42 results about "Cell mechanics" patented technology

A kind of sine pulls to stretch cell to add to carry device, involve a medical science experiment instrument in of the cell mechanics add to carry device. The invention mainly includes a machine, wheel gear deceleration to adjust soon electrical engineering, number to show to adjust soon a machine, crank a slippery piece organization and pulls to stretch sport organization. The crank slips a piece of organization mainly from adjust a pole, the song arm slippery piece, crank and pull pole composing; Pull to stretch sport organization mainly from glide a support, fix support, direction pole, pioneer pole piece and behind lead a pole piece composing. Because the invention has structure simple, operate simple, maintenance convenience, the cost is cheap, circulating steady, sex price ratio high, the cell is subjected to dint even, can regulate to pull to stretch characteristics, such as range and frequency...etc., pull to stretch distance can the convenience regulates within the scope of 0~25 mm, can satisfy various cell which stick a wall growth to carry on the mechanics incitement which pulls to stretch to add to carry to respond to a research, so the invention can used to the cell mechanics within instrument of the medical science experiment to add to carry device extensively, being applicable to funds and profession to operate the think factory that the personnel lacks specially extensively applied.

A kind of cell pulls to stretch to add to carry device, involve a medical science experiment instrument in of the cell mechanics add to carry device. The invention mainly includes a machine, motive system and spreads to move system and pulls to stretch sport part. Motive system mainly from controller, actuator, tread to constitute into the electrical engineering; Spread to move the system main grenadine and pull pole composing; Pull to stretch sport part mainly from glide a support, fix support, direction pole, pioneer pole piece and behind lead a pole piece composing. Because the invention has and pull and stretch process to the cell steady, the cell is subjected to dint even, the ability accurate control pulls and stretches range and frequency, pulling and stretching distance can the convenience regulates within the scope of 0~60 mm, can make under same condition of the experiment have good consistency etc. characteristics, so the invention can used to a medical science experiment the cell mechanics of the instrument and add and carry device extensively, is the ideal which carries on mechanics incitement and responds to a research to the living creature organization and the cell in the biomechanics and add and carry device.

The invention belongs to the field of bone tissue engineering and cell mechanics, relating to a dynamic load and recirculating perfusion bioreactor, which comprises a main body, a sliding sleeve module and a three-dimensional culture cabin module, wherein the main body comprises a base, a side support plate and an upper support plate; the side support plate is provided with a concave sliding groove; the sliding sleeve module comprises a sliding sleeve, a piezoelectric ceramic, an up / down adjusting screw and a fastening screw; the sliding sleeve can upward and downward slide in the concave sliding groove stably; the piezoelectric ceramic passes through the sliding sleeve; the three-dimensional culture cabin module comprises a culture cabin main body of which the upper surface is provided with a through hole; the piezoelectric ceramic penetrates into the three-dimensional culture cabin through the through hole, the moving end of the piezoelectric ceramic is connected with a loading rod; and the culture cabin main body is also provided with a liquid inlet and a liquid outlet which are externally connected with a peristaltic pump. The invention has the characteristics of simple operation, high practicability, good aseptic sealing performance, high loading stress output accuracy, and timely and accurate data tracking records.

The invention discloses a micro-fluidic chip-level extracorporeal circulation system for vascular endothelial cell mechanics biological research, and belongs to the technical field of cell mechanics biological experiment devices. The system comprises three parts: 1) the micro-fluidic chip is composed of a sandwich structure cell culture cavity and a multi-element fluid mechanics loop for simulating hemodynamic characteristics; 2, the fluid loading device is combined with a feedback control system, and hemodynamic signals such as blood pressure, wall shear stress and circumferential stretchingstrain borne by different target artery endothelium can be generated in the cell culture cavity; 3) the signal acquisition and processing system can be used for observing cell mechanics biological response in real time and feeding back detection data to the control system so as to further adjust the fluid loading device. The system accurately simulates a real target artery vascular endothelial extracellular hemodynamic microenvironment, and provides a miniaturized, objective, standardized and quantified experimental platform for researching a quantitative relationship between hemodynamic signals and a vascular endothelial cell mechanics biological mechanism.

The invention provides a mechanical dynamic regulation and control chip for a single cell total environment. The chip provided by the invention comprises a micro-fluid control layer, a piezoelectric vibration layer and at least one single cell pool and is characterized in that the micro-fluid control layer and the piezoelectric vibration layer are integrated on the chip; the mechanical dynamic regulation and control of a fluid medium with single cells is carried out by regulating the direction and flow velocity of the micro-fluid of the chip; and simultaneously, the mechanical dynamic regulation and control of an extracellular matrix with single cells is carried out by regulating the amplitude and frequency of the piezoelectric vibration of the chip, thereby simultaneously realizing the mechanical dynamic regulation and control of all environments with single cells on the chip. The chip provided by the invention aims to solve the problem of difficulty in dynamic regulation and controlof an environment in a single cell mechanics technique, does not have mechanical dynamic regulation dead zones, has the characteristics of simplicity, convenience and flexibility in operation and high flux, can be used for discovering the biomechanics characteristics of cells in time and space and is suitable for being applied in wide fields such as biomechanics, tissue engineering, drug screening, clinical medicine and the like.

The invention provides a mechanics analysis device for piezomodulated and sensed single cell, comprising a piezoelectric chip, a detecting tank, and a controlling and detecting system. The mechanics analysis device is characterized in that the piezoelectric chip is encapsulated in the detecting tank; the mechanic dynamic analysis on the piezomodulated and sensed single cell is carried out by controlling an detecting system; the piezoelectric chip consists of a piezoelectric sheet and at least one group of electrodes arranged on the surface of the piezoelectric sheet; one group of electrode pairs and the piezoelectric sheet form a piezoelectric resonator; the electrode pair comprises at least one micro-electrode with the area matched with that of the single cell and surface decorated chemically; the single cell is naturally fixed on the chemically decorated micro-electrode in a bonding way; the microelectrode can generate vibration by the controlling and detecting system and can interact with the bonding cell, thus synchronously realizing the dynamic modulation and real-time induction of the single cell mechanics. The device and the method aim at solving the problem of difficulty on modulation and monitoring of the cell mechanics in the single cell mechanics technology and have the characteristics of no existence of non-mechanic interference, high simpleness and flexibility, high flux and the like; the bio-mechanic characteristics of the cell can be discovered on cell layer, time and space; and the mechanics analysis device can be suitable for application in the wide fieldssuch as biomechanics, tissue engineering, drug screening, clinical medicine, and the like.

One embodiment of the present invention provides a system that measures single cell mechanics using a scanning probe microscope. During operation, the system positions a modified probe of the scanning probe microscope above a cell which is located on a surface, wherein the modified probe is configured with a geometry for compressing the cell. The system then comprises the cell against the surface using the modified probe, thereby causing the cell to deform. Next, the system extracts mechanical properties of the cell from cell deformation behavior and cell response to the compression force.

The embodiment of the invention provides a photonic crystal microscope and a cell mechanics measurement method. The photonic crystal microscope comprises a photonic crystal substrate, an objective table, a detection light source and an imaging assembly, wherein the photonic crystal substrate is arranged above the objective table, the detection light source and the imaging assembly are sequentiallylocated on the side, deviating from the photonic crystal substrate, of the objective table, the photonic crystal substrate is used for culturing to-be-detected cells, and when the to-be-detected cells grow on the photonic crystal substrate, the photonic crystal substrate can deform; specifically, the detection light source is used for emitting detection light to the photonic crystal substrate; the photonic crystal substrate reflects the detection light to the imaging assembly; and the imaging assembly is used for receiving the reflected light from the photonic crystal substrate for imaging soas to obtain acting force information between the to-be-detected cells and the photonic crystal substrate by utilizing an imaging graph. On the premise of ensuring the subcellular measurement precision, the flux is greatly improved, the complexity of the algorithm is simplified, the time resolution is improved, and the device cost and the experiment complexity are reduced.

The invention provides a multi-cell mechanical loading device. The loading device comprises a piston of which the upper end is provided with a brace and a loading box of which one side is provided with an opening, wherein the specification of the piston is consistent with the internal specification of the loading box; the bottom of the loading box is provided with a sensor; and a signal acquisition instrument is arranged outside the loading box. The invention also provides a multi-cell mechanical simulation experiment platform. The experiment platform comprises the multi-cell mechanical loading device as mentioned above, a computer system and a universal material testing machine with a clamp, wherein the multi-cell mechanical loading device is fixed on the clamp; and the computer system is connected with the universal material testing machine. The invention has the following advantages: through the up-and-down movement of the piston, air is compressed to load pressure to cells, the stress uniformity of the cells can be ensured, and the accuracy of the determination result can be improved; the movement of the piston is controlled by the universal material testing machine, the pressure loading process can be exactly simulated, rapid response is achieved, and a complicated pressure change process can be simulated; and the multi-cell mechanical simulation experiment platform has the advantages of simple structure and convenience in operation.

The invention discloses a cell mechanical property tester based on STM32. The cell mechanical property tester based on STM32 comprises a quartz crystal receiving device, a frequency generation part, afrequency collecting part and a microcomputer processing part, wherein the quartz crystal receiving device comprises a quartz crystal and a film electrode; the frequency generation part consists of an oscillation circuit, a filtering circuit and an AGC (automatic gain control) circuit which are sequentially connected; the quartz crystal receiving device is connected with the oscillation circuit through an electric conducting wire; the frequency collecting part comprises a frequency / voltage conversion circuit; the microcomputer processing part comprises an STM32 chip and an external interfacethereof; the frequency / voltage conversion circuit is connected with the STM32 chip through an electric conducting wire. The cell mechanical property tester based on STM32 has the advantages that by adopting the non-invasion and non-marking testing type, the real-time, continuous and long-time testing is realized; the cell mechanical property tester is suitable for the cell mass, and the study andquantitative testing on the cell function can be performed.

The present invention discloses small molecule compounds as activators of myosin II by promoting its assembly and recruitment to contractile structures in the cell and methods of using such compounds. These compounds are useful to modulate cell and tissue mechanics. This class of molecules, which affect cell mechanics either by activating the contractile system of the cell or modulating cytokinesis, will be used for therapeutic and tissue engineering applications.

The invention discloses a microfluidic detection system and a microfluidic detection method for real-time flow velocity of a fluid. The system comprises a programmable control pump 1 connected with aninjector, a fluorescence microscope 5 and a computer 7, and further comprises a uniform and flat double-inlet micro-fluidic chip 4, wherein the uniform and flat double-inlet micro-fluidic chip 4 comprises a first liquid inlet pipe 9, a second liquid inlet pipe 10 and a convergence channel 11 and is a Y-shaped pipeline, the cross section of the pipeline is rectangular, scales 12 are arranged on the convergence channel, the uniform and flat double-inlet micro-fluidic chip 4 is movably arranged on the objective table of the fluorescence microscope 5, and the computer 7 is respectively and electrically connected with the programmable control pump 1 and the fluorescence microscope 5. According to the microfluidic detection system and the microfluidic detection method of the invention, the flowvelocity of a to-be-detected fluid can be rapidly detected, ie., the real-time flow velocity of the to-be-detected fluid, so that the basis is provided for quantitatively researching the movement rule of particles or fluids in a micro-channel and the cell mechanics behaviors.

The present invention relates to a measuring apparatus, and discloses a measuring apparatus and a measuring method of cell mechanics. The measuring apparatus comprises: a sample tray (1) for placing a cell sample, a measuring unit (2) for contacting the cell sample in the sample tray (1) to acquire the mechanical parameters of the cell sample, and a control unit (3) connected with the measuring unit (2) and used for controlling the measuring unit (2) to vertically move according to the mechanical parameters so as to change the contacting degree of the measuring unit (2) and the sample cell and make the measuring unit (2) acquire the mechanical parameters of the cell sample within the contacting degree change period. With the measuring apparatus, the mechanical parameters of the cell can be accurately acquired.

The invention belongs to the technical field of cell analysis and measurement, and particularly relates to a measurement system and method for mechanical intrinsic parameters of a single cell based on an electrical impedance signal. The measurement system for the mechanical intrinsic parameters of the single cell based on the electrical impedance signal is composed of a microfluidic system, a multi-frequency impedance measuring system and a real-time processing algorithm system which are connected in series. The measurement method of the measurement system for the mechanical intrinsic parameters of the single cell comprises the following steps: carrying out measuring by using the impedance measuring system; acquiring the impedance value of the single cell by utilizing the spatial coupling of a flow channel structure and an electrode position and by using the high time resolution characteristic of an electrical impedance measurement electric signal, thereby obtaining the spatial-temporal form information of the single cell; and conducting high-flux real-time intrinsic mechanical parameter measurement on the single cell by fitting a single cell mechanical model. According to the method, the dynamic spatial position and deformation of the cell can be solved without a traditional high-speed camera, and high-flux real-time measurement of the mechanical intrinsic parameters of the single cell is realized.

The invention discloses a cell culture device for simulating a cell mechanical microenvironment. The cell culture device comprises a gas driving device, a culture tank and a bulk acoustic wave generator, wherein a soft pneumatic cavity is arranged in the culture tank, a culture solution is inversely arranged on the surface of the soft pneumatic cavity, the soft pneumatic cavity is inflated and deflated through the gas driving device, the top of the soft pneumatic cavity is deformed, the deformation force of the soft pneumatic cavity is used as a loading method for macroscopic level mechanical stimulation of tissues, meanwhile, bulk acoustic waves are applied to cells in the culture solution through the bulk acoustic wave generator, the bulk acoustic waves are used as a loading method for microcosmic level mechanical stimulation of the cells, the bulk acoustic waves can act on all types of cells, accurate control over cells is achieved, meanwhile, sound wave control cannot cause damage to the cells, the sound flow control technology and the soft driving technology are combined, the cell culture device can give consideration to macroscopic and micromechanical stimulation on the cells, and simulation of the cell mechanical microenvironment in the in-vitro micro tissue culture process is achieved.

The invention relates to a cell culture room, an adjustable cell mechanical stimulation culture device and a manufacturing method thereof, the cell culture room comprises an elastic box made of an elastic material, and at least one culture compartment is arranged in the elastic box. PDMS is poured into the mold, the cell culture room is obtained after molding, and the PDMS comprises 1 part of a cross-linking agent and 10-50 parts of a substrate solution. The adjustable cell mechanical stimulation culture device comprises at least one mechanical loading unit and the cell culture chamber, each mechanical loading unit comprises a longitudinal fixing sheet, a left loading plate fixing sheet, a right loading plate fixing sheet and a longitudinal loading plate fixing sheet; and the left loading plate fixing sheet, the right loading plate fixing sheet and the longitudinal loading plate fixing sheet are connected with driving parts for driving the fixing sheets to move linearly. According to the invention, multi-time-sequence mechanical loading observation can be realized in the same batch, so that the controllability and authenticity of an in-vitro test are improved to a great extent; and the purposes of controllability of a single mechanical loading module, multi-parameter adjustability, flexible assembly of internal and external stress and the like can be achieved.

The invention provides a mechanics analysis device for piezomodulated and sensed single cell, comprising a piezoelectric chip, a detecting tank, and a controlling and detecting system. The mechanics analysis device is characterized in that the piezoelectric chip is encapsulated in the detecting tank; the mechanic dynamic analysis on the piezomodulated and sensed single cell is carried out by controlling an detecting system; the piezoelectric chip consists of a piezoelectric sheet and at least one group of electrodes arranged on the surface of the piezoelectric sheet; one group of electrode pairs and the piezoelectric sheet form a piezoelectric resonator; the electrode pair comprises at least one micro-electrode with the area matched with that of the single cell and surface decorated chemically; the single cell is naturally fixed on the chemically decorated micro-electrode in a bonding way; the microelectrode can generate vibration by the controlling and detecting system and can interact with the bonding cell, thus synchronously realizing the dynamic modulation and real-time induction of the single cell mechanics. The device and the method aim at solving the problem of difficulty on modulation and monitoring of the cell mechanics in the single cell mechanics technology and have the characteristics of no existence of non-mechanic interference, high simpleness and flexibility, high flux and the like; the bio-mechanic characteristics of the cell can be discovered on cell layer, time and space; and the mechanics analysis device can be suitable for application in the wide fieldssuch as biomechanics, tissue engineering, drug screening, clinical medicine, and the like.

The invention discloses a spring nanowire detector for cell mechanics detection. The spring nanowire detector comprises a vertical three-dimensional nanowire spring or a horizontal wavy nanowire typenanowire spring of which one end is fixed on a micromechanical operation platform, and the tail end of the other end of the vertical three-dimensional nanowire spring or the horizontal wavy nanowire type nanowire spring is of a linear or hook-shaped suspended structure; and when the tail end of the other end of the vertical three-dimensional nanowire spring or the horizontal wavy nanowire spring is linear, a fluorescent marker is arranged on the surface of the tail end of the linear nanowire. Related mechanical properties can be tested by controlling the real-time deformation observation of the nanowire spring, and the suspended nanoscale probe is arranged at the end part so that cell damage-free detection can be realized.

The invention belongs to the technical field of biomechanics, and relates to a device and a method for researching cell mechanics. A device for applying an external force to living cells comprises: afirst chip and a second chip, wherein the first chip is provided with a cell culture cavity and a first flow channel communicated with the cell culture cavity; and the second chip is positioned abovethe first chip and used for applying pressure to the first chip. The device can realize cell culture, apply normal and / or tangential forces to the cells in culture, and enrich experimental means forcell mechanics research.

The invention provides a mechanical dynamic regulation and control chip for a single cell total environment. The chip provided by the invention comprises a micro-fluid control layer, a piezoelectric vibration layer and at least one single cell pool and is characterized in that the micro-fluid control layer and the piezoelectric vibration layer are integrated on the chip; the mechanical dynamic regulation and control of a fluid medium with single cells is carried out by regulating the direction and flow velocity of the micro-fluid of the chip; and simultaneously, the mechanical dynamic regulation and control of an extracellular matrix with single cells is carried out by regulating the amplitude and frequency of the piezoelectric vibration of the chip, thereby simultaneously realizing the mechanical dynamic regulation and control of all environments with single cells on the chip. The chip provided by the invention aims to solve the problem of difficulty in dynamic regulation and controlof an environment in a single cell mechanics technique, does not have mechanical dynamic regulation dead zones, has the characteristics of simplicity, convenience and flexibility in operation and high flux, can be used for discovering the biomechanics characteristics of cells in time and space and is suitable for being applied in wide fields such as biomechanics, tissue engineering, drug screening, clinical medicine and the like.

The invention discloses an automatic measurement system and method for mechanical properties of cells, wherein the system comprises a micro-fluidic chip, an industrial personal computer, an image acquisition module, a first micro-flow pump, a second micro-flow pump and a signal generator, and the micro-fluidic chip is respectively connected with the first micro-flow pump, the second micro-flow pump and the signal generator; and the image acquisition module, the first micro-flow pump, the second micro-flow pump and the signal generator are all connected with the industrial personal computer. The invention further discloses an automatic measurement method for the mechanical properties of the cells. Hardware control is carried out through visual feedback, the cell length is recognized in combination with an image morphology algorithm, large-batch cell deformation quantity data is automatically measured, a cell mechanical database is established, cell mechanical characteristic analysis has statistical significance, operation is easy, the automation degree is high, the efficiency is high, external system integration can be carried out on various micro-fluidic chips or other similar devices integrating single cell capturing, stretching and releasing functions, the application range is wide, and the applicability is high.

The invention discloses an erythrocyte mechanical property measuring device and method. The erythrocyte mechanical property measuring device comprises an injection pump, a power-adjustable signal generator, a surface acoustic wave driving array module, a temperature control module, a microscope, a CCD camera and a computer. The surface acoustic wave driving array module comprises a piezoelectric substrate, a first interdigital transducer, a second interdigital transducer, a third interdigital transducer, a fourth interdigital transducer, a fifth interdigital transducer, a sixth interdigital transducer and a micro-channel bonded on the piezoelectric substrate, wherein the first interdigital transducer, the second interdigital transducer, the third interdigital transducer, the fourth interdigital transducer, the fifth interdigital transducer and the sixth interdigital transducer are deposited on the piezoelectric substrate. The piezoelectric substrate is tightly attached to the upper surface of the temperature control module. Erythrocytes flowing into the micro-channel are deformed under the drive of acoustic surface waves generated by the interdigital transducer, and mechanical properties are calculated by using a mathematical model by analyzing erythrocyte deformation parameters shot by the CCD camera. The device and the method can be used for measuring the mechanical propertyof a single red blood cell with the particle size of less than 10 microns and simultaneously reflecting the elastic mechanical property and the tension property.