Measurement device and method for residual capacity after lambda-DNA neutralization

A technology for measuring device and remaining power, applied in the direction of measuring device, measuring electrical variables, instruments, etc., can solve the problems of low accuracy of measurement results and inconvenient measurement

Pending Publication Date: 2019-06-28
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, the charged amount of λ-DNA is measured by electrophoresis method, put λ-DNA into the solution, apply voltage at both ends of the solution, let λ-DNA move in the solution, ob...

Method used

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  • Measurement device and method for residual capacity after lambda-DNA neutralization
  • Measurement device and method for residual capacity after lambda-DNA neutralization
  • Measurement device and method for residual capacity after lambda-DNA neutralization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] When λ-DNA is condensed in the spermine (spermine) solution with a concentration of 0.5mM,

[0067] Known electric current I=488 μ A, spermine (spermine) solution resistivity σ=647 μ S / cm, the cross-section D=8×10 of groove -6 m 2 ,

[0068] The stretching length of λ-DNA L=11.44μm and the deflection distance δ can be measured by real-time analysis software x =0.42μm, the corresponding F 磁 =0.95PN, get:

[0069]

[0070] ∴tanθ=0.036

[0071]

[0072] ∴F 电 =F 磁 tanθ=0.95×0.036=0.034PN

[0073]

[0074]

[0075] It is known that the charge amount of the elementary charge is 1.6×10 -19 c,

[0076] ∴ charge (indivual)

Embodiment 2

[0078] When λ-DNA is condensed in NaCl solution with a concentration of 10mM,

[0079] Known current I=980μA, NaCl solution resistivity σ=1665μS / cm, slotted cross-section D=8×10 -6 m 2 ,

[0080] The stretching length of λ-DNA L=14.85μm and the deflection distance δ can be measured by real-time analysis software x =0.3 μm, the corresponding F 磁 =1.34PN, get:

[0081]

[0082] ∴tanθ=0.02

[0083]

[0084] ∴F 电 =F 磁 tanθ=1.34×0.02=0.0268PN

[0085]

[0086]

[0087] It is known that the charge amount of the elementary charge is 1.6×10 -19 c,

[0088] ∴ charge (indivual).

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Abstract

The invention relates to a measurement device and method for residual capacity after lambda-DNA neutralization. The measurement device comprises a glass base, wherein an open groove for storing a sample solution is formed in one side of the glass base; a front block piece is arranged on one side, corresponding to the open groove, of the glass base; glass microtubes in through connection with the open groove are formed in the position, corresponding to the open groove, of the glass base; platinum wire leads extending outwards from the inside of the open groove are symmetrically arranged at thetwo ends of the open groove; anti-digoxin is attached to the side wall of one side of the open groove. The solution containing lambda-DNA is injected into the open groove of the measurement device through the glass microtubes of the measurement device, so that the solution is subjected to a reaction with anti-digoxin on the side wall, then voltage is applied to the platinum wire leads on the two sides of the measurement device by an external voltage device, the sample solution in the open groove is operated by a magnetic tweezers device, the measurement for the residual capacity after lambda-DNA neutralization is realized, the measurement method is simple and easy, and precision of a measurement result of the residual capacity is high.

Description

technical field [0001] The invention relates to a measuring device and a measuring method for remaining electricity after λ-DNA neutralization. Background technique [0002] At present, various technical methods have been applied to the study of the interaction between DNA and nucleic acid binding molecules. Traditional methods include spectroscopy, X-ray crystallography, gel electrophoresis, DNA footprint analysis, hydrodynamic techniques, and electrochemical methods. These cluster measurement methods have certain limitations in terms of sensitivity and quantitative detection. Using single-molecule technology to study the interaction between multivalent ions and DNA can measure the individual behaviors of biomolecules that cannot be achieved by traditional cluster measurements, and perform real-time and dynamic monitoring of biomolecular behaviors (including conformational changes, interactions, mutual recognition, etc.) Manipulation, regulation, etc. based on this. [0...

Claims

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Application Information

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IPC IPC(8): G01R29/24
Inventor 王艳伟祝泽栋徐紫颜席梁燕许诗雨吕方怡杨光参
Owner WENZHOU UNIVERSITY
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