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Device and method for applying external force to living cells and high-flux microfluidic chip

A technique for applying external force and living cells, applied in the field of biomechanics

Pending Publication Date: 2020-01-21
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing methods and instruments are generally only used to exert a single direction and type of external force on cells, such as tangential fluid shear force or normal pressure, etc.

Method used

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  • Device and method for applying external force to living cells and high-flux microfluidic chip
  • Device and method for applying external force to living cells and high-flux microfluidic chip
  • Device and method for applying external force to living cells and high-flux microfluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 This example provides a device for applying normal and tangential external forces to living cells, such as figure 1 , 2 , 3, mainly composed of a first chip 101, a second chip 102 and a glass slide 103. The first chip 101 is bonded on the glass slide 103 , and the second chip 102 is located above the first chip 101 . The first chip and the second chip are made of polydimethylsiloxane (PDMS). The PDMS chamber coated with basal protein is suitable for the cultivation of various cells, and the cells and medium can be injected and replaced through microchannels.

[0037] Such as figure 2 As shown, the first chip 101 is provided with a first flow channel 11, and five branches 12 are evenly distributed on the trunk of the first flow channel 11. As a preferred method, the five branches are perpendicular to the trunk of the first flow channel 11 and parallel Distributed on the same side of the first flow channel 11. Five cell culture chambers 13 are respectively...

Embodiment 2

[0044] Example 2 This example provides a method for applying normal and tangential external forces to living cells, which specifically includes the following steps:

[0045] (1) The cell solution is injected into the first flow channel 11 through the inlet pipe 14 , and then distributed to the cell culture chamber 13 on the five branches, and the excess liquid is discharged through the outlet pipe 15 .

[0046] (2) After the injected cells grow normally attached to the wall, inject liquid into the flow channel f21 and the flow channel g22. After the flow channel is filled with liquid, it will be compressed, and pressure can be applied to the branch 12 of the first flow channel 11 to block the first flow channel. Branches, so that the flow channels at both ends of the cell culture chamber 13 are closed; the cells grow normally in a relatively stable environment.

[0047] (3) Inject liquid into the pressure chamber 26 through the third channel 25, and use the hydraulic pressure ...

Embodiment 3

[0051] Example 3 This example provides a high-throughput microfluidic chip with a structure such as Figure 9 As shown, several devices as in Embodiment 1 are distributed on the chip in an array. There are a total of 12 devices on the chip with a size of 75mm X 25mm, and each device can set different external force conditions. Each device is equipped with 5 parallel cell culture chambers, which can be used for statistical analysis of the same conditions. The specification (length X width) of each cell culture chamber is 500μm X 500μm, which can accommodate multiple cells.

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PUM

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Abstract

The invention belongs to the technical field of biomechanics, and relates to a device and a method for researching cell mechanics. A device for applying an external force to living cells comprises: afirst chip and a second chip, wherein the first chip is provided with a cell culture cavity and a first flow channel communicated with the cell culture cavity; and the second chip is positioned abovethe first chip and used for applying pressure to the first chip. The device can realize cell culture, apply normal and / or tangential forces to the cells in culture, and enrich experimental means forcell mechanics research.

Description

technical field [0001] The invention belongs to the technical field of biomechanics, and relates to a device and a method for studying cell mechanics. Background technique [0002] Cell mechanics is a frontier field of biomechanics and an important part of tissue engineering. It involves the research on the mechanical properties of cells such as cell membrane and cytoskeleton deformation, elastic constants, viscoelasticity, and adhesion under mechanical loads, as well as the influence of mechanical factors on cell growth, development, maturation, proliferation, aging, and death. its mechanism research. [0003] For cell mechanics, the prerequisite for its research is the mechanical loading method of cells; therefore, finding a suitable cell mechanical loading method and related biological measurement methods is the primary issue in the study of cell mechanics. Cell mechanics experiment is a simulation of the biomechanical environment in which cells live under certain condi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M3/00
CPCC12M35/04C12M23/16
Inventor 韩琳邵晓薇王敏刘宏张宇褚玉金
Owner SHANDONG UNIV
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