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Virus single particle separation method based on nano-micro composite spheres

A separation method and virus particle technology, applied in the directions of microorganism-based methods, viruses/phages, biochemical equipment and methods, etc., can solve the problem of difficulty in obtaining single virus particles, and achieve the effect of low cost

Active Publication Date: 2021-06-18
XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely small size of virus individuals, most of which are in the range of 15-200nm, it is difficult to obtain single virus particles by using these biological methods

Method used

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  • Virus single particle separation method based on nano-micro composite spheres
  • Virus single particle separation method based on nano-micro composite spheres
  • Virus single particle separation method based on nano-micro composite spheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This implementation discloses a virus single particle separation method based on nano-micro composite spheres. The chip used in this embodiment contains an array of nano-micro composite spheres, wherein the chip is a commercially available 2-inch, 50 μm thick double-sided Polished silicon wafers, the nano-micro composite balls arrayed on the chip are composed of nano-spheres embedded in nano-holes on the surface of the micro-spheres, wherein the micro-spheres are polystyrene micro-spheres with a diameter of 5 μm, the surface is modified by fluorescence, and the interior of the balls is Contains nano-magnetic particles, removes the external magnetic field, has no residual magnetism, and has no virus adsorption capacity. The nanospheres are silica nanospheres with a diameter of 1300nm. The surface is modified by amination and has virus adsorption capacity.

[0032] Single viral particles were isolated using the micropipette method as follows:

[0033] Taking GVE2 phage as...

Embodiment 2

[0036] This implementation discloses a virus single-particle separation method based on nano-micro composite spheres. The chip containing the array of nano-micro composite spheres used in this embodiment, wherein, the chip is commercially available with a thickness of 3 mm and an average filter pore size of 0.45 mm. - 0.8 μm porous ceramics, the nano-micro composite spheres arrayed on the chip are composed of nanospheres embedded in the nanopores on the surface of the microspheres, wherein the microspheres are polystyrene microspheres with a diameter of 2 μm, and the surface is modified by fluorescence , The inside of the ball contains nano-magnetic particles, there is no residual magnetism after removing the external magnetic field, and it does not have the ability to adsorb viruses. The nanospheres are silica nanospheres with a diameter of 100nm. The surface is modified by amination and has virus adsorption ability.

[0037] A droplet microfluidic method is used to separate a...

Embodiment 3

[0040] This implementation discloses a virus single-particle separation method based on nano-micro composite spheres. The chip containing the array of nano-micro composite spheres used in this embodiment, wherein, the chip is commercially available with a thickness of 3 mm and an average filter pore size of 0.45 mm. - 0.8 μm porous ceramics, the nano-micro composite spheres arrayed on the chip are composed of nanospheres embedded in the nanopores on the surface of the microspheres, wherein the microspheres are polystyrene microspheres with a diameter of 2 μm, and the surface is modified by fluorescence , The inside of the ball contains nano-magnetic particles, there is no residual magnetism after removing the external magnetic field, and it does not have the ability to adsorb viruses. The nanospheres are silica nanospheres with a diameter of 100nm. The surface is modified by amination and has virus adsorption ability.

[0041] Individual composite pellets were separated using t...

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Abstract

The invention discloses a virus single particle separation method based on nano-micro composite spheres. According to the method, a chip containing an array of the nano-micro composite spheres is taken, the nano-micro composite spheres are formed by embedding nano-spheres in nano-pores in micro-spheres, the nano-spheres have virus adsorption capacity, the surfaces of the micro-spheres do not have specific adsorption capacity to viruses, the spheres contain magnetic particles, the viruses in a virus solution are captured by using the nano-spheres on the nano-micro composite spheres to obtain virus-nano-micro sphere composite particles, and the single virus-nano-micro sphere composite particles are separated out, and therefore, single virus particles are separated out. According to the method, a micropipette method, a magnetic tweezers method or a droplet microfluidic method is combined with the nano-micro composite spheres to capture, detect and separate the single virus particles, and the method has the advantages of high efficiency, rapidness and low cost.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a virus single particle separation method based on nano-micro composite spheres. Background technique [0002] A key basic work of virus research is the need to obtain various virus particles and genome information. In conventional biology, single colonies and single cells are mainly obtained through the single colony technology of petri dish and single particle technology of optical micromanipulation, and then the genome is obtained through pure culture and molecular sequencing. However, due to the extremely small size of virus individuals, most of which are in the range of 15-200nm, it is difficult to obtain single virus particles using these biological methods. Contents of the invention [0003] In order to solve the above problems, the present invention provides a virus single particle separation method based on nano-micro composite spheres. [0004] The pres...

Claims

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Application Information

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IPC IPC(8): C12N7/02C12R1/92
CPCC12N7/00C12N2795/10151
Inventor 徐方成徐玲慧蔡佳达
Owner XIAMEN UNIV
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