Photonic crystal microscope and cell mechanics measurement method

Abstract

The embodiment of the invention provides a photonic crystal microscope and a cell mechanics measurement method. The photonic crystal microscope comprises a photonic crystal substrate, an objective table, a detection light source and an imaging assembly, wherein the photonic crystal substrate is arranged above the objective table, the detection light source and the imaging assembly are sequentiallylocated on the side, deviating from the photonic crystal substrate, of the objective table, the photonic crystal substrate is used for culturing to-be-detected cells, and when the to-be-detected cells grow on the photonic crystal substrate, the photonic crystal substrate can deform; specifically, the detection light source is used for emitting detection light to the photonic crystal substrate; the photonic crystal substrate reflects the detection light to the imaging assembly; and the imaging assembly is used for receiving the reflected light from the photonic crystal substrate for imaging soas to obtain acting force information between the to-be-detected cells and the photonic crystal substrate by utilizing an imaging graph. On the premise of ensuring the subcellular measurement precision, the flux is greatly improved, the complexity of the algorithm is simplified, the time resolution is improved, and the device cost and the experiment complexity are reduced.

Description

technical field [0001] The disclosure belongs to the technical field of crystal microscopes, and in particular relates to a photonic crystal microscope and a method for measuring cell mechanics. Background technique [0002] Cell-generated forces are key regulators of cell adhesion, signaling, and function, and they are also important drivers of morphogenetic events in development. Over the past few decades, various methods have been developed to measure these forces. While there is great interest in understanding the contributions of these forces in biology, measuring them on a broad scale remains challenging. [0003] The simplest methods of characterizing the presence of cellular forces involve measuring deformations of cells, substrates, or tissues without attempting to relate these deformations to actual forces. For example, cells embedded in a collagen gel will compress the gel, mimicking the contraction that occurs during wound healing. The size of the cell force c...

AI Technical Summary

Problems solved by technology

Therefore, high-resolution imaging is required to map high-resolution traction force, which greatly limits the application of this method.

High magnification objectives required for high resolution also limit throughput

High-dose phototoxicity will also cause greater interference to the sample to be tested

[0006] Although methods based on microcolumn arrays and methods based on fluorescence resonance energy transfer have been greatly developed, there are still limitations such as preparation difficulties and high requirements for measurement equipment.

In addition, the current method is limited to the mechanical measurement of macroscopic tissue or several microscopic cells, and cannot perform mechanical measurement with subcellular resolution within the millimeter field of view.

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