DNA sequencing device and method

Abstract

The invention discloses a DNA sequencing device which comprises a three-dimensional nanometer movement platform, a probe, a positioning clamp for clamping and fixing the probe, and a comprehensive sequencing layer. The positioning clamp is fixed to the three-dimensional nanometer movement platform. The comprehensive sequencing layer comprises a solid nanopore chip, a liquid pond, a magnetic tweezers system, a power supply, a current measuring device and an electrode. The invention further discloses a DNA sequencing method based on the DNA sequencing device. The defects are overcome that when sequencing is conducted through an existing DNA sequencing device, a large number of DNA single chains are bound, but no single DNA can be captured, and only one end of the DNA single chain can be pulled to move up for sequencing, so DNA in a solution is prone to Brownian movement in the sequencing process; the high-precision DNA single-basic-group sequencing is realized.

Description

technical field [0001] The invention relates to the technical field of biomolecular detection, and more specifically, to a DNA sequencing device and a sequencing method. Background technique [0002] Solid-state nanopore sequencing is a very promising research direction to achieve high-sensitivity single-molecule detection of DNA or RNA molecules. It is simple, easy to integrate, and miniaturized, and has attracted extensive attention from domestic and foreign researchers. However, solid-state nanopore sequencing still faces many challenges before realizing commercial sequencing applications: 1. The speed of passing DNA molecules. The speed of DNA molecules passing through the hole driven by the current is too fast, so that the effective data points obtained by the existing patch clamp device or other signal acquisition systems are too few, and it is impossible to distinguish a single base according to the signal of blocking the current; 2. DNA in the nanopore Suppression o...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the existing DNA sequencing devices, the existing probes bind a large number of DNA single strands and cannot capture a single DNA, and during the sequencing process, the existing sequencing devices can only pull the DNA single strand upwards at one end Sequencing results in severe thermal movement of DNA in solution at room temperature. The present invention provides a DNA sequencing device and sequencing method to avoid binding multiple DNA single strands on a single magnetic bead.

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