Biological single molecule manipulation detection system and corresponding capillary based on transverse magnetic tweezers

Abstract

The invention provides a biological single molecule manipulation detection system based on a lateral magnetic tweezer. The biological single molecule manipulation detection system comprises a transparent sample groove, a magnet device, a light source and an objective lens, wherein the inner side surface of the side wall of the sample groove is used as a single molecule connection surface, the single molecule connection surface is not parallel or perpendicular to the parallel light beam emitted by the light source and used for illumination, and the axis of the objective lens is perpendicular to the direction of the force applied by the magnet device on a single molecule sample. The present invention further provides a capillary tube for the biological single molecule manipulation detection system. With the biological single molecule manipulation detection system and the capillary tube of the present invention, the short biological single molecule can be subjected to manipulation detection, the time and space resolution of the existing lateral magnetic tweezer manipulation detection can be improved, and the adverse effect of the mechanical thermal drift of the instrument on the detection results can be simply eliminated.

Description

technical field [0001] The invention relates to the technical field of biological single-molecule manipulation detection, in particular, the invention relates to a biological single-molecule manipulation detection system based on transverse magnetic tweezers and a corresponding capillary. Background technique [0002] Biological single-molecule manipulation detection technology is a technology at the level of a single molecule. This technology can realize the manipulation of biological macromolecules in cells by force or torque outside the cell, so it is being used more and more. At present, biological single-molecule manipulation detection technology is mainly used to study the physical or biochemical properties of proteins that bind to DNA or RNA and some proteins themselves. Existing single-molecule manipulation detection methods include optical tweezers, biomembrane force probes, atomic force microscopy, and magnetic tweezers, each of which has its own characteristics an...

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Problems solved by technology

Therefore, the image of shorter DNA will be covered by the above shadow, resulting in the inability to detect shorter DNA, thus making the temporal and spatial resolution of the transverse magnetic tweezers lower

[0005] To sum up, the accuracy of the existing transverse magnetic tweezers can no longer meet the accuracy requirements of some experiments. Therefore, there is an urgent need for a solution that can improve the time and space resolution and have a wider range of applications.

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