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Spring nanowire detector for cell mechanics detection and detection method thereof

A nanowire and detector technology, which is applied in the field of cell mechanics testing, can solve the problems of high preparation cost of atomic force microscope needle tip testing, inability to realize probe shape design, large cell damage, etc., and achieve excellent controllability of diameter and shape , good biocompatibility, low price effect

Active Publication Date: 2020-09-22
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Apparently, a large number of arrayed nanowires acting on cells at the same time limits the resolution of mechanical detection, and the insertion into the cell will cause greater damage to the cell.
However, the preparation cost of the atomic force microscope tip test is high, and it is impossible to design the shape of the probe to meet different test requirements.

Method used

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  • Spring nanowire detector for cell mechanics detection and detection method thereof
  • Spring nanowire detector for cell mechanics detection and detection method thereof
  • Spring nanowire detector for cell mechanics detection and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment provides a nanowire spring detector for cell membrane penetration detection and its preparation method, such as Figure 1-2 As shown, the specific steps include:

[0033] A. The silicon oxide wafer substrate material is cleaned, and the columnar guide steps are obtained by polystyrene ball-assisted Bosh process etching, and then placed in a plasma-enhanced chemical vapor deposition system, using low-melting indium as a catalyst, through Vertical 3D nanowire springs for growing nanowires at low temperature by planar solid-liquid-solid (IPSLS) growth mechanism. The diameter of the nanowire in this embodiment is 50 nanometers, the inner diameter of the spring is 1 micron, and the number of coils is 4.

[0034] B. Cut the end of the vertical three-dimensional nanowire spring on the substrate obtained in step A by photolithography and etching technology to obtain a nanowire structure with a straight end, and transfer it to a new substrate And carry out susp...

Embodiment 2

[0041] This embodiment provides a method for detecting the force required to penetrate the cell membrane with a calcium ion fluorescently labeled nanowire spring, such as figure 1 ,3 As shown, the specific steps include:

[0042] A. Clean the silicon oxide wafer substrate material, etch the wavy guide steps on the surface of the planar substrate, and then place it in a plasma-enhanced chemical vapor deposition system, using indium with a low melting point as a catalyst, through the planar solidification process. Growth of horizontal nanowire wavy linear springs at low temperature by liquid-solid (IPSLS) growth mechanism. The diameter of the nanowire is 80 nanometers, the inner diameter of the spring is 5 microns, and the number of coils is 5;

[0043] B. Cut off the end of the horizontal nanowire wavy linear spring on the substrate obtained in step A by photolithography and etching technology to obtain a nanowire structure with a straight end, and transfer it to a new substr...

Embodiment 3

[0047] This embodiment provides a method for hook-shaped nanowire springs to detect cell weight, such as figure 1 , 4 As shown, the specific steps include:

[0048] A. Clean the silicon oxide wafer substrate material, etch the wavy guide steps on the surface of the planar substrate, and then place it in a plasma-enhanced chemical vapor deposition system, using indium with a low melting point as a catalyst, through the planar solidification process. Growth of horizontal nanowire wavy linear springs at low temperature by liquid-solid (IPSLS) growth mechanism. The diameter of the nanowire is 60 nanometers, the inner diameter of the spring is 3 microns, and the number of coils is 5.

[0049] B. Cut off the end of the horizontal nanowire wavy linear spring on the substrate obtained in step A by photolithography and etching technology to obtain a hook-shaped nanowire structure and transfer it to a new substrate Overhanging and fixed.

[0050] C. Fix the hook-shaped nanowire spr...

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Abstract

The invention discloses a spring nanowire detector for cell mechanics detection. The spring nanowire detector comprises a vertical three-dimensional nanowire spring or a horizontal wavy nanowire typenanowire spring of which one end is fixed on a micromechanical operation platform, and the tail end of the other end of the vertical three-dimensional nanowire spring or the horizontal wavy nanowire type nanowire spring is of a linear or hook-shaped suspended structure; and when the tail end of the other end of the vertical three-dimensional nanowire spring or the horizontal wavy nanowire spring is linear, a fluorescent marker is arranged on the surface of the tail end of the linear nanowire. Related mechanical properties can be tested by controlling the real-time deformation observation of the nanowire spring, and the suspended nanoscale probe is arranged at the end part so that cell damage-free detection can be realized.

Description

technical field [0001] The invention relates to the field of cell mechanics testing, in particular to a spring nanowire detector for cell mechanics testing and a method for testing cell mechanics. Background technique [0002] Detection of cell-related mechanical properties, including weight, force required to penetrate cell membrane, deformation of cell membrane and cytoskeleton, cell adhesion, etc. Internal substances (ion channels, proteins, genetic material) are of great significance, and help to understand the mechanism of external force stimuli on changing the state of cells and manipulating cell behavior (such as drug delivery, gene regulation). Studies have shown that the mechanical interaction between cells and the outside world is mainly the structure of the cell membrane and the structure of the extracellular matrix. The cell membrane is a 6nm-thick elastic membrane composed of a layer of phospholipids, which provides a natural barrier between cells and the outsi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N21/64G01N33/487B82Y40/00
CPCG01N21/6458G01N33/4875B82Y40/00G01N2015/1006G01N15/1023
Inventor 余林蔚刘宗光严江王军转
Owner NANJING UNIV
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