Mechanics analysis device and mechanics analysis method for piezomodulated and sensed single cell

Abstract

The invention provides a mechanics analysis device for piezomodulated and sensed single cell, comprising a piezoelectric chip, a detecting tank, and a controlling and detecting system. The mechanics analysis device is characterized in that the piezoelectric chip is encapsulated in the detecting tank; the mechanic dynamic analysis on the piezomodulated and sensed single cell is carried out by controlling an detecting system; the piezoelectric chip consists of a piezoelectric sheet and at least one group of electrodes arranged on the surface of the piezoelectric sheet; one group of electrode pairs and the piezoelectric sheet form a piezoelectric resonator; the electrode pair comprises at least one micro-electrode with the area matched with that of the single cell and surface decorated chemically; the single cell is naturally fixed on the chemically decorated micro-electrode in a bonding way; the microelectrode can generate vibration by the controlling and detecting system and can interact with the bonding cell, thus synchronously realizing the dynamic modulation and real-time induction of the single cell mechanics. The device and the method aim at solving the problem of difficulty on modulation and monitoring of the cell mechanics in the single cell mechanics technology and have the characteristics of no existence of non-mechanic interference, high simpleness and flexibility, high flux and the like; the bio-mechanic characteristics of the cell can be discovered on cell layer, time and space; and the mechanics analysis device can be suitable for application in the wide fieldssuch as biomechanics, tissue engineering, drug screening, clinical medicine, and the like.

Description

technical field [0001] The invention relates to the technical field of biological analysis, and is a chip for dynamic analysis of single cell mechanics. Background technique [0002] As the basic unit of life, cells constantly interact with their environment (including extracellular matrix and media). It has an important impact on life activities such as apoptosis and migration. On the other hand, an important manifestation of cell activity is that cells produce mechanical changes, and the mechanical changes of cells further regulate cell activities. Therefore, how cells sense the chemical and mechanical stimuli of the environment and convert them into mechanical signals and regulate cell activities, that is, cell mechanics, constitutes one of the basic issues of life activities. The development of cell mechanics technology as a supporting platform promotes the deepening of understanding of this fundamental problem and plays an increasingly important role in improving huma...

AI Technical Summary

Problems solved by technology

For precision detection methods represented by AFM and magnetic torsion measurement, mechanical probes need to be fixed on the cell surface, and the introduction of non-mechanical effects will cause deviations in mechanical detection

And more importantly, the existing technology has sophisticated devices and complicated methods, and it is difficult to achieve high-throughput cell mechanical analysis, which restricts its application in clinical diagnosis, drug screening and other fields.

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