Imaging magnetic tweezers device, and system and method for integrating imaging magnetic tweezers device with single-molecule fluorescence technology

Abstract

The invention discloses an imaging magnetic tweezers device, and a system and a method for integrating imaging magnetic tweezers device with a single-molecule fluorescence technology. The imaging magnetic tweezers device comprises a magnetic object lens with adjustable magnetic field gradient and magnetic field intensity, an illuminating light source and an electric coupling device. The magnetic object lens is combined with the illuminating light source and the electric coupling device to realize superparamagnetic microsphere imaging and conduct single-molecule mechanical control at the same time. On one hand, a magnetic tweezers experiment can be conduct, the magnetic tweezers device can be integrated with various single-molecule fluorescence technologies for application, not only can fluorescence signal detection be conducted to a sample, but also mechanical control can be conducted to the sample, and the fluorescence signal detection and the mechanical control are independent to each other and do not interfere with each other; and on the other hand, the imaging magnetic tweezers device can apply constraints to superparamagnetic microspheres in the sample, the Brownian fluctuation of a single-molecule connecting system can be effectively reduced, an integrated single-molecule fluorescence device can obtain staler fluorescence signals, and the experimental accuracy during single-molecule control measurement is improved.

Description

technical field [0001] The invention relates to the field of single-molecule manipulation and detection of biological macromolecules, in particular to an imaging magnetic tweezers device and a system and method integrated with single-molecule fluorescence technology. Background technique [0002] Ordinary single-molecule magnetic tweezers manipulation technology adopts the method of installing magnets and corresponding electric coupling devices in the optical microscope device to realize single-molecule manipulation. Although this method is simple and easy, the measurement accuracy is not high. Single-molecule fluorescence technology can realize single-molecule detection at the nanometer or even sub-nanometer level, but it cannot perform controllable single-molecule mechanical measurements. For biological macromolecules, we need to measure the consequences of intermolecular interactions and detect the causes of these consequences, which guides us to introduce mechanical mani...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the prior art cannot realize the organic combination of single-molecule mechanical manipulation and single-molecule fluorescence detection, the present invention proposes an imaging magnetic tweezers device and a system and method integrated with single-molecule fluorescence technology, which can realize magnetic tweezers and single-molecule fluorescence detection. Integration of fluorescence techniques and single-molecule mechanical manipulation and measurement with subnanometer precision

Images