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Screening method for anaerobe capable of degrading chlorophenol

A screening method, the technology of anaerobic bacteria, applied in the field of microbiology, can solve the problems such as inability to screen, and achieve the effect of easy operation and simple process

Inactive Publication Date: 2006-09-06
TONGJI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

It can be seen that other anaerobic culture methods are generally only used for the cultivation and observation of anaerobic bacteria due to their own characteristics, and cannot perform the necessary operations required for screening

Method used

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  • Screening method for anaerobe capable of degrading chlorophenol
  • Screening method for anaerobe capable of degrading chlorophenol
  • Screening method for anaerobe capable of degrading chlorophenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] see figure 1 , 2 and 3.

[0017] This implementation mode is carried out according to the following steps:

[0018] A. Screening of anaerobic granular sludge: take good-quality granular sludge from the anaerobic reactor that treats high-concentration organic wastewater such as citric acid wastewater, sieve and discard flocculent sludge, and retain particles with a particle size greater than 8mm sludge;

[0019] B. Acclimatization of anaerobic granular sludge degraded by chlorophenols: using a laboratory-scale upflow anaerobic granular sludge reactor (UASB), put the good-quality anaerobic granular sludge enriched in step A into UASB for For cultivation and acclimatization, the nutrient solution of glucose, trace elements and growth factors is pumped into the UASB from the bottom with a peristaltic pump to domesticate the anaerobic granular sludge. The temperature sensor is controlled by a temperature controller at a constant temperature of 35°C, and is pumped into th...

Embodiment 2

[0024] A, the collection of anaerobic strains: select the deep soil (anaerobic bacteria play main degradative effect) that is polluted by chlorophenols wastewater;

[0025] B. The domestication process of anaerobic granular sludge degraded by chlorophenol is the same as that of Example 1. Use pre-prepared deoxygenated sterile water to prepare sludge storage solution in a 50-500ml glass wide-mouth reagent bottle (the liquid volume is 1 / 3 of the reagent bottle), and quickly put about 100mg of chlorophenol-contaminated deep soil into the glass wide-mouth reagent bottle. Seal the mouth of the bottle; the composition of the sludge storage solution can refer to the following composition, g / L: NaHCO 3 , 0.5; Sodium acetate, 0.5; NH 4 HCO 3 , 0.01; KH 2 PO 4 , 0.005; yeast, 0.1; put the sludge sample into the anaerobic glove box, then transfer the sludge in the sampler to a 10ml sterile plastic centrifuge tube, fully stir the sludge liquid with a glass rod to make the sludge evenl...

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PUM

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Abstract

The method uses the anaerobic glove case and many modified culture medium to screen the anaerobic bacteria which possesses the highly effective character of degrading chlorophenol and acid resistance. The method comprises the following steps: A. screening the anaerobic particle sludge; B. segregation and purifying bacteria; C. primary screening the anaerobic bacteria degrading chlorophenol; D. second screening: segregation and purifying with the solid-liquid culture medium, until the cell shape being the single thallus. The operation is simple, and the theory meaning and economic value are important. The method provides the theory basis for anaerobic biological treatment process of chlorophenol waste water. The method can be used to separate, screen and culture the chlorophenol degradation bacterium, and provide the good screening method for researching chlorophenol degradation rule and interaction between function bacteria.

Description

technical field [0001] The invention relates to a method for screening anaerobic bacteria capable of degrading chlorophenols. belongs to the field of microbiology. Background technique [0002] Chlorophenols are a class of chlorinated hydrocarbons, insecticides or antimicrobial agents used for non-agricultural insecticides. The concentration threshold of its toxic effect on the human body is only 40ppm, the threshold of its toxic effect on fish is 1ppm, and 1ppb of chlorophenol in water can cause water pollution. Listed by the US EPA as Priority Pollutants and China's Blacklist of Key Pollutants. At present, the ultrafiltration method, chemical precipitation method, biological method and other treatment methods commonly used for chlorophenols have high energy consumption, high cost, and secondary pollution caused by chemical addition. Therefore, the anaerobic biological treatment technology of p-chlorophenol, a refractory organic matter, will pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/28
Inventor 陈玲张文傅以钢黄爱群夏四清
Owner TONGJI UNIV
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