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The method and application of calculating the conversion coefficient of magnetic tweezers height under small force

A technology of conversion coefficient and measurement method, which is applied in the direction of measuring devices, optical instrument testing, and testing optical properties, etc., can solve the problems of reduced light flux, high magnetic force, and the influence of heat emission reaction system, etc., and achieves simple operation, easy acquisition, Easy-to-use effects

Active Publication Date: 2022-02-18
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

However, the limitation of this method is that it needs to use a higher magnetic force, and the data in a strong environment is required to make the fitting result the most accurate, and the strong condition needs to reduce the slit spacing of the magnet, resulting in a decrease in the amount of light passing through, and a more expensive one has to be replaced. High power LED light source
A high-power light source will increase the cost of many instruments. In addition, its heat dissipation will affect the reaction system. A fluctuation of 0.1°C will cause 5nm environmental noise to the instrument.

Method used

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  • The method and application of calculating the conversion coefficient of magnetic tweezers height under small force
  • The method and application of calculating the conversion coefficient of magnetic tweezers height under small force
  • The method and application of calculating the conversion coefficient of magnetic tweezers height under small force

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Embodiment 1

[0054] This example is used to illustrate the method of the present invention.

[0055] RecA protein is a homologous recombination protein in prokaryotes, and its first binding site can bind to single-stranded DNA. When double-stranded DNA has a single-stranded RecA protein that first binds to the single-stranded DNA, then the subsequent RecA protein can gradually extend along the single strand to the double-stranded region, and finally wraps the entire DNA. In addition, the RecA protein in the concentration When it is higher, it can also directly form nucleoprotein filaments with double strands. Nucleoprotein filaments have a periodic helical structure, one RecA monomer is combined with a length of 3 bases, and 6.2 RecA monomers are a cycle. The nucleoprotein filaments to be used in the present invention are characterized by stretching the nucleoprotein filaments below 70pN, The average distance between the bases is 0.51nm, and will not change below 70pN, such as image 3 s...

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Abstract

The invention provides a method for measuring and calculating the conversion coefficient of the height of magnetic tweezers under small force. The method includes: using the nucleoprotein filament formed by the combination of homologous recombination protein and DNA as an absolute scale, and passing the nucleoprotein filament before and after the formation of the nucleoprotein filament The height difference and the base number of DNA, the calculation of the conversion coefficient of the height of the magnetic tweezers, and its application are also provided. Calculating the conversion coefficient is an important part of instrument calibration and plays a very important role. Recalibration is required after replacing instrument accessories, objective lens, objective lens immersed in oil and adjusting the optical path. The invention can reduce the cost and environmental noise required for instrument construction, and achieve the purpose of quickly and simply measuring coefficients.

Description

technical field [0001] The invention relates to the technical field of single-molecule biophysics, in particular to a method and application for measuring and calculating the conversion coefficient of the height of magnetic tweezers under small force. Background technique [0002] Magnetic tweezers are a common method for studying single-molecule biophysics, and the research objects are usually biological macromolecules such as DNA, RNA, and proteins. In the study, one end of the biomacromolecule was attached to a paramagnetic bead and the other end was attached to a coverslip. The shape of the diffraction ring of the magnetic ball is different with the change of the height in the solution. Through the shape of the diffraction ring of the magnetic ball, we can get its position in space. At the same time, through the control of the magnetic field gradient, the force exerted by the magnet on the paramagnetic sphere can be changed, and then the force acting on the biomacromole...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01M11/02
CPCG01M11/0264
Inventor 黄星榞徐春华李明
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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