A microfluidic chip based on Wheatstone bridge for high-throughput single-cell microtube sucking

Abstract

The invention relates to a microfluidic chip realizing high-throughout single-cell micro-tube sucking based on a wheatstone bridge, belonging to the field of microfluidic chip systems, and aiming at realizing high-throughout single-cell micro-tube sucking and measuring the single-cell mechanical property. The device simulates the wheatstone bridge structure, each unit of the device comprises fiveparts including a bridge channel and parallel channels in four parts which are divided by the bridge channel, by controlling the sizes of the five parts, the flow resistance among the five parts has certain proportional relation, then the control over the flowing direction and the like in the bridge channel is realized, further, the single-cell micro-tube sucking is realized, by controlling the input flow, the differential pressure between two ends of the cell during micro-tube sucking can be controlled, and thus the research for the single-cell mechanical property is facilitated. The microfluidic chip can be used for researching the single-cell micro-tube sucking and the single-cell mechanical property.

Description

technical field [0001] The invention belongs to the field of microfluidic chip systems, and specifically relates to a microfluidic chip device that uses the principle of fluid mechanics to realize the sucking of single-cell microtubes, and proposes a device that utilizes the principles of fluid mechanics and Wheatstone bridges to realize single-cell microtubules. New way to suck and study its mechanical properties. Background technique [0002] Cell is the basic unit of life, any deviation in its structure and physical properties may gradually destroy the integrity of cell structure, and even affect the biological function of cells. Therefore, quantitative research on cell mechanical properties is very necessary. [0003] Most of the previous studies on cell mechanical properties took the cell community as the research object, and finally obtained the average value of the characteristic parameters. However, there are differences between individual cells, and even within the...

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Problems solved by technology

[0004] The commonly used parallel plate flow chamber technology is difficult to achieve precise control of single cells; while the traditional microtube sucking technology can be used to measure the deformation ability and mechanical properties of single cells under the action of sucking pressure difference, but there are complex operations and high efficiency. Low, difficult to achieve high-throughput research and other defects

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