53 results about "Cell deformation" patented technology

A method for drying a honeycomb formed body using microwaves is provided. The method can reduce the difference in the drying speed inside the honeycomb formed bodies during the drying process and can dry the honeycomb formed bodies without cell deformation. The method comprises placing the honeycomb formed body in a drying furnace with the cell axis in the vertical direction and irradiating the honeycomb with microwaves at a frequency of 300-30,000 MHz. The difference of the water content in the vertical direction of the honeycomb formed body 1 is maintained at 0.3% per mm or less during microwave irradiation.

The invention discloses a lithium ion battery, a coating separator and a preparation method thereof, comprising: a separator base material, wherein the separator base material is a porous polymer filmlayer; A first coating uniformly and continuously formed on at least one surface of the diaphragm substrate; A second coating layer dispersed at a predetermined pitch on the surface of the first coating layer or the surface of the diaphragm substrate by a conical deposit having a preset stacking shape. The first coating and the second coating of the coating diaphragm of the invention increase thebarrier action of the diaphragm between the positive electrode and the negative electrode, and reduce the probability of short circuit in the positive electrode and the negative electrode. The firstcoating also has good thermal stability to the diaphragm substrate. The second coating is sprayed by atomization, the cone-shaped deposit has little effect on the permeability of the membrane, and thegap provides a better channel for the flow and infiltration of the electrolyte. The second coating also possesses cold/hot pressing irreversible deformation and good adhesion, which reduces the riskof cell deformation and improves the electrochemical performance of the battery.

The invention provides a preparation method of a high temperature-resistant intelligent energy absorbing material. A damping and protecting material with a high buffering performance is prepared with energy absorbing gel as a filler and a silicone rubber foamed material as a base body. The energy absorbing gel spontaneously becomes hard from soft when being impacted, and absorbs energy in the becoming process in order to realize an effective protection effect, and the silicone rubber foamed material used as the structure base body also can absorb parts of impact energy through cell deformation when being acted, so the base body and the filler are simultaneously used to realize damping and buffering effects.

The invention discloses a method for fixing a fish brain tissue specimen through systemic heart perfusion. The method is mainly characterized in that after fish anesthesia, thoracotomy is performed on the periphery of a heart surface projection area of the fish, ventral aorta intubation is performed through the ventricle, common cardinal vein intubation is performed through the atrium, ligation with an operation thread is used for fixing, and an out flow tube is connected with a graduated vessel for measuring liquid out-flowing quantity; a constant-speed perfusion pump is used for perfusing normal saline through a perfusion tube, and a 10% formalin solution is adopted for reperfusion; when perfusion is started, a line is marked on the skull instantly and craniotomy is performed, and the perfusion time is then decided through combination of the perfusion quantity and the out-flowing quantity when the color of the brain tissue changes white; the brain is picked and placed in the 10% formalin solution to be fixed for 48 h after perfusion is stopped, and the brain tissue specimen with complete structure, normal form and good state is obtained. By means of the method, the problems of insufficient fixing, non-comprehensive fixing area, tissue autolysis, cell deformation and the like of the fish brain tissue can be solved effectively, the brain tissue specimen with more complete structure and more ideal state is obtained easily, and the natural form is retained better.

A method for drying a honeycomb formed body using microwaves is provided. The method can reduce the difference in the drying speed inside the honeycomb formed bodies during the drying process and can dry the honeycomb formed bodies without cell deformation. The method comprises placing the honeycomb formed body in a drying furnace with the cell axis in the vertical direction and irradiating the honeycomb with microwaves at a frequency of 300-30,000 MHz. The difference of the water content in the vertical direction of the honeycomb formed body 1 is maintained at 0.3% per mm or less during microwave irradiation.

A polystyrene resin extruded foam plate is obtained by extruding a foamable molten resin mixture comprising a mixed blowing agent containing the isobutane (except a chlorofluorocarbon and/or a fluorocarbon) and a polystyrene resin from a high-pressure zone to a low-pressure zone and has >=10 mm thickness and 25-60 kg/m3 apparent density. The residual amount of the isobutane in the foam plate is 0.45-0.80 mol based on 1 kg of the foam plate. Furthermore, the foam plate has 0.05-0.18 mm average cell diameter in the thickness direction and 0.7-1.2 cell deformation ratio (the average cell diameter in the thickness direction/the average cell diameter in the horizontal direction) and satisfies type 3 of the flammability standard of the polystyrene extruded foam heat insulating plate described in JIS A9511(1995) and further has <=0.028 W/mK thermal conductivity of the foamed plate.

A coating comprising a metal-organic framework, wherein the metal-organic framework having a zeolitic structure comprising at least one multivalent metal species and at least one organic ligand (such as zeolitic imidazolate framework (ZIF)). Said coating has a topography comprising an array of projections, and each projection having at least one tapered distal end. There is also provided a method of coating substrates with the disclosed coating and use of said coating as a disinfectant, an antiseptic, or an antibiotic. Such use is possible because the tapered distal end of the disclosed zeolitic structure exerting higher pressure on any microbial cell that comes into contact with the disclosed coating, thereby piercing through the cell membrane more easily, causing cell deformation and lysis.

One embodiment of the present invention provides a system that measures single cell mechanics using a scanning probe microscope. During operation, the system positions a modified probe of the scanning probe microscope above a cell which is located on a surface, wherein the modified probe is configured with a geometry for compressing the cell. The system then comprises the cell against the surface using the modified probe, thereby causing the cell to deform. Next, the system extracts mechanical properties of the cell from cell deformation behavior and cell response to the compression force.

The invention provides a method suitable for in-situ hybridization of a cherax quadricariratus gonadal tissue mRNA paraffin section, and belongs to the technical field of in-situ hybridization. The method comprises the steps of gonadal tissue embedding, paraffin sectioning, in-situ hybridization, and photographing and recording. The sequence of a probe used in in-situ hybridization is as shown inSEQ ID NO.3. The probe has clear specificity on tissue positioning of a dsx gene, so that the display effect on the mRNA level of the dsx gene is realized. The method for mRNA in-situ hybridization ofthe paraffin section is established in cherax quadricariratus to research expression modes of related genes in the gonad, and the expression location of the sex-related genes of the cherax quadricariratus in the gonad can be clearly described. The method avoids tissue shrinkage and cell deformation while the dewaxing effect of the paraffin section is good, so that the tissue is still kept in theoriginal position, the tissue morphology is kept complete, the enzymolysis effect can be improved, cell coloring is good, nucleoplasm is clear, and the in-situ hybridization accuracy is finally improved.

The present invention relates to a substrate comprising a plurality of integrally formed surface features, said surface features being micro-sized and/or nano-sized, said surface features comprising at least one pointed terminus. As a result of this unique surface, said substrate exhibits a biocidal activity because the terminal ends of said surface feature pierce through cell membrane of any microbial cell that comes into contact with the substrate, thereby causing cell deformation and lysis. The present invention also relates to a method producing said substrate. By a simple treatment of copper or zinc foil with a reagent solution comprising an alkali and an oxidizing agent, Cu(OH)2 nanotube arrays, CuO nano-blades and ZnO nano-needles are prepared. These surfaces are proven to be very effective in killing bacterial (such as E. coli) via a physical interaction.

The invention discloses an indirect coupling simulation method of a microfluidic dielectrophoresis sorting chip, and relates to the technical field of dielectrophoresis. The method comprises the following steps: establishing a phase field model in a multiphase flow; adopting a finite element method to obtain dielectrophoretic force which changes along with time and space and is borne by particles;inputting the dielectrophoresis force as an external force item into the phase field model in the multiphase flow, and solving the phase field model in the multiphase flow to realize indirect coupling; adopting a lattice Boltzmann method to calculate the phase field model in the multiphase flow, calculating electric field intensity distribution in a chip and track and characterize motion trails of the particles. The advantages of the finite element grid method for solving the electric field can be exerted, the advantages of the lattice Boltzmann method for solving micro-scale flow and flexible cell deformation can also be exerted, the chip structure can be optimized according to the simulation result, and a new thought is provided for design and manufacturing of the microfluidic dielectrophoresis chip.

The invention discloses a device and a method for testing mechanical properties of biological cells by acoustic radiation force, and the device comprises an ultrasonic module, a test module and an analysis module; the test module comprises an inverted biological microscope, a cell culture dish is movably placed on the operation platform, an irradiation light source is arranged above the perpendicular bisector of the operation platform from top to bottom, and a lens cone connected with the analysis module is arranged below the operation platform; the ultrasonic module is connected with the ultrasonic focusing transducer; a probe at the front end of the ultrasonic focusing transducer is obliquely immersed into the culture dish from the upper part of the operation platform; the analysis module acquires images from the lens cone and realizes real-time dynamic acquisition and processing of image signals; an ultrasonic focusing transducer is driven by an ultrasonic module, acoustic radiation force is generated, target cells in a cell culture dish are captured, force is applied to the target cells, a cell deformation image is captured and extracted by an analysis module, a cell boundary diagram is drawn, and then the cell deformation quantity is calculated.

The invention discloses a method for nanotube-height-aided control of cytoskeleton change. The method comprises steps of performing anodization of pure titanium or low-modulus biphase titanium using an organic solvent; representing the microscopic structure of an anodization product using a microscopic means; preparing a titanium metal piece of nanopore and naotube microstructures; separating bone narrow mesenchymal stem cells, adhering three-generation cells on a surface-modified metal piece for incubation; observing cell adhesion effects by MTT, DYPI nuclear staining and SEM; placing the metal testing piece for cell incubation in culture holes of an aseptic elastic plate, and placing the metal testing piece in a universal testing system in a constant temperature state and providing the metal testing piece with different periods, frequencies and load compressive stress; and observing and testing cell deformation with a SEM electron microscope or optical microscope, and detecting representation of cell proliferation and differentiation. The method enables the elastic deformation signal of a modified titanium substrate surface to be amplified, thereby influencing osteoblast adhered on the modified titanium substrate surface to receive greater simulation, and leading to larger cytoskeleton change.

The invention relates to a high-flux microfluidic system for researching mechanical and biochemical signal induced single cell dynamic response and a use method thereof, and belongs to the field of cell biology experimental devices. The system comprises a micro-fluidic chip and a peripheral loading, detection and control device. The loading device is combined with the control device, so that a large number of single cells can be captured or controlled, and different dynamic mechanical and biochemical stimulation signals can be accurately loaded to the cells. The control device receives dynamic image information such as single cell deformation, motion trail and biochemical signal space-time distribution observed by the detection device in real time and pressure/flow value in the loading device, so as to feed back and control the loading device, control the single cell deformation process and accurately control the micro-channel flow field and biochemical signal transmission. The dynamic mechanics and biochemical microenvironment of in-vivo cells can be accurately simulated. According to the invention, efficient capture and control of a large number of single cells can be realized, and the method can be used for cell biology research such as analysis of dynamic response and mechanism of the single cells under stimulation of different dynamic mechanical and biochemical signals.