Dynamic non-contact cell mechanical stimulation loading device

Abstract

The invention provides a dynamic non-contact cell mechanical stimulation loading device. A plurality of pipelines are formed in a wall of a culture cavity to be communicated with a circulation water bath; the culture cavity is fixed in a box body, and the box body is filled with heat insulation materials; the output of an air compressor and a CO2 pot is input into the culture cavity after being subjected to matching control through a gas flowmeter; a plurality of humidifying vessels are placed in the culture cavity, and a humidity measuring instrument is used for detecting the humidity inside the culture cavity; two same rotary plates rotate around a perpendicular rotating shaft under the drive of a motor, each rotary plate is provided with a permanent magnet, an object stage is installed between the two rotary plates, an experiment object is placed on the object stage, and the object stage is fixed instead rotating. Various dynamic non-contact cell mechanical stimulation loading ways can be achieved, space non-contact extrusion type, periodic gradient type and multi-dimensional uniform type mechanical stimulation effects are applied to cells, and an in-vitro cell culture environment is built for the cells.

Description

technical field [0001] The invention belongs to the field of biomechanics and relates to a cell mechanical stimulation loading device. Background technique [0002] Cells in living organisms are subject to mechanical stimuli all the time. Skeletal movement produces stretching, contraction, and twisting effects on cells when playing ball, and the peristalsis of digestive tract and shearing force caused by body fluid flow when eating. As the basic unit of life, cells only rely on a thin and soft cell membrane as a barrier to the outside world. The cytoskeleton and intracellular matrix in the cell provide certain mechanical support for it. In different mechanical environments, the growth of cells Life activities such as growth, proliferation, and differentiation are all significantly affected. Altman GH et al. found that applying tensile strain alone to cells in three-dimensional culture conditions can induce bone marrow mesenchymal stem cells to differentiate into ligament-li...

AI Technical Summary

Problems solved by technology

Cell research under magnetic field force includes static magnetic field force research under permanent magnetic field and electromagnetic field. At present, there is still a lack of cell culture research under dynamic magnetic field force. However, the environment in which organisms are complex and changeable, cell growth and differentiation under dynamic magnetic field force A series of changes will occur in physiological processes such as growth and proliferation, and static research can no longer meet the needs of scientific research.

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