DNA sequencing device and method

A DNA sequencing and DNA chain technology, applied in the field of DNA sequencing devices, can solve problems such as the inability to capture a single DNA, and achieve the effects of low noise level, high signal-to-noise ratio of sequencing signals, and reduced via speed

Pending Publication Date: 2019-11-15
GUANGDONG UNIV OF TECH
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the existing DNA sequencing devices, the existing probes bind a large number of DNA single strands and cannot capture a single DNA, and during the sequencing process, the existing sequencing devices can only pull the DNA single strand upwards at one end Sequencing results in severe thermal movement of DNA in solution at room temperature. The present invention provides a DNA sequencing device and sequencing method to avoid binding multiple DNA single strands on a single magnetic bead.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA sequencing device and method
  • DNA sequencing device and method
  • DNA sequencing device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Such as figure 1 As shown, a DNA sequencing device includes a three-dimensional nano-motion platform 1, a probe 3, a positioning fixture 2 for clamping and fixing the probe 3, and a comprehensive sequencing layer. The positioning fixture 2 is fixed on the three-dimensional nano-motion platform 1. The sequencing layer includes a solid nanopore chip 4, a liquid pool 5, a magnetic tweezers system 6, a power supply 10, a current measuring device 9 and electrodes 11; The center of the tower top is provided with a solid nanopore 12; the liquid pool 5 is arranged on the lower part of the solid nanopore chip 4, and the inside of the liquid pool 5 is provided with a number of magnetic beads 8, and the electrode 11 arranged on the top of the solid nanopore chip 4 passes the current measurement The device 9 is connected to the negative pole of the power supply 10, the electrode 11 arranged under the solid nanopore chip 4 is connected to the positive pole of the power supply 10, an...

Embodiment 2

[0035] Based on the DNA sequencing device, the present invention also provides a DNA sequencing method, such as image 3 As shown, the specific implementation includes the following steps:

[0036] 1) Place the solid nanopore chip 4 above the liquid pool 5, and transplant test droplets above and below the solid nanopore chip 4, the test droplets can only pass through the solid nanopore 12 of the solid nanopore chip 4 connected;

[0037] 2) binding the DNA strand to be sequenced to the tip surface of the probe 3 through the DNA binding body 32 on the tip surface of the lower part of the substrate 30 of the probe, and fixing the probe 3 in the positioning fixture 2 of the three-dimensional nano-motion platform 1;

[0038] 3) below the liquid pool 5, utilize the gradient magnetic field of the magnetic tweezers system 6 to arrange the array of magnetic beads 8, so as to receive the DNA strand from above the solid-state nanopore chip 4;

[0039] 4) Apply a voltage between the two...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a DNA sequencing device which comprises a three-dimensional nanometer movement platform, a probe, a positioning clamp for clamping and fixing the probe, and a comprehensive sequencing layer. The positioning clamp is fixed to the three-dimensional nanometer movement platform. The comprehensive sequencing layer comprises a solid nanopore chip, a liquid pond, a magnetic tweezers system, a power supply, a current measuring device and an electrode. The invention further discloses a DNA sequencing method based on the DNA sequencing device. The defects are overcome that when sequencing is conducted through an existing DNA sequencing device, a large number of DNA single chains are bound, but no single DNA can be captured, and only one end of the DNA single chain can be pulled to move up for sequencing, so DNA in a solution is prone to Brownian movement in the sequencing process; the high-precision DNA single-basic-group sequencing is realized.

Description

technical field [0001] The invention relates to the technical field of biomolecular detection, and more specifically, to a DNA sequencing device and a sequencing method. Background technique [0002] Solid-state nanopore sequencing is a very promising research direction to achieve high-sensitivity single-molecule detection of DNA or RNA molecules. It is simple, easy to integrate, and miniaturized, and has attracted extensive attention from domestic and foreign researchers. However, solid-state nanopore sequencing still faces many challenges before realizing commercial sequencing applications: 1. The speed of passing DNA molecules. The speed of DNA molecules passing through the hole driven by the current is too fast, so that the effective data points obtained by the existing patch clamp device or other signal acquisition systems are too few, and it is impossible to distinguish a single base according to the signal of blocking the current; 2. DNA in the nanopore Suppression o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/34C12M1/00C12Q1/6869
CPCC12Q1/6869C12Q2563/143C12Q2563/149C12Q2565/519
Inventor 袁志山王成勇
Owner GUANGDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap