Portable clamping device based on nanopore detection chip in magnetic tweezer system

Abstract

The invention discloses a portable clamping device based on a nanopore detection chip in a magnetic tweezer system. The portable clamping device comprises a left upper liquid pool, a left lower liquid pool, a C-shaped loading platform, set screws, a rubber gasket and a right liquid pool, wherein the left lower liquid pool and the right liquid pool are tightly connected and fixed through glue, and the right liquid pool is used as a solution storage container communicated with the left lower liquid pool. A silicon nitride chip is placed between the left lower liquid pool and the left upper liquid pool which are provided with the rubber gasket. Meanwhile, the assembled liquid pools are placed on the C-shaped loading platform, so that the side faces of the left upper liquid pool and the left lower liquid pool are tightly attached to the side face of the C-shaped loading platform. The rubber gasket located between the chip and the upper and lower liquid pools ensures the sealed connection between the chip and the left upper liquid pool and the left lower liquid pool, and the left upper liquid pool and the left lower liquid pool are fastened by rotating the four set screws located at the upper end of the C-shaped loading platform, so that the tight attachment between the left upper liquid pool and the left lower liquid pool is ensured. According to the portable clamping device, by utilizing the design of the combined detachable liquid pools, the positioning and clamping during the connection of the liquid pools and the chip can be conveniently, quickly, accurately and efficiently realized, so that the required chip is quickly replaced without damaging the chip.

Description

technical field [0001] The invention relates to a chip clamping device, in particular to a portable clamping device based on a nanopore detection chip in a magnetic tweezers system. Background technique [0002] With the rise of fourth-generation sequencing technology, nanopores have shown high sensitivity in the field of rapid single-molecule detection, but they still face many challenges in distinguishing DNA internal bases. Since DNA passes through the nanopore very fast, at such a speed, the current change caused by a single base is difficult to be captured by existing instruments, and sequencing of a single DNA through the nanopore in the free state cannot be achieved. A major difficulty with pore sequencing is effectively slowing down the rate at which DNA passes through the nanopore. Through the continuous exploration of many research groups, various ways have been found to reduce the speed of DNA molecules through the nanopore. , once the speed of DNA molecules pas...

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned device for clamping nanopores generally has several problems in terms of operation convenience, positioning accuracy, and reliability: 1. In terms of convenience, the old chip must be removed every time the chip is replaced, and the back of the old chip must be glued Pasted, the old chip can no longer be used

2. In terms of positioning accuracy, both the chip and the liquid pool need to be positioned visually. Although the liquid pool has concave and convex grooves for positioning, there is still a dimension that needs to be positioned visually, which may cause inaccurate positioning and cause the experiment to be relatively difficult. fail by big error

3. In terms of reliability, when the chip is replaced, the chip is exposed to the external environment. During this process, the chip is easily polluted or even damaged, which has an irreparable impact on the experiment.

At the same time, in the process of applying glue, it is required to apply evenly. If the glue is applied too little or unevenly, it will easily cause poor sealing and leakage; if too much glue is applied, the chip and the small hole will not be parallel. Difficult to separate the chip, the old chip is broken and the surface of the liquid pool is damaged

[0006] The above problems make the experimental process complicated and cumbersome, the accuracy of the experimental results decreases, and the requirements for personnel operations are high, which greatly affects the experimental efficiency and slows down the research process.

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