High-flux micro-fluidic system for researching mechanical and biochemical signal induced single cell dynamic response and use method thereof

Abstract

The invention relates to a high-flux microfluidic system for researching mechanical and biochemical signal induced single cell dynamic response and a use method thereof, and belongs to the field of cell biology experimental devices. The system comprises a micro-fluidic chip and a peripheral loading, detection and control device. The loading device is combined with the control device, so that a large number of single cells can be captured or controlled, and different dynamic mechanical and biochemical stimulation signals can be accurately loaded to the cells. The control device receives dynamic image information such as single cell deformation, motion trail and biochemical signal space-time distribution observed by the detection device in real time and pressure / flow value in the loading device, so as to feed back and control the loading device, control the single cell deformation process and accurately control the micro-channel flow field and biochemical signal transmission. The dynamic mechanics and biochemical microenvironment of in-vivo cells can be accurately simulated. According to the invention, efficient capture and control of a large number of single cells can be realized, and the method can be used for cell biology research such as analysis of dynamic response and mechanism of the single cells under stimulation of different dynamic mechanical and biochemical signals.

Description

technical field [0001] The invention belongs to the field of cell biology experimental devices, and is composed of a microfluidic chip and peripheral loading, detection and control devices designed based on fluid mechanics and microfluidic chip technology. Dynamically responsive high-throughput microfluidic systems. Background technique [0002] Somatic cells exist in a complex dynamic flow microenvironment composed of surrounding tissue cells, interstitial cells and body fluids. Cells are not only stimulated by mechanical signals such as fluid shear force, positive pressure, and tensile force in the microenvironment, but also stimulated by the microenvironment. Co-stimulation of biochemical factors, hormones, neurotransmitters and other biochemical substances concentration signals. Cells can recognize mechanical and biochemical signals from the extracellular microenvironment, transduce and transmit signals to the interior of the cell through various signaling pathways, res...

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Problems solved by technology

Existing high-throughput microfluidic chips for dynamic analysis of single cells are mainly divided into two categories: one is flow cytometry, which allows single cells to squeeze through a narrow single channel with fluid based on the principle of flow cytometry, and High-throughput manipulation of single cells is achieved by increasing the fluid velocity, which is very suitable for high-throughput detection of single-cell stress deformation, but it is mainly used for mechanical signal loading of single cells, lacking research under the co-stimulation of biochemical factors; another type It is a microarray type, which performs high-throughput capture of single cells based on microstructure arrays, and realizes dynamic biochemical signal loading by controlling the flow field and pressure distribution outside the single cell or controlling the transmission of biochemical signals. Chips are more suitable for high-throughput detection of single-cell dynamics, but most of them are difficult to achieve accurate quantitative loading of signals

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