Mechanical dynamic regulation and control chip for single cell total environment

Abstract

The invention provides a mechanical dynamic regulation and control chip for a single cell total environment. The chip provided by the invention comprises a micro-fluid control layer, a piezoelectric vibration layer and at least one single cell pool and is characterized in that the micro-fluid control layer and the piezoelectric vibration layer are integrated on the chip; the mechanical dynamic regulation and control of a fluid medium with single cells is carried out by regulating the direction and flow velocity of the micro-fluid of the chip; and simultaneously, the mechanical dynamic regulation and control of an extracellular matrix with single cells is carried out by regulating the amplitude and frequency of the piezoelectric vibration of the chip, thereby simultaneously realizing the mechanical dynamic regulation and control of all environments with single cells on the chip. The chip provided by the invention aims to solve the problem of difficulty in dynamic regulation and controlof an environment in a single cell mechanics technique, does not have mechanical dynamic regulation dead zones, has the characteristics of simplicity, convenience and flexibility in operation and high flux, can be used for discovering the biomechanics characteristics of cells in time and space and is suitable for being applied in wide fields such as biomechanics, tissue engineering, drug screening, clinical medicine and the like.

Description

technical field [0001] The invention relates to the technical field of biological analysis, and is a chip for dynamically regulating the mechanical environment of cells. Background technique [0002] As the basic unit of life, cells constantly interact with their environment (including fluid medium and extracellular matrix). It has an important impact on life activities such as death and migration. On the other hand, an important manifestation of cell activities is that cells produce mechanical changes, which further regulate cell activities. Therefore, how cells sense the mechanical stimuli of the environment and convert them into mechanical signals and regulate cell activities, that is, cell mechanics, constitutes one of the basic issues of life activities. As a supporting platform, the development of cell mechanics technology promotes the deepening of understanding of this fundamental issue and plays an increasingly important role in improving human health. [0003] Th...

AI Technical Summary

Problems solved by technology

In addition, MAT, AFM and other technologies require sophisticated and complex equipment and devices, which are difficult to achieve integration with high-throughput chips, and there are insurmountable obstacles in the realization of high-throughput cell biomechanical analysis

At present, there is no technical report on the simultaneous dynamic regulation of fluid medium and extracellular matrix, that is, the whole cell environment.

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