Magnetic sphere coordinate calibration method and device for magnetic tweezers device

A calibration method and magnetic ball technology, applied in the direction of optical devices, measuring devices, instruments, etc., can solve the problems of changing and affecting signal identification and accuracy, and achieve the effect of improving accuracy

Active Publication Date: 2019-10-22
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

[0008] However, during the long-term measurement process, the thermal drift of the magnetic tweezers device will cause the Z coordinate of the sample cell 3 to change, which will seriously affect the identification and accuracy of the signal

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  • Magnetic sphere coordinate calibration method and device for magnetic tweezers device
  • Magnetic sphere coordinate calibration method and device for magnetic tweezers device
  • Magnetic sphere coordinate calibration method and device for magnetic tweezers device

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Embodiment Construction

[0055] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0056] image 3 is the diffraction ring image of the magnetic ball in the magnetic tweezers setup. From image 3 It can be seen that the diffraction ring image of the magnetic ball is a square grayscale image (the size is N*N pixels). The diffraction ring image is converted into an N*N digital matrix, where the value of each element in the matrix corresponds to the gray value (or light intensity) of a pixel in the diffraction ring image.

[0057] First, the initial position of the centroid of the diffraction ring image of the magnetic sphere (X 0 , Y 0 ). The calculation formula of the centroid method is as follows:

[0058]

[0059] Where i, j are matrix subscripts, I ij Indicates the value of the jth element in the i-th row...

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Abstract

The invention provides a magnetic sphere coordinate calibration method and device for a magnetic tweezers device. The calibration method comprises the following steps that 1) an initial positions of the center of a diffraction ring image of a magnetic sphere is obtained in a centroid method; and 2) radial light intensity distribution of the diffraction ring image in directions of first and secondcoordinate axes is obtained, the radial light intensity distribution of the diffraction ring image in the direction of the first coordinate axis is cross-related to corresponding mirroring distribution of the image to calibrate the coordinate of the magnetic sphere in the first coordinate axis, and the radial light intensity distribution of the diffraction ring image in the direction of the secondcoordinate axis is cross-related to corresponding mirroring distribution of the image to calibrate the coordinate of the magnetic sphere in the second coordinate axis. Thus, three-dimensional coordinates of the magnetic sphere can be measured accurately by the calibration device, a loop-up table of the magnetic sphere is generated, and thermal drift of the magnetic tweezers device is corrected inreal time on the basis of the loop-up table.

Description

technical field [0001] The invention relates to a magnetic tweezers device, in particular to a calibration method and a calibration device for magnetic spherical coordinates of the magnetic tweezers device. Background technique [0002] Single-molecule magnetic tweezers manipulation technology has gradually become a popular tool in the study of biological macromolecules in recent years. It can effectively avoid the averaging effect of a large number of molecules, and select fully functional molecules for nanometer-precision manipulation and measurement. The single-molecule magnetic tweezers manipulation technology controls the magnetic force on the magnetic ball through the distance between the magnet and the superparamagnetic magnetic ball, and studies the conformational changes of biomacromolecules by monitoring the height change of the magnetic ball. [0003] figure 1 It is a structural schematic diagram of a magnetic tweezers device in the prior art. Such as figure 1 ...

Claims

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Application Information

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IPC IPC(8): G01B11/00
CPCG01B11/002G01B21/045
Inventor 王一舟李伟王鹏业翟永亮
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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