Cell mechanical property tester based on STM32

Abstract

The invention discloses a cell mechanical property tester based on STM32. The cell mechanical property tester based on STM32 comprises a quartz crystal receiving device, a frequency generation part, afrequency collecting part and a microcomputer processing part, wherein the quartz crystal receiving device comprises a quartz crystal and a film electrode; the frequency generation part consists of an oscillation circuit, a filtering circuit and an AGC (automatic gain control) circuit which are sequentially connected; the quartz crystal receiving device is connected with the oscillation circuit through an electric conducting wire; the frequency collecting part comprises a frequency / voltage conversion circuit; the microcomputer processing part comprises an STM32 chip and an external interfacethereof; the frequency / voltage conversion circuit is connected with the STM32 chip through an electric conducting wire. The cell mechanical property tester based on STM32 has the advantages that by adopting the non-invasion and non-marking testing type, the real-time, continuous and long-time testing is realized; the cell mechanical property tester is suitable for the cell mass, and the study andquantitative testing on the cell function can be performed.

Description

technical field [0001] The invention relates to a tester for cell mechanical properties, in particular to a tester for cell mechanical properties based on STM32. Background technique [0002] Quartz crystal microbalance (QCM) technology is a sensor measurement technology developed in the 1960s, which is widely used in molecular biology, microchemistry, environmental science and other detection fields. It uses the piezoelectric effect of the crystal to convert the tiny mass change on the quartz crystal electrode into a change in the vibration frequency that can be measured. Relevant studies have shown that at any time during the cell adhesion process, the surface stress or cell traction ΔS (unit: dyn / cm) generated or imposed on the crystal will cause the quartz crystal to produce a frequency shift Δf. This provides a relevant theoretical basis for testing the mechanical properties of cells with quartz crystals. [0003] The mechanical properties of cells are important param...

AI Technical Summary

Problems solved by technology

There are certain limitations in traditional animal cell mechanics methods: ① most of them are non-destructive and need to exert a certain force on the cells to realize the measurement, and the external force will lead to complex cell responses; ② most of them can only measure the mechanical properties of cells, Not suitable for cell populations; ③It is difficult to distinguish the mechanical properties of different cell structures; ④It cannot realize the non-destructive and long-term monitoring of cell mechanical parameters and realize the research and quantitative testing of cell functions

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