorated micro-electrode in a bonding way; the microelectrode can generate vibration by the controlling and detecting system and can interact with the bonding cell, thus synchronously realizing the dynamic modulation and real-time induction of the single cell mechanics. The device and the method aim at solving the problem of difficulty on modulation and monitoring of the cell mechanics in the single cell mechanics technology and have the characteristics of no existence of non-mechanic interference, high simpleness and flexibility, high flux and the like; the bio-mechanic characteristics of the cell can be discovered on cell layer, time and space; and the mechanics analysis device can be suitable for application in the wide fieldssuch as biomechanics, tissue engineering, drug screening, clinical medicine, and the like.

The invention discloses and belongs to the technical field of cell analysis and measurement, in particular to a single-cell mechanical intrinsic parameter measurement system and method based on electrical impedance signals. The single-cell mechanical intrinsic parameter measurement system based on electrical impedance signals is composed of a microfluidic system, a multi-frequency impedance measurement system and a real-time processing algorithm system in series: the measurement method of the single-cell mechanical intrinsic parameter measurement system includes impedance measurement system measurement , Collecting the impedance value of a single cell is to use the spatial coupling between the flow channel structure and the electrode position, as well as the high temporal resolution characteristics of the electrical impedance measurement electrical signal, to obtain the spatiotemporal morphological information of a single cell, and to achieve a single cell by fitting the single cell mechanical model. High-throughput real-time intrinsic mechanical parameter measurement of cells. The invention can solve the dynamic space position and deformation of cells without the need of a traditional high-speed camera, and realize the high-throughput real-time measurement of the mechanical intrinsic parameters of a single cell.

The invention provides a mechanical dynamic regulation and control chip for a single cell total environment. The chip provided by the invention comprises a micro-fluid control layer, a piezoelectric vibration layer and at least one single cell pool and is characterized in that the micro-fluid control layer and the piezoelectric vibration layer are integrated on the chip; the mechanical dynamic regulation and control of a fluid medium with single cells is carried out by regulating the direction and flow velocity of the micro-fluid of the chip; and simultaneously, the mechanical dynamic regulation and control of an extracellular matrix with single cells is carried out by regulating the amplitude and frequency of the piezoelectric vibration of the chip, thereby simultaneously realizing the mechanical dynamic regulation and control of all environments with single cells on the chip. The chip provided by the invention aims to solve the problem of difficulty in dynamic regulation and controlof an environment in a single cell mechanics technique, does not have mechanical dynamic regulation dead zones, has the characteristics of simplicity, convenience and flexibility in operation and high flux, can be used for discovering the biomechanics characteristics of cells in time and space and is suitable for being applied in wide fields such as biomechanics, tissue engineering, drug screening, clinical medicine and the like.

The invention relates to a microfluidic chip realizing high-throughout single-cell micro-tube sucking based on a wheatstone bridge, belonging to the field of microfluidic chip systems, and aiming at realizing high-throughout single-cell micro-tube sucking and measuring the single-cell mechanical property. The device simulates the wheatstone bridge structure, each unit of the device comprises fiveparts including a bridge channel and parallel channels in four parts which are divided by the bridge channel, by controlling the sizes of the five parts, the flow resistance among the five parts has certain proportional relation, then the control over the flowing direction and the like in the bridge channel is realized, further, the single-cell micro-tube sucking is realized, by controlling the input flow, the differential pressure between two ends of the cell during micro-tube sucking can be controlled, and thus the research for the single-cell mechanical property is facilitated. The microfluidic chip can be used for researching the single-cell micro-tube sucking and the single-cell mechanical property.