Device and method for testing mechanical properties of biological cells by using acoustic radiation force

Abstract

The invention discloses a device and a method for testing mechanical properties of biological cells by acoustic radiation force, and the device comprises an ultrasonic module, a test module and an analysis module; the test module comprises an inverted biological microscope, a cell culture dish is movably placed on the operation platform, an irradiation light source is arranged above the perpendicular bisector of the operation platform from top to bottom, and a lens cone connected with the analysis module is arranged below the operation platform; the ultrasonic module is connected with the ultrasonic focusing transducer; a probe at the front end of the ultrasonic focusing transducer is obliquely immersed into the culture dish from the upper part of the operation platform; the analysis module acquires images from the lens cone and realizes real-time dynamic acquisition and processing of image signals; an ultrasonic focusing transducer is driven by an ultrasonic module, acoustic radiation force is generated, target cells in a cell culture dish are captured, force is applied to the target cells, a cell deformation image is captured and extracted by an analysis module, a cell boundary diagram is drawn, and then the cell deformation quantity is calculated.

Description

technical field [0001] The invention relates to the technical field of cell mechanics testing, and mainly relates to a device and a testing method for testing the mechanical properties of biological cells by using acoustic radiation force. Background technique [0002] The study of the mechanical properties of biological cells has become a new research hotspot in the field of acoustics in recent years, especially in the study of the mechanical properties of cancer cells, because changes in the mechanical properties of cells are often closely related to the occurrence of related diseases, so a single The study of cell mechanical properties may detect and identify a small number of abnormal cells and provide a potential method for early diagnosis of malignant diseases, so this is a promising research project. In the previous study of cell mechanical properties, several methods such as micropipette method, atomic force microscope (AFM), optical tweezers and magnetic tweezers we...

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Problems solved by technology

This method can only realize the quantitative detection of the deformability of the adherent cells, but cannot realize the automatic capture and force deformation detection of the suspended biological cells, and the optical mechanothermal effect is obviously easy to damage the cells.

[0004] In 2016, JaeYoun Hwang et al. introduced ultrasonic transducers into the test of cell mechanical properties in the article Cell Deformation by Single-beam Acoustic Trapping: A Promising Tool for Measurements of CellMechanics in Scientificreports. In the scheme, the transducer probe adopts vertical from top Placement, the probe and the mercury lamp light source are respectively located on the upper and lower sides of the cell operation platform. The illumination light path is built by itself, using a bottom-up method, and the instruments and equipment need to be built with the help of an optical operation platform. This solution is costly, large in size, and cumbersome in structure and more complex

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