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Soap embedded plant section method

A plant slice and soap technology, applied in the field of biological microscopy, can solve the problems of xylene harmful to human health, complicated operation, large cell deformation, etc., and achieve the effect of low cost of slice production, less processing steps, and small cell deformation

Inactive Publication Date: 2010-06-30
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects of the traditional paraffin sectioning method, such as slow speed, complex operation, large cell deformation, high cost, difficulty in obtaining thin slices by freehand sectioning, and xylene endangering human health, etc., and provide a method for slicing plants embedded in soap , this method uses soap as the embedding material, which overcomes the disadvantages of making paraffin sections, and has broad application prospects in the field of botanical research.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Cut the branches of Quercus gooseberry into sections of about 0.8cm, trim the embedding material soap into a square of about 2×1.5×0.8cm, make an incision in the middle of one end of the square, put the branches of Quercus gooseberry into the cut, drip water through the cut, cut After smoothing and pressing the incision, put it in the refrigerator at -5°C for 2 hours, and then take out the slices.

Embodiment 2

[0025] Cut the leaves of Rhododendron twist into pieces of about 0.8cm×0.6cm, trim the embedding material soap into a square of about 2×1.5×0.8cm, make an incision in the middle of one end of the square, put the leaves of Rhododendron twist into the cut, drip water through the cut, After smoothing the incision and pressing the incision tightly, put it in the refrigerator at -10°C for 3 hours, and then take out the slices.

Embodiment 3

[0027] Cut the root of the white thorn tree into sections of about 0.8 cm, trim the embedding material soap into a square of about 2×1.5×0.8 cm, make an incision in the middle of one end of the square, put the root of the white thorn tree into the cut, and drip water through the cut , Smooth the incision, press the incision tightly, put it in the refrigerator at -18°C for 4 hours, and then take out the slices.

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PUM

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Abstract

The invention relates to a soap embedded plant section method which adopts soap as embedding material, and overcomes the defects that the traditional paraffin section method is slow in speed, complicated in operation, large in cell deformation, expensive in expense, hard in obtaining a thin slice by hands, harmful for human health due to dimethylbenzene, and the like. The method has the advantages of short manufacturing period generally needing about five hours, fewer steps for the treatment of herbarium, small deformation of section preparation cells, low cost in manufacture of the slice, no hurt for the body of an experimenter, no environmental pollution, etc.

Description

technical field [0001] The invention relates to the technical field of biological microscopy, in particular to a method for embedding plant slices in soap. Background technique [0002] In the field of plant anatomy research, plant microscopic techniques generally adopt paraffin section biotechnology, which has obvious shortcomings: [0003] ①Long production cycle: The entire production process of conventional paraffin sections generally takes at least 4 days; [0004] ②There are many processing steps for plant specimens: conventional paraffin sections have many physical and chemical processing steps for plant materials and sliced ​​specimens: [0005] Material washing → dehydration treatment (including 30%, 50%, 70%, 80%, 90%, 100% alcohol repeated three times; half an hour each time) → dealcoholization and transparency (including 1 / 2 xylene + 100% alcohol Treat once, pure xylene 2 times; half an hour each time) → wax dipping and embedding (1 / 2 paraffin + 1 / 2 xylene → pur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 陈红
Owner SOUTHWEST UNIVERSITY
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