35 results about "Magnetic tweezers" patented technology

The invention discloses a single molecule control transverse magnetic tweezer device, comprising: microscopic imaging device, magnetic tweeze device, sample holder and central monitor, where the magnetic tweezer device comprises magnetic tweezer and magnetic tweezer controller, the magnetic tweezer is horizontally arranged on the side surface of the sample tank and controlled by the magnetic tweezer controller; the sample tank is a transparent closed container, the two ends of the sample tank are provided with solution inlet and outlet, respectively, one end of a biological single molecule is connected with the side wall of the transparent container far from the magnetic tweezer and the other end is connected with a paramagnetic ball, which is controlled by the magnetic tweezer; the microscopic imaging device is arranged at the upper or lower part of the sample tank, and the microscopic imaging device and the sample tank can horizontally and relatively translate; the microscopic imaging device transmits the obtained image information to the central monitor for data processing. And its beneficial effects: it can research and analyze biological single molecule, including related enzyme and protein, etc, and it can directly give length information of the biological single molecule and it can rotate the biological single molecule.

The invention belongs to the technical field of microimaging, and discloses a magnetic tweezer device for controlling the motion of single molecule. The device comprises a sample pool, a magnet control unit, an imaging unit and a control unit, wherein the sample pool is used for containing a single molecule sample and solution; the single molecule sample is positioned in the solution; one end of the single molecule sample is connected to the bottom part of a piece of cover glass of the sample pool and while the other end of the single molecule sample is connected with a magnetic ball; the magnet control unit is positioned below the sample pool and used for controlling the motion of the magnetic ball; the imaging unit is used for transmitting light to the sample pool and outputting a reflected light signal that records the motion state of the magnetic ball; the control unit is used for receiving and converting the reflected light signal and measuring and analyzing the motion of the magnetic call. According to the magnetic tweezer device, a vertical up-down tweezer is provided, which avoids system error caused by gravity, and the measurement precision is also greatly raised.

The invention discloses a magnetic tweezers and optical tweezers measuring and controlling system, belonging to the technical field of microscopy. The magnetic tweezers and optical tweezers measuring and controlling system comprises a sample movement device, wherein the sample movement device is connected with a sample tank; a laser emission device is used for emitting laser; the laser emitted by the laser emission device is irradiated into the sample tank; the laser is irradiated to microparticles or microspheres by a laser microscopic device; a laser monitoring device is used for receiving the laser emitted by the laser emission device and an image in the sample tank is photographed by a visible light imaging device; a central controller is connected with the laser monitoring device; a central processing unit is connected with the visible light imaging device and is connected with the sample movement device; and the central processing unit is connected with a magnetic tweezers device and is connected with the laser emission device. The magnetic tweezers and optical tweezers measuring and controlling system can be used for simultaneously controlling two different molecules by an optical trap and magnetic tweezers, and has great degree of freedom, great force range and high accuracy.

The invention discloses a single molecule fluorescence apparatus and an application method thereof. The single molecule fluorescence apparatus comprises a lens-type full internal reference fluorescence microscope system, an image acquisition and processing system, a controllable magnetic tweezer system and a fluid pool system. The fluid pool system comprises a fluid pool fixed station, a fluid pool and an electric liquid flow pump; one side of the fluid pool fixed station is fixed on a sample stage of an inverted microscope of the full internal reference fluorescence microscope system, and the other side of the fluid pool fixed station is fixed with the fluid pool. The electric liquid flow pump and the fluid pool are connected; and the bottom of the fluid pool is equipped with more than one column of DNA fixed points. Columns of DNA fixed points are arranged in the fluid pool, so controllable fixing of a large amount of DNA can be realized. Through the transverse magnetic tweezer system, controllable drawing on a large amount of DNA molecules can be realized; and high precision observation on protein fluorescence signals can be realized without dyeing on DNA molecules.

The invention discloses an imaging magnetic tweezers device, and a system and a method for integrating imaging magnetic tweezers device with a single-molecule fluorescence technology. The imaging magnetic tweezers device comprises a magnetic object lens with adjustable magnetic field gradient and magnetic field intensity, an illuminating light source and an electric coupling device. The magnetic object lens is combined with the illuminating light source and the electric coupling device to realize superparamagnetic microsphere imaging and conduct single-molecule mechanical control at the same time. On one hand, a magnetic tweezers experiment can be conduct, the magnetic tweezers device can be integrated with various single-molecule fluorescence technologies for application, not only can fluorescence signal detection be conducted to a sample, but also mechanical control can be conducted to the sample, and the fluorescence signal detection and the mechanical control are independent to each other and do not interfere with each other; and on the other hand, the imaging magnetic tweezers device can apply constraints to superparamagnetic microspheres in the sample, the Brownian fluctuation of a single-molecule connecting system can be effectively reduced, an integrated single-molecule fluorescence device can obtain staler fluorescence signals, and the experimental accuracy during single-molecule control measurement is improved.

The invention provides a dual-imaging magnetic tweezer system. The dual-imaging magnetic tweezer system comprises a bearing device, a magnetic tweezer device, a microscopic measuring and controlling device, a microscopic imaging device and a control device, wherein the bearing device bears sample molecules; the magnetic tweezer device performs mechanical manipulation on the sample molecules to enable the sample molecules to move; the microscopic measuring and controlling device collects first image data of the movement of the sample molecules from the side faces of the sample molecules; the microscopic imaging device collects second image data of the movement of the sample molecules from the positions above the sample molecules; the control device analyzes the first image data to obtain a first analysis result, analyzes the second image data to obtain a second analysis result and verifies the first analysis result and the second analysis result to determine a result of the sample molecules changing with time in the three-dimensional space. Accordingly, fluorescence signal detection can be performed on the sample molecules through the microscopic measuring and controlling device, and meanwhile the mechanical manipulation can be performed on the sample molecules through the microscopic imaging device; in addition, verification can be performed according to the obtained first image data and the obtained second image data, and the test precision is improved.

The invention discloses a multi-channel single-protein magnetic tweezer measurement and control method and system, the method comprises the steps of: first preparing a protein sample, identifying a plurality of microbeads to be tracked in a field of view by automatic detection or manual intervention, and simultaneously selecting an appropriate reference microbead; then controlling lifting and rotation of a magnet by a motor to control the sizes of stress and torque of the microbeads in the sample, performing real-time display after correction, measuring the three-dimensional coordinates of themulti-channel microbeads by a microbead positioning and tracking technology, displaying in real time the real-time change of the length of a protein fastening the microbeads through the waveform; storing the corrected real-time displayed sizes of the stress and the torque of the microbeads and the real-time displayed length of the protein, after the plurality of microbeads to be tracked in the field of view are completely collected and processed, changing to a next field of view of the sample, and repeating the above steps to measure the mechanical properties of the molecule of the protein. The method can realize simultaneous fast sampling of a plurality of research objects and realize high-throughput measurement.

The invention provides a temperature-controlled magnetic tweezer device comprising a microscopic imaging apparatus, a magnetic tweezer apparatus, a sample pool, a central monitor, a sample bench and a temperature control apparatus, wherein the magnetic tweezer apparatus comprises a magnetic tweezer and a magnetic tweezer controller, and the magnetic tweezer is horizontally arranged at a side surface of the sample pool and controlled by the magnetic tweezer controller. One end of a biological single molecule is connected to a sidewall away from the magnetic tweezer in the sample pool, and the other end is connected with a paramagnetic magnetic ball which passes through the magnetic tweezer controller. The sample bench is provided with a sample pool groove for accommodating the sample pool, wherein the sample pool is arranged in the sample pool groove, and the sample bench is prepared by heat-conductive materials. According to the invention, a temperature control function of the sample pool is improved, the controller and semiconductor refrigeration sheets, as well as the heat-conductive sample bench and heat dissipation units can be used to heat or cool, and finally a target temperature in the sample pool can be achieved. The sample pool is a nearly integral structure, thereby being simple in manufacturing steps, convenient to clean, capable of repeatable use, material-saving, and good in sealing.

The invention provides a super-resolution and microscopic analysis MOT (magnetic-optical tweezer) device. The device comprises a first OT (optical tweezer), a second OT, a magnetic field, polystyrenemicrobeads and ferromagnetic microbeads, wherein the polystyrene microbeads are captured by the second OT, and the ferromagnetic microbeads are captured by the first OT; and the ferromagnetic microbeads are operated by the magnetic field to form a MT (magnetic tweezer). The magnetic field and an optical field can be controlled in strength and position, motion is non-mechanical motion, and the samemicrobead can be simultaneously captured and operated. The magnetic field generated by the MT is generated by a current coil, and no magnetic influence is produced on other articles during working. Operation of the MT is realized by changing magnitude and direction of input current, no mechanical motion is produced, and the operation accuracy and stability of the MT are greatly improved. The OT is generated by two or more laser, so that the OT is more precise to control.

The invention provides a magnetic sphere coordinate calibration method and device for a magnetic tweezers device. The calibration method comprises the following steps that 1) an initial positions of the center of a diffraction ring image of a magnetic sphere is obtained in a centroid method; and 2) radial light intensity distribution of the diffraction ring image in directions of first and secondcoordinate axes is obtained, the radial light intensity distribution of the diffraction ring image in the direction of the first coordinate axis is cross-related to corresponding mirroring distribution of the image to calibrate the coordinate of the magnetic sphere in the first coordinate axis, and the radial light intensity distribution of the diffraction ring image in the direction of the secondcoordinate axis is cross-related to corresponding mirroring distribution of the image to calibrate the coordinate of the magnetic sphere in the second coordinate axis. Thus, three-dimensional coordinates of the magnetic sphere can be measured accurately by the calibration device, a loop-up table of the magnetic sphere is generated, and thermal drift of the magnetic tweezers device is corrected inreal time on the basis of the loop-up table.

The invention discloses an MOA resistive current and CT capacitive current joint monitoring system which comprises a system host, a pair of soft magnetic tweezers in signal connection with the system host and a current transformer in signal connection with the system host, wherein the a penetrating core of the soft magnetic tweezers is connected to the grounding end of an end screen of a CT sample, the current transformer is connected onto a connecting circuit of an MOA sample in series, and the system host comprises a signal conditioning unit for processing signals received by the soft magnetic tweezers and the current transformer, a data acquiring unit for acquiring an output signal of the signal conditioning unit and a PC processor in both-way communication with the data acquiring unit. The MOA resistive current and CT capacitive current joint monitoring system can simultaneously monitor capacitive current of the capacitive sample and resistive current of a lightning arrester, monitor a specific value of the capacitive current to total current of the lightning arrester in real time and avoid fluctuation of voltage values. The multiple-device joint monitoring can be opened up by improving the joint monitoring system and adding a current signal acquiring channel, the shortcomings existing in an existing single monitoring system are effectively overcome, and the joint monitoring system is suitable for popularization and usage in a electric power system.

The invention relates to a molecular ruler hairpin structure and a method for measuring the spatial scale accuracy of single-molecule magnetic tweezers by using the molecular ruler hairpin structure.The method comprises the following steps: firstly, constructing a hairpin structure, namely a molecular ruler hairpin structure of which the stem part contains a plurality of CCGG sites; fixing the hairpin structure on the surface of a working pool; monitoring the position of magnetic beads in real time by using single-molecule magnetic tweezers; and performing calculation to obtain an accurate length, namely the length between adjacent CCGG sites, of the molecular ruler hairpin structure between two adjacent pause states. Through conversion of length and base logarithm, a length conversion coefficient can be accurately calculated and is compared with a theoretical value of a worm model, so that the spatial scale accuracy of the single-molecule magnetic tweezers is measured. The method canbe used for calibrating the accuracy of precision instruments, and broadens the way for measuring the accuracy of the precision instruments.

The invention discloses a DNA sequencing device which comprises a three-dimensional nanometer movement platform, a probe, a positioning clamp for clamping and fixing the probe, and a comprehensive sequencing layer. The positioning clamp is fixed to the three-dimensional nanometer movement platform. The comprehensive sequencing layer comprises a solid nanopore chip, a liquid pond, a magnetic tweezers system, a power supply, a current measuring device and an electrode. The invention further discloses a DNA sequencing method based on the DNA sequencing device. The defects are overcome that when sequencing is conducted through an existing DNA sequencing device, a large number of DNA single chains are bound, but no single DNA can be captured, and only one end of the DNA single chain can be pulled to move up for sequencing, so DNA in a solution is prone to Brownian movement in the sequencing process; the high-precision DNA single-basic-group sequencing is realized.

The invention discloses a virus single particle separation method based on nano-micro composite spheres. According to the method, a chip containing an array of the nano-micro composite spheres is taken, the nano-micro composite spheres are formed by embedding nano-spheres in nano-pores in micro-spheres, the nano-spheres have virus adsorption capacity, the surfaces of the micro-spheres do not have specific adsorption capacity to viruses, the spheres contain magnetic particles, the viruses in a virus solution are captured by using the nano-spheres on the nano-micro composite spheres to obtain virus-nano-micro sphere composite particles, and the single virus-nano-micro sphere composite particles are separated out, and therefore, single virus particles are separated out. According to the method, a micropipette method, a magnetic tweezers method or a droplet microfluidic method is combined with the nano-micro composite spheres to capture, detect and separate the single virus particles, and the method has the advantages of high efficiency, rapidness and low cost.

The invention relates to a magnetic tweezer structure, and the structure comprises a container body, a first lens, a second lens and a light response part. A light inlet hole and a light outlet hole are formed in the two ends of the container body respectively, the first lens and the second lens are arranged on the side, close to the light inlet hole, in the container body, and the light responsepart is arranged on the side, close to the light outlet hole, in the container body; and the light response part is filled with a noble metal micro-nano particle solution. Incident light irradiates onthe light response part through the light inlet hole, the first lens and the second lens, a small-area magnetic field can be generated on the precious metal micro-nano particle solution filled in thelight response part, and a tiny magnetic object can be captured through the magnetic field.

The invention relates to a measurement device and method for residual capacity after lambda-DNA neutralization. The measurement device comprises a glass base, wherein an open groove for storing a sample solution is formed in one side of the glass base; a front block piece is arranged on one side, corresponding to the open groove, of the glass base; glass microtubes in through connection with the open groove are formed in the position, corresponding to the open groove, of the glass base; platinum wire leads extending outwards from the inside of the open groove are symmetrically arranged at thetwo ends of the open groove; anti-digoxin is attached to the side wall of one side of the open groove. The solution containing lambda-DNA is injected into the open groove of the measurement device through the glass microtubes of the measurement device, so that the solution is subjected to a reaction with anti-digoxin on the side wall, then voltage is applied to the platinum wire leads on the two sides of the measurement device by an external voltage device, the sample solution in the open groove is operated by a magnetic tweezers device, the measurement for the residual capacity after lambda-DNA neutralization is realized, the measurement method is simple and easy, and precision of a measurement result of the residual capacity is high.

The invention provides a method for measuring and calculating the height conversion coefficient of magnetic tweezers under small force, which comprises the step of measuring and calculating the heightconversion coefficient of the magnetic tweezers by using a nucleoprotein filament formed by combining homologous recombinant protein and DNA as an absolute scale and according to the height difference before and after the nucleoprotein filament is formed and the base number of the DNA. The invention further provides application of the method. Calculation of the conversion coefficient is an important link of instrument correction and has a very important position, and recalibration needs to be carried out after instrument accessories, an objective lens and an objective lens are replaced, oil immersion is performed and a light path is adjusted. According to the invention, the cost and environmental noise required by instrument construction can be reduced, and the purpose of quickly and simply measuring and calculating the coefficient is achieved.

The invention provides a magnetic tweezer fluorescence device. The device comprises a light source, wherein visible light emitted by the light source is used for entering a sample to obtain diffractedlight of the sample; a magnet located between the light source and the sample; a laser assembly; an electric platform which is used for adjusting the direction of the laser emitted by the laser assembly and enabling the laser to enter the sample in the reverse direction opposite to the visible light; a bright field imaging device which is used for imaging the diffracted light of the sample; a fluorescence imaging device; and a fluorescence receiving device which is positioned between the sample and the light path of the electric platform, and is used for reflecting the diffracted light of thesample into the bright field imaging device, enabling the laser emitted by the electric platform to enter the sample, receiving the fluorescence emitted by the sample and reflecting the fluorescenceinto the fluorescence imaging device. According to the magnetic tweezer fluorescence device, the dynamic assembly process of biomacromolecules and the dynamic process of interaction between the biomacromolecules can be obtained at the same time.

The invention provides a sound tweezers and magnetic tweezers combined control device and control method, and the device comprises a sound tweezers assembly which is used for forming a control sound field in a control region, and controlling a control object to move in the control region; the magnetic tweezers assembly is located on one side of the sound tweezers assembly and used for forming a control magnetic field in the control area and controlling the control object to rotate at the current position; wherein the control object has magnetism. According to the control device and the control method provided by the technical scheme of the invention, non-contact accurate control on the motion of the control object is realized.

The invention discloses a method for preparing and using micron-scale magnetic tweezers. It includes the following steps: 1) drop particle-filled (magnetic particles wrapped in non-magnetic substances) nanocomposite resin onto the template substrate, and the resin penetrates into the cavity of the template substrate to form product I; 2) apply a magnetic field, and the magnetic particles in the resin Redistribute to form Product II; 3) Use light to cure the resin that is not blocked by the photomask to form Product III, and obtain cured micropillars I; 4) Exchange the magnetic poles, redistribute the magnetic particles, and then solidify to form Product IV to obtain The solidified microcolumn II; 5) peeling the product IV from the template substrate to obtain the microcolumn III mixed and distributed; 6) applying a parallel magnetic field to the microcolumn III to achieve the effect of micro tweezers. The micro tweezers prepared by the invention can pick up micron-sized particles, and the micro tweezers can be reused by applying and removing the magnetic field. In addition, the resin is filled with magnetic particles, and its hardness and rigidity are also greatly improved.

The invention discloses a magnetic tweezers probe based on an optical fiber. The magnetic tweezers probe is characterized in that: a probe core is constructed by an optical fiber of which the surface is provided with a magnetic core layer; light beams transmitted by the optical fiber can be emitted from the front end face of the probe core; and the periphery of the probe core is provided with an inner heat conducting insulating layer, a micro spiral coil, an outer heat conducting insulating layer and a shield layer from inside to outside in sequence. The magnetic tweezers probe has the advantages of small size, no need of any complex radiating structure, adjustable size and direction of a generated magnetic field, flexible movement and realization of the functions of capturing, controlling and optically operating biomolecules.

The invention discloses a magnetic tweezers probe based on an optical fiber. The magnetic tweezers probe is characterized in that: a probe core is constructed by an optical fiber of which the surface is provided with a magnetic core layer; light beams transmitted by the optical fiber can be emitted from the front end face of the probe core; and the periphery of the probe core is provided with an inner heat conducting insulating layer, a micro spiral coil, an outer heat conducting insulating layer and a shield layer from inside to outside in sequence. The magnetic tweezers probe has the advantages of small size, no need of any complex radiating structure, adjustable size and direction of a generated magnetic field, flexible movement and realization of the functions of capturing, controlling and optically operating biomolecules.

The invention discloses a portable clamping device based on a nanopore detection chip in a magnetic tweezer system. The portable clamping device comprises a left upper liquid pool, a left lower liquid pool, a C-shaped loading platform, set screws, a rubber gasket and a right liquid pool, wherein the left lower liquid pool and the right liquid pool are tightly connected and fixed through glue, and the right liquid pool is used as a solution storage container communicated with the left lower liquid pool. A silicon nitride chip is placed between the left lower liquid pool and the left upper liquid pool which are provided with the rubber gasket. Meanwhile, the assembled liquid pools are placed on the C-shaped loading platform, so that the side faces of the left upper liquid pool and the left lower liquid pool are tightly attached to the side face of the C-shaped loading platform. The rubber gasket located between the chip and the upper and lower liquid pools ensures the sealed connection between the chip and the left upper liquid pool and the left lower liquid pool, and the left upper liquid pool and the left lower liquid pool are fastened by rotating the four set screws located at the upper end of the C-shaped loading platform, so that the tight attachment between the left upper liquid pool and the left lower liquid pool is ensured. According to the portable clamping device, by utilizing the design of the combined detachable liquid pools, the positioning and clamping during the connection of the liquid pools and the chip can be conveniently, quickly, accurately and efficiently realized, so that the required chip is quickly replaced without damaging the chip.

The invention discloses a single molecule control transverse magnetic tweezer device, comprising: microscopic imaging device, magnetic tweeze device, sample holder and central monitor, where the magnetic tweezer device comprises magnetic tweezer and magnetic tweezer controller, the magnetic tweezer is horizontally arranged on the side surface of the sample tank and controlled by the magnetic tweezer controller; the sample tank is a transparent closed container, the two ends of the sample tank are provided with solution inlet and outlet, respectively, one end of a biological single molecule isconnected with the side wall of the transparent container far from the magnetic tweezer and the other end is connected with a paramagnetic ball, which is controlled by the magnetic tweezer; the microscopic imaging device is arranged at the upper or lower part of the sample tank, and the microscopic imaging device and the sample tank can horizontally and relatively translate; the microscopic imaging device transmits the obtained image information to the central monitor for data processing. And its beneficial effects: it can research and analyze biological single molecule, including related enzyme and protein, etc, and it can directly give length information of the biological single molecule and it can rotate the biological single molecule.