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A cancer cell separation device based on cell deformation quantity and dielectrophoresis force and a control system

A separation device and dielectrophoretic force technology, applied in the field of cell sorting and separation, can solve problems such as inability to separate cells

Active Publication Date: 2019-01-01
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Despite the advantages of dielectrophoretic force-based separation methods, they cannot separate cells of specific interest based on markers

Method used

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  • A cancer cell separation device based on cell deformation quantity and dielectrophoresis force and a control system
  • A cancer cell separation device based on cell deformation quantity and dielectrophoresis force and a control system
  • A cancer cell separation device based on cell deformation quantity and dielectrophoresis force and a control system

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preparation example Construction

[0047] The fabrication process of the microchannels is as follows: the microchannels are created on top of the electrodes by building a 20 μm thick layer of SU-8 using standard photolithography techniques.

[0048] On one side of the inner wall of the microchannel, there are a plurality of electrodes with a tooth-like structure embedded, and the positive and negative electrodes are intersected to generate dielectrophoretic force in the microchannel; the dielectrophoretic force generated by the electrodes acts on the microchannel amebocytes.

[0049] The electrode on the wall of the microchannel of the cancer cell separation chip 5 is platinum metal, and platinum is a kind of inert metal, which itself does not participate in the electrode reaction when it is used as an electrode.

[0050] In order not to damage the cells, a porous hydrogel layer was laid on the platinum electrodes, which can avoid the contact between the deformed cells and the electrodes.

[0051] The electrod...

Embodiment 2

[0057] An embodiment of the present invention provides a cancer cell separation control system based on cell deformation. The cancer cell separation control system is applied to the device provided in Embodiment 1. The cancer cell separation control system includes: the CMOS high-speed camera 9 It is used to capture the image of the deformed cells in the cell deformation detection chip 8 .

[0058] The processor 11 includes a processing module and a judging module.

[0059] The processing module is connected with the CMOS high-speed camera 9, and is used for acquiring the image of the amebocyte, and processing the image of the amebocyte to determine the amount of deformation of the amebocyte.

[0060] The judging module, connected to the electrode, is used to judge whether the deformation of the deformed cells is greater than a preset threshold stored in the processor, and when the deformation of the deformed cells is greater than the preset threshold, When the drive signal i...

Embodiment 3

[0062] The embodiment of the present invention provides a method for measuring the deformation of cancer cells and separating them based on dielectrophoretic force technology. The specific implementation steps are as follows:

[0063] a. Preparation of cells: prepare the blood samples to be tested in advance, centrifuge the samples at 115g for 5min, and then resuspend them in phosphate buffered saline (PBS) so that the final concentration of the cells is 10 6 cells / mL, only 100 μL of cell suspension is required for each detection. Prepare an isotonic solution so that the conductivity of the cell suspension is 0.55 S / m during the experiment. Before aspirating the cell suspension from the cell reservoir 7 into 1 mL of the syringe pump 1, the cell suspension was placed at 37° C. to maintain cell viability.

[0064] b. Experimental device preparation: thoroughly clean the channels of the syringe micropump 1, the polymer tube 2, the cell deformation detection chip 8, and the cell ...

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Abstract

A cancer cell separation device based on cell deformation quantity and dielectrophoresis force and a control system are disclosed. The device includes a cell reservoir and an injection micropump. Oneend of the injection micropump extends into the cell reservoir and the other end is connected to an inlet end of a cell deformation quantity detection chip. An outlet end of the cell deformation quantity detection chip is connected to a deformed cell inlet end of a cancer cell separation chip. A CMOS high-speed camera is disposed below the cell deformation quantity detection chip to capture deformed cell images. A processor determines the deformation quantity of a deformed cell according to an acquired deformed cell image, and determines whether the deformed cell is a cancer cell or not according to the deformation quantity, and if yes, the processor outputs a drive signal to drive an electrode to generate dielectrophoresis force to allow the deformed cell to flow towards a cancer cell outlet end of the cancer cell separation chip. Through the device and the system, deformation quantity of cells can be detected rapidly, and cancer cells can be separated from blood samples of patients according to the difference in deformation quantity between the cancer cells and normal cells.

Description

technical field [0001] The invention relates to the technical field of cell sorting and separation, in particular to a cancer cell separation device and control system based on cell deformation and dielectrophoretic force. Background technique [0002] Cancer is a major disease that seriously endangers human health, and its morbidity and mortality are increasing year by year. For cells of the same origin, cancerous cells are softer than normal cells, are more likely to deform, and are more prone to cancer metastasis. Mechanophenotype has long been considered a label-free method to quantify functional changes in cells of physiological and pathological origin. This approach has been validated in the context of cell differentiation and malignant metastasis and has led to the development of many basic research methods. Among them, the change of the deformability of single cells can be used as an important indicator of biological research. Compared with biomolecular markers, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N15/14
Inventor 柯俊杜盼盼刘娜刘媛媛谢少荣杨毅
Owner SHANGHAI UNIV
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