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Magnetic tweezer fluorescence device

A technology of fluorescent device and magnetic tweezers, which is applied in the field of magnetic tweezers, can solve problems such as the inability to obtain dynamic structure changes and interaction sites of biomacromolecules

Pending Publication Date: 2020-12-18
INST OF PHYSICS - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the existing magnetic tweezers technology can only obtain the dynamic structural changes of biomacromolecules, but cannot obtain the specific sites where dynamic structural changes occur and the interaction sites between biomacromolecules

Method used

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  • Magnetic tweezer fluorescence device
  • Magnetic tweezer fluorescence device
  • Magnetic tweezer fluorescence device

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0030] figure 1 It is the optical path diagram of the magnetic tweezers fluorescence device according to the preferred embodiment of the present invention. Such as figure 1 As shown, the magnetic tweezers fluorescence device includes a light source 10 , a magnet 15 , a sample box 16 , a fluorescence receiving device 20 , a motorized platform 40 , a laser component 30 , a bright field imaging device 60 and a fluorescence imaging device 70 arranged in sequence. Those skilled in the art will know that in order to clearly illustrate the optical path principle and working principle of the present invention, figure 1 Various required fixing devices, adjusting devices, loading platforms, etc. are not shown.

[0031] The light source 10 inc...

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Abstract

The invention provides a magnetic tweezer fluorescence device. The device comprises a light source, wherein visible light emitted by the light source is used for entering a sample to obtain diffractedlight of the sample; a magnet located between the light source and the sample; a laser assembly; an electric platform which is used for adjusting the direction of the laser emitted by the laser assembly and enabling the laser to enter the sample in the reverse direction opposite to the visible light; a bright field imaging device which is used for imaging the diffracted light of the sample; a fluorescence imaging device; and a fluorescence receiving device which is positioned between the sample and the light path of the electric platform, and is used for reflecting the diffracted light of thesample into the bright field imaging device, enabling the laser emitted by the electric platform to enter the sample, receiving the fluorescence emitted by the sample and reflecting the fluorescenceinto the fluorescence imaging device. According to the magnetic tweezer fluorescence device, the dynamic assembly process of biomacromolecules and the dynamic process of interaction between the biomacromolecules can be obtained at the same time.

Description

technical field [0001] The invention relates to the field of magnetic tweezers, in particular to a magnetic tweezers fluorescent device. Background technique [0002] Single-molecule magnetic tweezers manipulation technology has been successfully applied to solve major biological problems, such as using magnetic tweezers to manipulate biological macromolecules (such as chromatin), observing its dynamic folding and unfolding process under the action of magnetic force, and tracking chromatin in specific The dynamic folding and unfolding process under the action of epigenetic factors. This technique of actively dismantling chromatin by applying magnetic force and tracking its deformation under magnetic force to obtain the dynamic structure of biological macromolecules is useful for studying the structural dynamics of chromatin and different epigenetic regulatory factors for chromatin. Quality regulation provides an effective research platform. [0003] However, the existing m...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/6402G01N21/6486
Inventor 孔敬伟李伟
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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