Monomolecular control transverse magnetic forceps device

Abstract

The invention discloses a single molecule control transverse magnetic tweezer device, comprising: microscopic imaging device, magnetic tweeze device, sample holder and central monitor, where the magnetic tweezer device comprises magnetic tweezer and magnetic tweezer controller, the magnetic tweezer is horizontally arranged on the side surface of the sample tank and controlled by the magnetic tweezer controller; the sample tank is a transparent closed container, the two ends of the sample tank are provided with solution inlet and outlet, respectively, one end of a biological single molecule isconnected with the side wall of the transparent container far from the magnetic tweezer and the other end is connected with a paramagnetic ball, which is controlled by the magnetic tweezer; the microscopic imaging device is arranged at the upper or lower part of the sample tank, and the microscopic imaging device and the sample tank can horizontally and relatively translate; the microscopic imaging device transmits the obtained image information to the central monitor for data processing. And its beneficial effects: it can research and analyze biological single molecule, including related enzyme and protein, etc, and it can directly give length information of the biological single molecule and it can rotate the biological single molecule.

Description

technical field [0001] The invention relates to a single-molecule manipulation magnetic tweezers device, which belongs to the field of single-molecule manipulation and detection. Background technique [0002] Many tools and techniques for studying single molecules have emerged in the past decade, for example, optical tweezers, magnetic tweezers, atomic force microscopy, glass microtubes, and more. The development of micro-force measurement technology has made it possible to study the elastic properties of nucleic acid molecules and the structural changes caused by external forces at the single-molecule level. At present, these techniques are increasingly used to study enzymes and proteins that interact with DNA, such as DNA polymerase, RNA polymerase, topoisomerase, etc. At the same time, the technique can also be used to study a series of molecular motors acting on DNA. [0003] In all of these devices, a DNA molecule or other macromolecule is attached to a surface at one...

AI Technical Summary

Problems solved by technology

Although this method can observe the fluorescence of a single molecule, the measurement of the length of a single molecule is extremely complicated and has a large error

In 1996, Strick et al. invented the vertical magnetic tweezers manipulator to measure the length of a single molecule through an optical sensor. However, since the elongation direction of the single molecule is parallel to the optical axis of the microscope objective lens, it is impossible to observe the single molecule with fluorescence.

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